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Slc2a6 (NM_172659) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219710
Slc2a6 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 6 (Slc2a6), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Slc2a6
Synonyms A330096C23; F630103L12Rik; Glut6; Glut9
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219710 representing NM_172659
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGAACCCCTGCTAAGAACCGAGGGACTCGACTATGATACCTTCCCCGAGGTGCCCGCGACTCCTG
GAGAGAGAGAACGAGCCGGGGCCTTGAAAAACAGAAGGGTGTTCCTGGCTACCTTCGCCGCTGTGCTGGG
CAATTTCAGCTTTGGGTATGCCTTGGTCTACACATCTCCAGTCATCCCTGAACTGAAGCTCTCTTCTGAC
CCAGCACTACACCTGGACAAAATCCAGGCATCCTGGTTTGGGTCCGTGTTCACCTTGGGTGCCGCAGCAG
GGGGCCTCAGTGCTATGTTACTCAATGACCTCCTGGGCCGGAAGCTCAGCATCATGTTCTCAGCTGTCCC
CTCAGCCATTGGCTATGCAATCATGGCCGGTGCCCGTGGCCTCTGGATGCTTCTGTTGGGGAGGATGCTG
ACGGGCTTTGCCGGGGGACTCACTGCTGCCTGCATCCCGGTGTACGTGTCTGAGATTGCACCCCCTGATG
TTCGTGGGGCCCTGGGGGCCACACCGCAGCTCATGGCCGTGTTTGGATCCCTGTCTCTCTACGCCCTTGG
TCTGCTGCTACCTTGGCGATGGCTTGCTGTGGCTGGAGAGGGGCCGGTTCTCATCATGATCCTGCTGCTC
AGCTTCATGCCCAACTCTCCTCGCTTCTTGCTCTCAAAGAGCCGGGATGAGGAGGCACTGCAGGCGCTGA
CCTGGCTGCGAGCTGACTCCGAGGTCCATTGGGAGTTTGAGCAGATCCAGGACAACGTGCGGAGACAGAG
TAGCCGAGTGTCGTGGGCGGAAGCCCGGGAACCCCGTGTGTACCGGCCTGTTCTCATTGCAGTACTGATG
CGCTTTCTGCAGCAGCTGACAGGCATCACACCCATCCTGGTGTACCTACAGACCATCTTCGACAACACAT
CCGTGGTGCTGCCCTCCCAGCAGGATGCAGCGATAGTTGGTGCTGTGAGGCTCCTATCTGTGCTGATTGC
TGCTGTCACCATGGACCTGGCGGGCCGAAAAGTTCTGCTCTATGTGTCAGCATCCGTCATGTTTGCTGCC
AACCTGACACTGGGGCTCTACGTCCAGTTTGTGCCAAGGCCTCTGACCCCCAACAGCACTGTGGAGATCG
TGACCCTGGGGGACACAGCCTTCAACTATCTTACCCTGATACCCCTGCTGGCCACCATGCTCTTCATTAT
GGGCTATGCCATGGGCTGGGGTCCCATCACCTGGCTCCTCATGTCTGAGGTTCTGCCCTTGCGTGCCCGT
GGTGTGGCCTCAGGGCTCTGCGTGCTGGTCAGCTGGCTCACAGCCTTCGTCCTCACTAACTACTTCCTGC
TGGCAGTGAATGCCTTCGGCCTCCAGGTGCCTTTCTTCTTCTTCTCGGCCATCTGCCTGCTCAGCTTGCT
CTTCACAGGCTGCTGTGTGCCTGAGACCAGGGGCCGCTCGCTGGAGCAGATCGAGGCCTTCTTCCACACT
CGCAGGATGTCCTTCAGGCCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219710 representing NM_172659
Red=Cloning site Green=Tags(s)

MQEPLLRTEGLDYDTFPEVPATPGERERAGALKNRRVFLATFAAVLGNFSFGYALVYTSPVIPELKLSSD
PALHLDKIQASWFGSVFTLGAAAGGLSAMLLNDLLGRKLSIMFSAVPSAIGYAIMAGARGLWMLLLGRML
TGFAGGLTAACIPVYVSEIAPPDVRGALGATPQLMAVFGSLSLYALGLLLPWRWLAVAGEGPVLIMILLL
SFMPNSPRFLLSKSRDEEALQALTWLRADSEVHWEFEQIQDNVRRQSSRVSWAEAREPRVYRPVLIAVLM
RFLQQLTGITPILVYLQTIFDNTSVVLPSQQDAAIVGAVRLLSVLIAAVTMDLAGRKVLLYVSASVMFAA
NLTLGLYVQFVPRPLTPNSTVEIVTLGDTAFNYLTLIPLLATMLFIMGYAMGWGPITWLLMSEVLPLRAR
GVASGLCVLVSWLTAFVLTNYFLLAVNAFGLQVPFFFFSAICLLSLLFTGCCVPETRGRSLEQIEAFFHT
RRMSFRP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172659
ORF Size 1491 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172659.2, NP_766247.2
RefSeq Size 2136 bp
RefSeq ORF 1494 bp
Locus ID 227659
UniProt ID Q3UDF0
Cytogenetics 2 A3
MW 54.9 kDa
Summary Probable sugar transporter that acts as a regulator of glycolysis in macrophages (PubMed:30431159). Does not transport glucose (By similarity).UniProtKB/Swiss-Prot Function
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Documents
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"NM_172659" in other vectors (4)
SKU Description Size Price
MC217056 Slc2a6 (untagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 6 (Slc2a6), transcript variant 1, (10ug) 10 ug
$457.00
MG219710 Slc2a6 (tGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter) member 6 (Slc2a6) transcript variant 1, (10ug) 10 ug
$657.00
MR219710L3 Lenti ORF clone of Slc2a6 (Myc-DDK-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 6 (Slc2a6), transcript variant 1 10 ug
$757.00
MR219710L4 Lenti ORF clone of Slc2a6 (mGFP-tagged) - Mouse solute carrier family 2 (facilitated glucose transporter), member 6 (Slc2a6), transcript variant 1 10 ug
$757.00

Plasmid Map for MR219710

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219710
Circular map for MR219710

file-alt Citations

Knockout of glucose transporter GLUT6 has minimal effects on whole-body metabolic physiology in mice
Byrne, Olzomer, Brink et al
Am. J. Physiol. Endocrinol. Metab. (2018)
AI Summary: OriGene's mouse GLUT6 plasmid (MR219710 clone) was used to generate a standard curve for quantifying Slc2a6 gene copy number in mouse tissues in this study.
Snippet: 142 Quantification of Slc2a6 copy number in mouse tissues was analysed, as described (28) using a standard 143 curve prepared from mouse GLUT6 plasmid (MR219710 clone, Origene, accession number 144 NM_172659
Glucose Transporter 4 (GLUT4) is Not Necessary for Overload-Induced Glucose Uptake or Hypertrophic Growth in Mouse Skeletal Muscle
McMillin, Schmidt, Kahn et al
Diabetes (2017)
AI Summary: OriGene products, including plasmids for mouse GLUT1 (MR207871), GLUT3 (MR2097915), GLUT6 (MR219710), GLUT10 (MR227535), and a HEK293 cell GLUT10 overexpression lysate (LY410718), were used to study the expression and potential roles of alternative glucose transporters in mouse skeletal muscle during overload-induced glucose uptake.
Snippet: Plasmids containing mouse GLUT1 (catalog #MR207871), GLUT3 (catalog #MR2097915), GLUT6 (catalog #MR219710), and GLUT10 (catalog #MR227535) and an HEK293 cell GLUT10 overexpression lysate (catalog #LY410718) were purchased from OriGene;;... … Plasmids containing mouse GLUT1 (cat#MR207871), GLUT3 (cat#MR2097915), GLUT6 (cat#MR219710) and GLUT10 (cat#MR227535), and a HEK293 cell GLUT10 overexpression lysate (cat#LY410718) were purchased from Origene Technologies. ... …
Showing 1-2 of 2 papers.
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