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Pde7a (NM_008802) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219166
Pde7a (Myc-DDK-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $503.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pde7a
Synonyms AU015378; AW047537
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219166 representing NM_008802
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAATCACTTTGATCTGGTGTCTGGCTTTGGTTCTGATCAAGTGGATCACCTCTAAGAGACGTGGAG
CTATTTCCTATGACAGTTCTGATCAGACTGCGTTATATATTCGTATGCTAGGAGATGTCCGCGTGAGGAG
CCGAGCAGGATTTGAAACAGAAAGAAGAGGTTCCCATCCGTACATTGACTTCCGTATTTTTCACTCTCAA
TCTGACATTGAAGCATCAGTCTCCGCCAGGAACATCAGAAGGTTACTAAGTTTCCAGCGATATCTCAGAT
CCTCACGAGTTTTTCGGGGTGCCACAGTTTGTAGTTCTCTAGACATTTTAGATGAGGATTACAATGGACA
AGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGATATCTTTCTGTTTGATAGACTAACAAAT
GGAAATAGTCTAGTAAGCCTAACCTTTCATTTATTTAGTCTTCATGGATTGATTGAGTACTTCCATTTAG
ATATGGTGAAACTCCGTCGGTTTTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCTTACCACAA
TGCAGTCCATGCTGCAGACGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTGCCAGTTCT
GTCACTCCTTGGGATATCTTGCTGAGTTTAATTGCAGCCGCCACTCACGATCTGGATCACCCAGGTGTTA
ATCAGCCGTTTCTTATTAAAACCAACCATTATCTAGCAACTTTATACAAGAATTCCTCAGTCCTGGAGAA
TCACCACTGGAGATCTGCAGTGGGCTTGTTAAGAGAATCTGGTTTGTTCTCACACTTGCCATTGGAAAGC
AGGCAAGAGATGGAGGCTCAGATAGGTGCTTTGATATTAGCCACGGATATCAGTCGCCAGAACGAGTACC
TGTCATTGTTTAGATCTCACTTGGATAAAGGTGACTTACACCTTGACGATGGCAGACATAGGCATTTGGT
TCTACAGATGGCCTTGAAATGTGCTGATATTTGTAACCCATGTCGGAACTGGGAATTAAGCAAGCAGTGG
AGTGAAAAAGTAACGGAGGAATTCTTCCACCAAGGAGATATAGAAAAAAAGTACCATTTGGGTGTGAGTC
CACTTTGTGATCGTCAGACTGAGTCTATTGCCAACATCCAGATTGGTTTTATGACTTACCTAGTGGAGCC
TTTATTTACAGAGTGGGCCAGGTTTTCAGACACACGGCTGTCACAGACGATGCTTGGACATGTGGGGCTG
AATAAAGCCAGCTGGAAGGGACTGCAGAGACAACAGCCCAGCAGCGAGGATGCCAATGCTGCATTTGAGT
TGAACTCACAGTTACTAACTCAGGAAAATCGGTTATCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219166 representing NM_008802
Red=Cloning site Green=Tags(s)

MGITLIWCLALVLIKWITSKRRGAISYDSSDQTALYIRMLGDVRVRSRAGFETERRGSHPYIDFRIFHSQ
SDIEASVSARNIRRLLSFQRYLRSSRVFRGATVCSSLDILDEDYNGQAKCMLEKVGNWNFDIFLFDRLTN
GNSLVSLTFHLFSLHGLIEYFHLDMVKLRRFLVMIQEDYHSQNPYHNAVHAADVTQAMHCYLKEPKLASS
VTPWDILLSLIAAATHDLDHPGVNQPFLIKTNHYLATLYKNSSVLENHHWRSAVGLLRESGLFSHLPLES
RQEMEAQIGALILATDISRQNEYLSLFRSHLDKGDLHLDDGRHRHLVLQMALKCADICNPCRNWELSKQW
SEKVTEEFFHQGDIEKKYHLGVSPLCDRQTESIANIQIGFMTYLVEPLFTEWARFSDTRLSQTMLGHVGL
NKASWKGLQRQQPSSEDANAAFELNSQLLTQENRLS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008802
ORF Size 1368 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008802.3, NP_032828.2
RefSeq Size 3179 bp
RefSeq ORF 1371 bp
Locus ID 18583
UniProt ID P70453
Cytogenetics 3 5.36 cM
MW 52.9 kDa
Summary Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May have a role in muscle signal transduction (By similarity).UniProtKB/Swiss-Prot Function
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"NM_008802" in other vectors (6)
SKU Description Size Price
MC216126 Pde7a (untagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 1, (10ug) 10 ug
$503.00
MG219166 Pde7a (tGFP-tagged) - Mouse phosphodiesterase 7A (Pde7a) transcript variant 1, (10ug) 10 ug
$703.00
MR219166L1 Lenti ORF clone of Pde7a (Myc-DDK-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 1 10 ug
$803.00
MR219166L2 Lenti ORF clone of Pde7a (mGFP-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 1 10 ug
$803.00
MR219166L3 Lenti ORF clone of Pde7a (Myc-DDK-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 1 10 ug
$803.00
MR219166L4 Lenti ORF clone of Pde7a (mGFP-tagged) - Mouse phosphodiesterase 7A (Pde7a), transcript variant 1 10 ug
$803.00

Plasmid Map for MR219166

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219166
Circular map for MR219166

file-alt Citations

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