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Defb29 (NM_001001444) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219922
Defb29 (Myc-DDK-tagged) - Mouse defensin beta 29 (Defb29)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Defb29 or NM_001001444"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Defb29
Synonyms 0610030I09Rik; BD-29; mBD-29
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219922 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCAGTCACAAAGTCATACTTCATGACTGTGGTGGTCGTGCTGATCCTGGTTGACGAGACCACAGGTG
GCCTGTTCGGATTCCGCAGTAGCAAGAGGCAGGAGCCATGGATTGCGTGTGAGCTTTACCAGGGGTTATG
CAGAAATGCCTGCCAGAAATATGAGATCCAGTACTTAAGTTGCCCAAAGACCAGGAAGTGCTGCCTCAAA
TATCCTAGGAAGATTACCAGCTTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219922 protein sequence
Red=Cloning site Green=Tags(s)

MPVTKSYFMTVVVVLILVDETTGGLFGFRSSKRQEPWIACELYQGLCRNACQKYEIQYLSCPKTRKCCLK
YPRKITSF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001001444
ORF Size 234 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001001444.2, NP_001001444.1
RefSeq Size 405 bp
RefSeq ORF 237 bp
Locus ID 75400
UniProt ID Q8BGW9
Cytogenetics 2 G3
MW 9.1 kDa
Summary Has antibacterial activity.UniProtKB/Swiss-Prot Function
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"NM_001001444" in other vectors (4)
SKU Description Size Price
MC211622 Defb29 (untagged) - Mouse defensin beta 29 (Defb29), (10ug) 10 ug
$150.00
MG219922 Defb29 (tGFP-tagged) - Mouse defensin beta 29 (Defb29), (10ug) 10 ug
$350.00
MR219922L3 Lenti ORF clone of Defb29 (Myc-DDK-tagged) - Mouse defensin beta 29 (Defb29) 10 ug
$450.00
MR219922L4 Lenti ORF clone of Defb29 (mGFP-tagged) - Mouse defensin beta 29 (Defb29) 10 ug
$450.00

Plasmid Map for MR219922

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file-download TXT (FASTA format)

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Cloning scheme for MR219922
Circular map for MR219922

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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