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Armc8 (NM_028768) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219078
Armc8 (Myc-DDK-tagged) - Mouse armadillo repeat containing 8 (Armc8), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $616.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Armc8
Synonyms 1200015K23Rik; Gid5; HSPC056
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219078 representing NM_028768
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTGCCTGTTGGAGACCCCAATCCGCATGAGCGTCCTCTCGGAGGTAACAGCTAGCAGTCGCCACT
ATGTTGACAGGCTATTTGACCCTGATCCCCAGAAAGTTCTCCAAGGTGTCATAGACATGAAAAATGCTGT
AATTGGAAACAATAAACAGAAAGCCAACCTCATCGTTCTAGGAGCGGTTCCAAGATTGTTGTATTTGCTT
CAACAAGAAACCTCAAGTACAGAGCTGAAAACTGAATGTGCAGTGGTGTTAGGGAGTCTTGCGATGGGCA
CAGAAAACAATGTCAAGTCTCTACTAGACTGCCATATTATCCCTGCCTTACTACAAGGACTACTGTCCCC
AGACCTGAAGTTCATTGAAGCTTGCCTCCGATGCCTCCGGACAATCTTCACCAGCCCTGTCACTCCAGAG
GAGCTACTGTATACAGATGCTACGGTGATACCACACCTCATGGCACTGCTGAGCAGGTCCCGCTACACCC
AGGAGTATATCTGCCAGATCTTCTCACACTGTTGCAAAGGGCCAGATCATCAAACAATTTTGTTTAACCA
TGGGGCAGTGCAGAACATTGCTCATCTCCTAACCTCACCCTCTTACAAAGTTCGAATGCAAGCACTGAAA
TGCTTCTCTGTTTTAGCCTTTGAGAATCCCCAAGTATCGATGACCCTGGTAAACGTTTTGGTTGATGGGG
AGTTGTTACCACAGATATTTGTGAAGATGTTACAGAGGGATAAGCCTATTGAGATGCAGCTCACGTCAGC
AAAATGTTTAACTTACATGTGCAGAGCTGGAGCAATTCGCACAGATGACAGCTGTATTGTATTAAAAACT
TTGCCTTGTTTGGTTCGAATGTGCAGTAAGGAGAGGTTACTAGAGGAGAGAGTTGAAGGAGCTGAGACCC
TCGCCTATCTGATTGAGCCAGATGTTGAGCTGCAGAGAATCGCTAGCATAACCGACCACCTCATTGCCAT
GCTGGCTGACTATTTCAAGTATCCCAGCTCAGTGAGCGCCATCACTGACATTAAAAGGCTTGATCATGAC
TTAAAACATGCCCATGAGCTCCGCCAGGCTGCGTTCAAGCTCTATGCCTCTCTTGGAGCAAATGATGAAG
ACATCCGGAAGAAGATCATTGAGACTGAAACTATGATGGACCGAATTGTGACTGGCTTGTCTGAGTCTAG
TGTCAAGGTGCGGTTAGCTGCTGTCAGATGTTTGCACAGTTTATCCAGATCTGTACAACAGCTTCGAACC
AGTTTCCAGGATCATGCGGTGTGGAAACCTTTAATGAAGGTTTTACAAAATGCACCCGATGAAATCCTAG
TAGTAGCATCTTCCATGTTATGTAACCTTCTTCTTGAATTTTCTCCAAGTAAAGAGCCAATTTTGGAATC
AGGAGCTGTCGAGCTGCTCTGTGGGTTGACACAGAGTGAAAACCCTGCTCTACGAGTGAATGGGATTTGG
GCTTTAATGAATATGGCATTTCAGGCGGAACAAAAAATAAAAGCAGATATTTTACGAAGTCTGAGTACTG
AACAGTTATTCCGGTTATTGTCAGATTCTGATATGAATGTGCTGATGAAGACATTGGGGCTTCTTAGAAA
TCTCCTCTCCACTCGGCCTCATATAGATAAAATCATGAGTACTCATGGGAAGCAGATTATGCAAGCTGTC
ACTCTGATACTGGAAGGGGAGCATAGCATTGAAGTCAAAGAGCAGACCCTGTGCATCCTAGCCAACATTG
CAGATGGGACAACAGCAAAAGAGCTTATTATGACCAATGATGACATCCTGCAGAAAATCAAATATTACAT
GGGTCATTCACATGTCAAACTGCAGCTTGCTGCCATGTTTTGTATATCAAACCTCATATGGAACGAAGAG
GAAGGTTCACAGGAACGCCAGGATAAATTACGAGACATGGGGATTGTAGATATTCTACACAAACTGAGTC
AGTCGGCAGACTCAAACCTCTGTGACAAGGCAAAGACGGCGCTGCAGCAGTACCTGGCG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219078 representing NM_028768
Red=Cloning site Green=Tags(s)

MACLLETPIRMSVLSEVTASSRHYVDRLFDPDPQKVLQGVIDMKNAVIGNNKQKANLIVLGAVPRLLYLL
QQETSSTELKTECAVVLGSLAMGTENNVKSLLDCHIIPALLQGLLSPDLKFIEACLRCLRTIFTSPVTPE
ELLYTDATVIPHLMALLSRSRYTQEYICQIFSHCCKGPDHQTILFNHGAVQNIAHLLTSPSYKVRMQALK
CFSVLAFENPQVSMTLVNVLVDGELLPQIFVKMLQRDKPIEMQLTSAKCLTYMCRAGAIRTDDSCIVLKT
LPCLVRMCSKERLLEERVEGAETLAYLIEPDVELQRIASITDHLIAMLADYFKYPSSVSAITDIKRLDHD
LKHAHELRQAAFKLYASLGANDEDIRKKIIETETMMDRIVTGLSESSVKVRLAAVRCLHSLSRSVQQLRT
SFQDHAVWKPLMKVLQNAPDEILVVASSMLCNLLLEFSPSKEPILESGAVELLCGLTQSENPALRVNGIW
ALMNMAFQAEQKIKADILRSLSTEQLFRLLSDSDMNVLMKTLGLLRNLLSTRPHIDKIMSTHGKQIMQAV
TLILEGEHSIEVKEQTLCILANIADGTTAKELIMTNDDILQKIKYYMGHSHVKLQLAAMFCISNLIWNEE
EGSQERQDKLRDMGIVDILHKLSQSADSNLCDKAKTALQQYLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_028768
ORF Size 2019 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_028768.3, NP_083044.2
RefSeq Size 4628 bp
RefSeq ORF 2022 bp
Locus ID 74125
UniProt ID Q9DBR3
Cytogenetics 9 E3.3
MW 75.4 kDa
Summary Component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1.UniProtKB/Swiss-Prot Function
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Documents
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"NM_028768" in other vectors (4)
SKU Description Size Price
MC220356 Armc8 (untagged) - Mouse armadillo repeat containing 8 (Armc8), transcript variant 1, (10ug) 10 ug
$679.00
MG219078 Armc8 (tGFP-tagged) - Mouse armadillo repeat containing 8 (Armc8) transcript variant 1, (10ug) 10 ug
$816.00
MR219078L3 Lenti ORF clone of Armc8 (Myc-DDK-tagged) - Mouse armadillo repeat containing 8 (Armc8), transcript variant 1 10 ug
$916.00
MR219078L4 Lenti ORF clone of Armc8 (mGFP-tagged) - Mouse armadillo repeat containing 8 (Armc8), transcript variant 1 10 ug
$916.00

Plasmid Map for MR219078

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219078
Circular map for MR219078

file-alt Citations

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