Rapgef2 (NM_001099624) Mouse Tagged ORF Clone

SKU MR219556

Rapgef2 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF) 2 (Rapgef2)

Product Data

• Type: Mouse Tagged ORF Clone
• Target Symbol: Rapgef2
• Synonyms: 5830453M24Rik; CNRasGEF; mKIAA0313; nRapGEP; Pdzgef1; RA-GEF-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >MR219556 representing NM_001099624; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCTTTCCTTGTGCGTTGCTATGCCAACTGCCTGCAGCCATGGTCCTCCAAACTCCCTGCAGACTTCA; CAAAACTGCACCTCACTGACAGCCTGCACCCACAGGTGACCCACGTGTCCTCCAGCCATTCAGGATGTAG; CATCACCAGCGACTCTGGGAGCAGCAGTCTTTCTGACATTTACCAGGCCACGGAGAGCGAGGCCGGTGAC; ATGGACCTGAGTGGACTGCCAGAGACGGCGGTTGACTCTGAGGATGACGATGATGAGGAAGACATCGAGA; GAGCATCAGACCCTCTGATGAGCAGGGACATCGTGAGGGACTGCCTGGAGAAGGACCCGATAGACAGGAC; TGACGATGACATCGAACAACTACTGGAATTTATGCACCAGCTGCCGGCCTTTGCCAATATGACGATGTCA; GTAAGACGGGAGCTCTGTGCCGTGATGGTGTTTGCAGTGGTGGAAAGAGCTGGAACCATTGTGTTAAACG; ACGGAGAAGAGCTGGATTCCTGGTCAGTGATTCTCAATGGTTCCGTGGAGGTGACATATCCGGATGGGAA; AGCAGAAATTCTGTGCATGGGAAACAGCTTTGGTGTTTCGCCCACCATGGACAAAGAATACATGAAAGGA; GTCATGAGGACTAAGGTGGATGACTGCCAGTTTGTCTGCATTGCCCAGCAAGATTACTGCCGTATTTTGA; ACCAAGTAGAAAAGAACATGCAAAAAGTTGAGGAGGAAGGAGAGATTGTAATGGTGAAGGAGCACCGAGA; ACTTGATCGGACTGGAACAAGGAAGGGCCACATCGTCATCAAGGGCACCTCAGAGAGGCTGACCATGCAT; TTGGTGGAAGAGCATTCTGTAGTGGATCCAACATTCATAGAAGACTTCCTGTTGACATACCGGACTTTCC; TTTCCAGCCCGATGGAAGTGGGCAAGAAGTTATTGGAGTGGTTCAATGACCCGAGCCTCAGGGATAAGGT; GACGCGGGTAGTATTACTGTGGGTGAATAATCACTTCAATGACTTTGAAGGGGATCCTGCAATGACTCGA; TTTCTGGAAGAATTTGAAAATAATCTGGAAAGAGAGAAAATGGGTGGACATCTAAGGCTGTTGAACATTG; CATGTGCAGCTAAAGCCAAACGAAGACTGATGACGTTAACAAAGCCTTCCCGGGAAGCCCCTCTGCCCTT; CATCTTACTGGGAGGCTCTGAAAAGGGCTTTGGCATCTTCGTGGACAGCGTTGACTCGTGTAGCAAAGCC; ACTGAAGCAGGCTTGAAGCGCGGGGATCAGATATTAGAAGTGAATGGTCAAAATTTTGAAAATATCCAAC; TTTCAAAGGCAATGGAAATTCTCAGAAATAACACACACCTGTCCATCACTGTGAAAACCAACTTATTTGT; ATTTAAAGAACTTCTGACGAGATTGTCTGAAGAGAAAAGAAACGGTGCCCCTCACCTCCCTAAAATCGGG; GACATCAAGAAGGCCAGCCGCTACTCCATACCGGATCTCGCCGTAGACGTAGAACAGGTGATAGGCCTCG; AGAAGGTCAACAAGAAAAGCAAAGCCAACACCGTGGGAGGAAGAAACAAGCTGAAGAAAATACTCGACAA; GACCCGGATCAGCATCTTACCTCAGAAACCATACAATGATATTGGGATTGGTCAGTCTCAAGATGATAGC; ATCGTAGGACTGAGGCAGACCAAGCACATCCCAGCTGCCCTGCCTGTCAGTGGAACGCTGTCCTCCAGTA; ACCCTGACTTGCTGCAGTCACACCACCGGATTCTGGACTTCAGCACTACTCCTGACTTGCCAGATCAGGT; ACTGAGAGTTTTCAAGGCTGACCAACAAAGCAGGTACATCATGATCAGCAAGGACACCACCGCAAAGGAA; GTGGTCATCCAGGCCATCAGAGAGTTTGCTGTCACTGCCACCCCAGAGCAGTATTCCCTCTGTGAGGTCT; CAGTCACACCAGAGGGAGTTATCAAACAACGCCGACTCCCAGATCAGCTTTCCAAGCTTGCCGACAGGAT; ACAACTGAGTGGAAGGTATTATCTGAAAAACAACATGGAAACAGAAACGCTTTGCTCAGATGAAGATGCC; CAGGAGCTGCTGCGAGAGAGCCAAATCTCCCTCCTTCAGCTCAGCACCGTGGAGGTTGCCACGCAGCTCT; CCATGAGGAACTTTGAACTCTTCCGCAACATTGAGCCTACTGAATACATAGATGATTTATTTAAACTCAA; ATCAAAAACCAGCTGTGCCAACCTGAAGAAATTTGAGGAAGTCATTAACCAGGAAACATTTTGGGTGGCT; TCTGAAATCCTGCGGGAAACAAACCAGCTGAAGAGGATGAAGATCATTAAGCATTTCATCAAGATTGCCT; TGCACTGCAGGGAATGCAAGAATTTTAACTCCATGTTTGCAATCATCAGTGGCCTAAACCTGGCACCGGT; GGCAAGACTACGAACCACCTGGGAAAAGCTTCCCAATAAATATGAAAAGCTGTTCCAGGACCTGCAAGAC; CTGTTTGATCCTTCCAGAAACATGGCAAAGTACCGCAATGTCCTGAGTGGCCAGAACTTACAGCCTCCTG; TCATTCCCCTGTTCCCTGTCATCAAGAAGGACCTCACGTTTCTCCATGAGGGAAATGACTCAAAAGTAGA; CGGACTGGTCAACTTTGAGAAGCTGAGGATGATTGCAAAAGAAATCCGTCACGTTGGCCGAATGGCATCA; GTCAACATGGACCCTGCCCTTATGTTCAGGACTCGGAAGAAGAAGTGGCGGAGTCTGGGGTCCCTGAGTC; AGGGCAGTGCAAACGCCACAGTGCTGGATGTTGCTCAGACAGGCGGGCATAAAAAGCGGGTACGCCGTAG; TTCCTTCCTCAATGCCAAAAAGCTGTATGAAGATGCCCAGATGGCTCGGAAAGTGAAGCAATACCTGTCC; AACCTGGAGCTGGAGATGGATGAGGAGAGTCTTCAGACACTGTCACTGCAGTGTGAACCAGCAACCAGCA; CATTGCCCAAGAATCCTGGAGACAAAAAGCCTGTCAAGTCTGAGACCTCCCCAGTGGCTCCACGTGCAGG; GCCCCAGCAGAAAGTACAGCCTCAGCAGCCGCTGGCACAGCCACAACCTCCACATAAAGTCAGCCAGGGA; CTGCAGGTGCCCGCTGTGTCCCTCTATCCTTCACGAAAGAAGGTGCCGGTGAAGGATCTGCCTCCTTTTG; GCATAAACTCTCCACAAGCTTTGAAGAAAATCCTGTCCTTGTCTGAAGAAGGGAGTTTGGAGCGTCACAG; GAAGCAAGCAGAAGATACAATATCAAATGCGTCCTCTCAGCTCTCATCGCCCCCCACCTCACCTCAGAGC; TCTCCAAGGAAAGGTTATGCACTGGCTCTCAGTGGCACCGTGGATAATTTCTCAGATTCAGGTCACAGTG; AGATTTCTTCACGATCCAGTATTGTCAGCAATTCTTCTTTTGACTCGGTGCCAGTCTCTCTGCATGATGA; ACGGCGCCAGAGGCATTCTGTCAGCATTGTGGAGAGCAACCTAGGTGTGGGCAGGATGGAGCGGCGGACC; CTGATGGAGCCTGATCAATACAGCTTAGGGTCGTATGCCCCAGTGTCTGAAAGCCGAGGCCTGTATGCCG; CAGCGACGGTCATCTCTTCTCCTAGCACAGAGGAGCTTTCCCATGACCAAGGGGACCGTGCTTCCCTCGA; TGCTGCTGACAGCGGTCGTGGGAGCTGGACATCATGCTCAAGTGGTTCCCATGACAACATACAGACCATT; CAGCACCAGAGAAGCTGGGAAACACTGCCATTTGGGCACACTCACTTTGACTATTCAGGGGATGCTGCAA; GTATATGGGCCTCCGGTGGCCATATGGACCAAATGATGTTTTCTGATCATAGCACAAAATATAACAGACA; GAATCAAAGTAGAGAGAGTCTTGAACAAGCCCAATCCCGGGCCAGCTGGGCGTCTTCCACGGGCTACTGG; GGAGAAGACTCAGAAGGCGACACAGGCACTATCAAGCGAAGGGGTGGGAAGGATGTCTCCGCTGAGGCAG; AGAGCAGCAGCATGGTGCCCGTGACTACAGAGGAAGCCAAACCTGTCCCTATGCCTGCCCACATAGCTGT; GACGCCGAGCACTACCAAGGGACTCATCGCACGGAAGGAAGGCAGGTACCGGGAGCCGCCTCCCACACCT; CCAGGCTACGTGGGCATCCCCATTGCCGATTTCCCAGAAGGGCCTTGCCACCCGGCCAGGAAGCCCCCGG; ATTACAACGTGGCCCTGCAGCGGTCCCGCATGGTGGCACGGCCCACTGAGGCCCCGGCACCGGGCCAGAC; GCCGCCTGCAGCCGCAGCCAGCCGGCCGGGCAGCAAGCCACAGTGGCACAAGCCCAGCGACGCAGACCCA; CGCCTCGCGCCCTTCCAGCCGCAGGGCTTCGCAGGAGCGGAGGAGGACGAAGATGAACAAGTGTCTGCTG; TT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >MR219556 representing NM_001099624; Red=Cloning site Green=Tags(s); ; MAFLVRCYANCLQPWSSKLPADFTKLHLTDSLHPQVTHVSSSHSGCSITSDSGSSSLSDIYQATESEAGD; MDLSGLPETAVDSEDDDDEEDIERASDPLMSRDIVRDCLEKDPIDRTDDDIEQLLEFMHQLPAFANMTMS; VRRELCAVMVFAVVERAGTIVLNDGEELDSWSVILNGSVEVTYPDGKAEILCMGNSFGVSPTMDKEYMKG; VMRTKVDDCQFVCIAQQDYCRILNQVEKNMQKVEEEGEIVMVKEHRELDRTGTRKGHIVIKGTSERLTMH; LVEEHSVVDPTFIEDFLLTYRTFLSSPMEVGKKLLEWFNDPSLRDKVTRVVLLWVNNHFNDFEGDPAMTR; FLEEFENNLEREKMGGHLRLLNIACAAKAKRRLMTLTKPSREAPLPFILLGGSEKGFGIFVDSVDSCSKA; TEAGLKRGDQILEVNGQNFENIQLSKAMEILRNNTHLSITVKTNLFVFKELLTRLSEEKRNGAPHLPKIG; DIKKASRYSIPDLAVDVEQVIGLEKVNKKSKANTVGGRNKLKKILDKTRISILPQKPYNDIGIGQSQDDS; IVGLRQTKHIPAALPVSGTLSSSNPDLLQSHHRILDFSTTPDLPDQVLRVFKADQQSRYIMISKDTTAKE; VVIQAIREFAVTATPEQYSLCEVSVTPEGVIKQRRLPDQLSKLADRIQLSGRYYLKNNMETETLCSDEDA; QELLRESQISLLQLSTVEVATQLSMRNFELFRNIEPTEYIDDLFKLKSKTSCANLKKFEEVINQETFWVA; SEILRETNQLKRMKIIKHFIKIALHCRECKNFNSMFAIISGLNLAPVARLRTTWEKLPNKYEKLFQDLQD; LFDPSRNMAKYRNVLSGQNLQPPVIPLFPVIKKDLTFLHEGNDSKVDGLVNFEKLRMIAKEIRHVGRMAS; VNMDPALMFRTRKKKWRSLGSLSQGSANATVLDVAQTGGHKKRVRRSSFLNAKKLYEDAQMARKVKQYLS; NLELEMDEESLQTLSLQCEPATSTLPKNPGDKKPVKSETSPVAPRAGPQQKVQPQQPLAQPQPPHKVSQG; LQVPAVSLYPSRKKVPVKDLPPFGINSPQALKKILSLSEEGSLERHRKQAEDTISNASSQLSSPPTSPQS; SPRKGYALALSGTVDNFSDSGHSEISSRSSIVSNSSFDSVPVSLHDERRQRHSVSIVESNLGVGRMERRT; LMEPDQYSLGSYAPVSESRGLYAAATVISSPSTEELSHDQGDRASLDAADSGRGSWTSCSSGSHDNIQTI; QHQRSWETLPFGHTHFDYSGDAASIWASGGHMDQMMFSDHSTKYNRQNQSRESLEQAQSRASWASSTGYW; GEDSEGDTGTIKRRGGKDVSAEAESSSMVPVTTEEAKPVPMPAHIAVTPSTTKGLIARKEGRYREPPPTP; PGYVGIPIADFPEGPCHPARKPPDYNVALQRSRMVARPTEAPAPGQTPPAAAASRPGSKPQWHKPSDADP; RLAPFQPQGFAGAEEDEDEQVSAV; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001099624
• ORF Size: 4482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001099624.3, NP_001093094.2
• RefSeq Size: 6583 bp
• RefSeq ORF: 4485 bp
• Locus ID: 76089
• Cytogenetics: 3 E3
• MW: 166.4 kDa
• Summary: Functions as a guanine nucleotide exchange factor (GEF), which activates Rap and Ras family of small GTPases by exchanging bound GDP for free GTP in a cAMP-dependent manner. Serves as a link between cell surface receptors and Rap/Ras GTPases in intracellular signaling cascades. Acts also as an effector for Rap1 by direct association with Rap1-GTP thereby leading to the amplification of Rap1-mediated signaling. Shows weak activity on HRAS. It is controversial whether RAPGEF2 binds cAMP and cGMP or not. Its binding to ligand-activated beta-1 adrenergic receptor ADRB1 leads to the Ras activation through the G(s)-alpha signaling pathway. Involved in the cAMP-induced Ras and Erk1/2 signaling pathway that leads to sustained inhibition of long term melanogenesis by reducing dendrite extension and melanin synthesis. Provides also inhibitory signals for cell proliferation of melanoma cells and promotes their apoptosis in a cAMP-independent nanner. Regulates cAMP-induced neuritogenesis by mediating the Rap1/B-Raf/ERK signaling through a pathway that is independent on both PKA and RAPGEF3/RAPGEF4. Involved in neuron migration and in the formation of the major forebrain fiber connections forming the corpus callosum, the anterior commissure and the hippocampal commissure during brain development. Involved in neuronal growth factor (NGF)-induced sustained activation of Rap1 at late endosomes and in brain-derived neurotrophic factor (BDNF)-induced axon outgrowth of hippocampal neurons. Plays a role in the regulation of embryonic blood vessel formation and in the establishment of basal junction integrity and endothelial barrier function. May be involved in the regulation of the vascular endothelial growth factor receptor KDR and cadherin CDH5 expression at allantois endothelial cell-cell junctions.[UniProtKB/Swiss-Prot Function]