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Cep68 (NM_172260) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219870
Cep68 (Myc-DDK-tagged) - Mouse centrosomal protein 68 (Cep68)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $671.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cep68
Synonyms 6030463E10Rik; AI481761; BC027174; Kiaa0582
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219870 representing NM_172260
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCCTGAGTGAAGATGAGGCTGAGGCAGAAGTGTCTGTCAACACAAAGGTCCCATCCTGTGGCAGGT
GGAACTCTGGGAAGCTGCTGCCCTCAGGGCTTGAACCAGATCAGCCCCTACATCTGGGTGTTGAGGGGGG
GCCACTGTGGAGGGCTGAAGCTGACCCTGGCTGCATCTCGGGAGTTTTCTTGTCACGGGTCCATACTGCC
AGCAAAGAACCAGTAGCAGATAGGTCTAAGCCTCCCCTCAGAGGCCCGCTTCCTTCTGCCAGTGTGGGCA
CTGGAGAGGTTTTACACTCTATGGGCAGCCAAATGGAGGAAGACAGGCTTCCTGCCTCCCAGGACCTACT
TCCAGCTCTTCAAGTCTTTGGAACTATAACTGTTTGCTCAGGACAGGAGGCTGACAGTGAAGACTTCCAG
GCTACGCTTGATCCATCTCAGGTGTTGGGCCTCAGCCAGCAACCACACACCTCAGGTCTCCCTCTCCCGC
CACAGTGGAAGTCCACGGTGAGCCCAGGTGCTCCTCAGCTTTCTAGCAGAAGCATCTCTGCCTCCTCGGT
GGGCAGCAGTCTTCAGGATCACCAAGAGAAGGCAGGACCTCAGAGGGCCTCTTTTGCCAATGTCTCCTCT
CCCGAGTTGACTGTACCCCAGGCAGCCCACTCTGTGGTGGGGGCAGGGCCTCCGCTCCAGGGGTCAGCAC
AGCCTCTGACCTCTGGCAGTGATGCTACAGGCCTGGGTAAGAGACACCTCTCCTTTCAGGCAGAATACTG
GGCCTGTGCGCTGCCAAATTCTCTGCCTCCTTCCCCTAACCGCCACTCCGCACTCTGGGACCCAAATAAA
GAGTATGAAGATCTGCTTGACTACACTTACCCACTGAGGCCTGGGCCTCAGCTCCCAAAGCAACCTGAAA
GTCATGTCCTGACTGAGCCTGTTCTGCAGGACTCAGGTGTAGATCTGGACAGTTTGTCTGTCTCCCCAGC
AAGTACTCTAAAGTCACCCACTAATGTCTCCCACAATTGCTCATCAGCAGAAGTGCCTACTCTGCCATTT
TCTGGAGCCAGAGAGTCATGTCTTAAGCGCTGGCCCTTGGGAATATTCCAGAAACAGGGTGGCACAAGCT
TGTCATCCTGGAACCAGCTTGCATCAACCCCTAGAGCCCCAGGCACTGAAGATGCTTCTTGGGAGAACAG
AGAGGCAGCCCTGAGGGGCACAGCGGAGGACTGCCTCCCTATAGGTGAGGACCTCCGAATGGGCTCTCCC
CAGCTGAAGACGAAGGAGAAAGAGCCACCCTTTCCCAGACAGAAGAGAGGCAGGCAGCATGTTAGCTGCC
CGGCCTGTGTGACGCCTGGATGGCCATCAGAAGAGGAGGTGGGAAGTGATGAGGAGTACCTTGCTCTGCC
CACCAGGCTGACTCAGGTCTCTAGTTTGGTGTCCTACTCAGGTGCCAGGCCCTCCTTTGTGAACCTACAC
ACTGGGGCTGCTGAGGAACACAGTTCACTACAAGTCTCAGACAGTGACAAGCCAGCCTCCCCCACATTGG
ACTCCAGCCACAGGAAGCATCCTTCTGGAACTTCCTTTCAAGGGCCTGTGGGCCAGAACCCCTGCTTCAG
GCATTCTATCCAGCCCCAGGACTCTAGAGGCAAAAGTAGTCTGATGAGTAACCAGACCCTCGGGGTCTCT
TCAAAACCACTGAAAACTCAGCCCGCCTCAAAAGCCATGACGGACAGGCGGCTGTTCTCCGAGCTAGTAG
CTGGAGAGACACTTCCCAGGACGACAGATGAGCAGGAGAAAGCTTCTCTAGTGCAGTGTGTGCAGACATT
TTGCTGTCGGCTGGAAGAGCTGATCTGCTGGTTGTATAATGTCACAGATGTTGCTGACCTCAGTGCTCCA
CCCAGGACCAGCCTTACAGGTCTCAAGTCTTCTCTGCAGCTTTACAGGCAATTTAAGAAAGATGTAGACG
AACATCAGTCCCTGACAGAGAGCGTCTTGGAGAAGGGAGAGATTCTTCTTCAGTGCCTGTTGGATAACAC
CCCAGTTTTAAAGGATGTCCTTGAGAGGATTGCAAAGCAGTCTGGTGAGCTGGAGAGCCGTGCAGATCAT
CTTTATGACTCTATCTTAGCCTCTTTGGACATGTTGGCTGGCTGCACCCTCATCCCTGACAACAGGCCAA
CAGCAGCAGAACACCCACATGAAGGGCTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219870 representing NM_172260
Red=Cloning site Green=Tags(s)

MALSEDEAEAEVSVNTKVPSCGRWNSGKLLPSGLEPDQPLHLGVEGGPLWRAEADPGCISGVFLSRVHTA
SKEPVADRSKPPLRGPLPSASVGTGEVLHSMGSQMEEDRLPASQDLLPALQVFGTITVCSGQEADSEDFQ
ATLDPSQVLGLSQQPHTSGLPLPPQWKSTVSPGAPQLSSRSISASSVGSSLQDHQEKAGPQRASFANVSS
PELTVPQAAHSVVGAGPPLQGSAQPLTSGSDATGLGKRHLSFQAEYWACALPNSLPPSPNRHSALWDPNK
EYEDLLDYTYPLRPGPQLPKQPESHVLTEPVLQDSGVDLDSLSVSPASTLKSPTNVSHNCSSAEVPTLPF
SGARESCLKRWPLGIFQKQGGTSLSSWNQLASTPRAPGTEDASWENREAALRGTAEDCLPIGEDLRMGSP
QLKTKEKEPPFPRQKRGRQHVSCPACVTPGWPSEEEVGSDEEYLALPTRLTQVSSLVSYSGARPSFVNLH
TGAAEEHSSLQVSDSDKPASPTLDSSHRKHPSGTSFQGPVGQNPCFRHSIQPQDSRGKSSLMSNQTLGVS
SKPLKTQPASKAMTDRRLFSELVAGETLPRTTDEQEKASLVQCVQTFCCRLEELICWLYNVTDVADLSAP
PRTSLTGLKSSLQLYRQFKKDVDEHQSLTESVLEKGEILLQCLLDNTPVLKDVLERIAKQSGELESRADH
LYDSILASLDMLAGCTLIPDNRPTAAEHPHEGL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172260
ORF Size 2199 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172260.3, NP_758464.2
RefSeq Size 4810 bp
RefSeq ORF 2202 bp
Locus ID 216543
UniProt ID Q8C0D9
Cytogenetics 11 12.92 cM
MW 78.7 kDa
Summary Involved in maintenance of centrosome cohesion, probably as part of a linker structure which prevents centrosome splitting. Required for localization of CDK5RAP2 to the centrosome during interphase.UniProtKB/Swiss-Prot Function
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"NM_172260" in other vectors (4)
SKU Description Size Price
MC221195 Cep68 (untagged) - Mouse centrosomal protein 68 (Cep68), (10ug) 10 ug
$739.00
MG219870 Cep68 (tGFP-tagged) - Mouse centrosomal protein 68 (Cep68), (10ug) 10 ug
$871.00
MR219870L3 Lenti ORF clone of Cep68 (Myc-DDK-tagged) - Mouse centrosomal protein 68 (Cep68) 10 ug
$971.00
MR219870L4 Lenti ORF clone of Cep68 (mGFP-tagged) - Mouse centrosomal protein 68 (Cep68) 10 ug
$971.00

Plasmid Map for MR219870

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219870
Circular map for MR219870

file-alt Citations

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