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Gpr142 (NM_181749) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219910
Gpr142 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 142 (Gpr142)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gpr142
Synonyms PGR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219910 representing NM_181749
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCACCTGAATTCAAATCCCAACAGCTACATCTGTGACGCCTACCAACATGCTGATCTTCTATGGTCCT
TGTCTCCTCATGTGTTAACAAAAGCTGTGCAGCCACAAGTGACCCTGCTGCCTACAGTCAATGGTAGTAA
CCCAAGATACGATGGTGTGGATGGCCACTGGCCAGAGAGCCCAGAGAGGTCACCCTGTGTGGCTGGTATC
ATACCTGTCATCTATTACAGCGTCCTGCTGAGCCTGGGGCTGCCTGTGGCACTGGCCCGTCTTGCTGCCA
GGACCAGGAAACCCTCCTATCACTATCTCCTGGCACTCACGGCTTCAGATATCGTCACCCAAGTGATCAT
CGTCTTCGTAGGCTTCCTCCTGCAGGGAGCTGTGCTGGCCCGCCAGGTGCCCCAGGCCGTGGTGCGCACA
GCTAACATCCTGGAGTTTGCTGCCAATCACGCCTCAGTCTGGATTGCAGTCTTGTTCACGGTGGATCGCT
ACAATGCCCTGTGCCGCCCTCTACGCCATCGGGCCACCTCGTCCCCAGGCCGGACCCACCGCGCTATTGC
TGCTGTCATTGGCGTTACCCTCCTGACCGGCATCCCATTCTACTGGTGGCTGGATGTGTGGAGGGATGCA
GACCCTCCTAGCACTATGGACAAACTCCTCAAGTGGGCTCACTGCCTCATCGTCTACTTCATTCCCTGCA
ATGTTTTCCTGGTCACCAACTCTGCCATCATCCTTCGGCTACGGAAGAGAGGCCAGAGAGGGCTGCGGCC
ATTGGTGAGCAAGAGCACAGCCATCCTCCTGGGTGTCACCTCCCTCTTCGCCCTCCTCTGGGCACCACGT
ATTATTGTCATGCTGTACCATCTGTATGTGGCCCCTGTTCACCGGGATTGGAGGGTCCACCTGGCCTTGG
ACATAGCCAACATGTTGGCCATGCTCAACACAGAGGTCAACTTTGGCCTCTATTGCTTCATCAGCAAGAC
TTTCAGAGCCACTGTCCGACAGGTCATCTGTGATGTCCATATGGCTTGCGCCTTAAAGTCACAGCCAAAG
CAAACGGTGGTGGAACTCATGCTGAAGTCCGTAGGGACAGAGCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219910 representing NM_181749
Red=Cloning site Green=Tags(s)

MHLNSNPNSYICDAYQHADLLWSLSPHVLTKAVQPQVTLLPTVNGSNPRYDGVDGHWPESPERSPCVAGI
IPVIYYSVLLSLGLPVALARLAARTRKPSYHYLLALTASDIVTQVIIVFVGFLLQGAVLARQVPQAVVRT
ANILEFAANHASVWIAVLFTVDRYNALCRPLRHRATSSPGRTHRAIAAVIGVTLLTGIPFYWWLDVWRDA
DPPSTMDKLLKWAHCLIVYFIPCNVFLVTNSAIILRLRKRGQRGLRPLVSKSTAILLGVTSLFALLWAPR
IIVMLYHLYVAPVHRDWRVHLALDIANMLAMLNTEVNFGLYCFISKTFRATVRQVICDVHMACALKSQPK
QTVVELMLKSVGTEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_181749
ORF Size 1095 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_181749.1, NP_861414.1
RefSeq Size 1098 bp
RefSeq ORF 1098 bp
Locus ID 217302
UniProt ID Q7TQN9
Cytogenetics 11 E2
MW 41.2 kDa
Summary Orphan receptor.UniProtKB/Swiss-Prot Function
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"NM_181749" in other vectors (4)
SKU Description Size Price
MC212665 Gpr142 (untagged) - Mouse G protein-coupled receptor 142 (Gpr142), (10ug) 10 ug
$457.00
MG219910 Gpr142 (tGFP-tagged) - Mouse G protein-coupled receptor 142 (Gpr142), (10ug) 10 ug
$489.00 $657.00
MR219910L3 Lenti ORF clone of Gpr142 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 142 (Gpr142) 10 ug
$757.00
MR219910L4 Lenti ORF clone of Gpr142 (mGFP-tagged) - Mouse G protein-coupled receptor 142 (Gpr142) 10 ug
$757.00

Plasmid Map for MR219910

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219910
Circular map for MR219910

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