Npffr1 (NM_001177511) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR219619
Npffr1 (Myc-DDK-tagged) - Mouse neuropeptide FF receptor 1 (Npffr1)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Npffr1
Synonyms Gm236; Gpr147; NPFF1; NPFF1R; OT7T022
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219619 representing NM_001177511
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGGAACCCTCCCAGCCTCCCAACGGCAGCTGGCCCCCGAGTCTGAACGAGAGTGATGCTGAAA
CCGCCCCGGTGGCCAGCCTCACCTTCTCTTCCTACTACCAGCACTCCTCTCCGGTGGCCGCCATGTTCAT
CGCCGCCTACGCGCTCATCTTCCTCCTCTGCATGGTGGGCAACACCCTGGTCTGCTTCATCGTGCTTAAG
AACCGGCACATGCGCACTGTCACCAACATGTTCATCCTCAACCTGGCCGTCAGCGACCTGCTGGTGGGCA
TCTTCTGCATGCCCACAACCCTCGTGGACAACCTTATCACCGGTTGGCCTTTTGACAATGCCACATGCAA
GATGAGCGGCTTGGTACAGGGCATGTCCGTGTCTGCGTCGGTTTTCACACTGGTGGCCATTGCCGTGGAG
AGGTTCCGCTGCATCGTACACCCTTTCCGTGAGAAGCTGACCCTCCGGAAGGCGCTGCTCACCATAGCGG
TGATCTGGGCGCTGGCGCTGCTCATCATGTGTCCCTCGGCGGTCACTCTGACCGTCACGCGAGAGGAGCA
TCACTTCATGCTGGATGCTCGCAACCGCTCCTACCCTCTCTACTCGTGCTGGGAGGCCTGGCCCGAGAAG
GGCATGCGCAAGGTCTACACCGCCGTGCTCTTCGCGCACATCTATCTGGCGCCGCTGGCGCTCATCGTGG
TGATGTACGCGCGCATCGCGCGCAAGCTGTGCCAGGCCCCCGGTCCAGCGCGCGACGCGGAGGAGGCGGT
GGCCGAGGGTGGCCGCGCGTCGCGCCGCAGGGCCCGCGTGGTGCATATGCTGGTCATGGTGGCGCTCTTC
TTCACCTTGTCCTGGCTGCCACTCTGGGTGCTGCTGCTGCTCATCGACTACGGGGAGCTGAGCGAGCTGC
AGCTGCACTTGCTGTCCGTCTACGCCTTCCCGTTGGCACACTGGCTGGCCTTCTTCCACAGCAGCGCCAA
CCCCATTATCTACGGCTACTTCAACGAGAACTTCCGCCGCGGCTTCCAGGCTGCCTTCCGGGCACAGCTC
TGCTGGCTTCCCTGGGCCGCCCACAAGCAAGCCTACTCCGAGCGGCCTGGCCGCCTCCTGCGCAGGCGGG
TGGTGGTGGACGTGCAACCCAGCGACTCAGGGCTGCCATCGGAGTCTGGCCCCAGCAGTGGGGTCCCAGG
GCCTAACCGGCTGCCATTGCGCAATGGGCGTGTGGCCCACCAGGATGGCCCGAGGGAAGGGCCTGGCTGC
AACCACATGCCCCTCACTATCCCAGCCTGGAACATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR219619 representing NM_001177511
Red=Cloning site Green=Tags(s)

MEAEPSQPPNGSWPPSLNESDAETAPVASLTFSSYYQHSSPVAAMFIAAYALIFLLCMVGNTLVCFIVLK
NRHMRTVTNMFILNLAVSDLLVGIFCMPTTLVDNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVE
RFRCIVHPFREKLTLRKALLTIAVIWALALLIMCPSAVTLTVTREEHHFMLDARNRSYPLYSCWEAWPEK
GMRKVYTAVLFAHIYLAPLALIVVMYARIARKLCQAPGPARDAEEAVAEGGRASRRRARVVHMLVMVALF
FTLSWLPLWVLLLLIDYGELSELQLHLLSVYAFPLAHWLAFFHSSANPIIYGYFNENFRRGFQAAFRAQL
CWLPWAAHKQAYSERPGRLLRRRVVVDVQPSDSGLPSESGPSSGVPGPNRLPLRNGRVAHQDGPREGPGC
NHMPLTIPAWNI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001177511
ORF Size 1296 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001177511.2
RefSeq Size 1307 bp
RefSeq ORF 1299 bp
Locus ID 237362
Cytogenetics 10 B4
MW 48.7 kDa
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Documents
Resources
"NM_001177511" in other vectors (4)
SKU Description Size Price
MC213038 Npffr1 (untagged) - Mouse neuropeptide FF receptor 1 (Npffr1), (10ug) 10 ug
$686.00
MG219619 Npffr1 (tGFP-tagged) - Mouse neuropeptide FF receptor 1 (Npffr1), (10ug) 10 ug
$886.00
MR219619L3 Lenti ORF clone of Npffr1 (Myc-DDK-tagged) - Mouse neuropeptide FF receptor 1 (Npffr1) 10 ug
$986.00
MR219619L4 Lenti ORF clone of Npffr1 (mGFP-tagged) - Mouse neuropeptide FF receptor 1 (Npffr1) 10 ug
$986.00

Plasmid Map for MR219619

Download sequence for

Cloning scheme for MR219619

Circular map for MR219619

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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