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Cct6b (NM_009839) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR219047
Cct6b (Myc-DDK-tagged) - Mouse chaperonin containing Tcp1, subunit 6b (zeta) (Cct6b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $494.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cct6b
Synonyms CCT-zeta-2; Cctz-2; CCTzeta-2; TCP-1-zeta-2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR219047 representing NM_009839
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCGATCAAAATCGCTAACCCTGGCGCTGAAGTGACCCGGAGCCAAGCGGCCTTGGCTGTGAACA
TCTGCGCCGCCCGTGGGCTGCAGGATGTGCTGCGTCCCACCTTGGGTCCTAAGGGCGCGCTAAAAATGCT
TGTTTCTGGGGCAGGTGACATCAAACTCACCAAAGACGGCAATGTACTGCTTCATGAGATGCAAATTCAA
CACCCAACGGCTTCCATCATTGCAAAAGTGGCAGCAGCCCAAGACCATGTCACAGGGGATGGCACTACCT
CAAATGTCCTGATCATTGGAGAGTTGCTAAAGCAAGCTGATCTTTACATTTCTGAGGGCCTGCACCCTAG
AATCATAACTGAAGGATTTGATGTTGCAAAGACGAAAGCACTTGAAGTTTTGGATGAAATTAAAGTACAA
AAGGAGATGAAAAGAGAAATCCTCTTGGATGTGGCTAGAACATCCCTGCAGACGAAAGTTCATGCTGAAC
TGGCCGACATACTAACAGAGGCTGTGGTGGATTCTGTCTTGGCTATCAGAAGACCAGGTGTCCCTATTGA
TCTCTTCATGGTGGAAATTGTGGAGATGAGGCACAAGTCAGAAACGGACACACAGTTGATCCGAGGATTG
GTTTTGGACCATGGTGCCCGTCATCCAAGGATGAGGAAGCAGGTGCGAGATGCATACATCCTCACTTGCA
ATGTCTCACTAGAGTATGAAAAAACAGAAGTGAGCTCTGGTTTCTTTTATAAGACTGTAGAAGAGAAAGA
GAAATTGGTAAAAGCTGAAAGAAAATTTATTGAAGATAGAGTGCAGAAAATAATAGACCTAAAGCAGAAA
GTCTGTGCTGAGTCCAATAAAGGATTTGTTGTCATTAATCAAAAGGGCATCGATCCGGTTTCCTTAGAAA
TGCTTGCAAAACATAACATCGTAGCTCTTCGCAGGGCAAAAAGAAGGAATCTTGAAAGGCTCACCCTTGC
TTGTGGAGGATTGGCTGTCAACTCCTTTGAAGGCCTGAGTGAAGAGTGCCTGGGGCACGCTGGCCTTGTC
TTCGAGTACGCATTGGGTGAAGAAAAGTTCACTTTTATTGAGGACTGTGTAAATCCGCTCTCTGTCACCT
TGTTGGTCAAAGGGCCAAATAAGCATACTCTCATACAGATCAAGGACGCCTTAAGAGATGGACTCCGTGC
GGTCAAAAATGCTATTGAAGACGGCTGTGTGGTTCCAGGGGCTGGGGCAGTGGAAGTTGCGATAGCAGAA
GCTCTTGTTAACTACAAGCACCGTGTGCAGGGAAGAGTTCGCCTGGGGATCCAAGCCTTTGCTGATGCCT
TGCTCATCATCCCGAAGGTTCTTGCTCAGAATTCTGGTTATGACCTGCAGGAAACACTGATAAAAATTCA
AACTAAACATGCCGAATCAAAAGAACTCCTGGGCATAGACCTGAACACAGGGGAGCCGATGGCAGCAGCA
GAAGCAGGAATCTGGGATAATTACTGTGTGAAAAAACATCTTCTTCACTCTTGCACGGTGATTGCCACCA
ACATCCTCCTGGTTGATGAAATTATGCGAGCTGGGATGTCTTCTCTTAGAGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR219047 representing NM_009839
Red=Cloning site Green=Tags(s)

MAAIKIANPGAEVTRSQAALAVNICAARGLQDVLRPTLGPKGALKMLVSGAGDIKLTKDGNVLLHEMQIQ
HPTASIIAKVAAAQDHVTGDGTTSNVLIIGELLKQADLYISEGLHPRIITEGFDVAKTKALEVLDEIKVQ
KEMKREILLDVARTSLQTKVHAELADILTEAVVDSVLAIRRPGVPIDLFMVEIVEMRHKSETDTQLIRGL
VLDHGARHPRMRKQVRDAYILTCNVSLEYEKTEVSSGFFYKTVEEKEKLVKAERKFIEDRVQKIIDLKQK
VCAESNKGFVVINQKGIDPVSLEMLAKHNIVALRRAKRRNLERLTLACGGLAVNSFEGLSEECLGHAGLV
FEYALGEEKFTFIEDCVNPLSVTLLVKGPNKHTLIQIKDALRDGLRAVKNAIEDGCVVPGAGAVEVAIAE
ALVNYKHRVQGRVRLGIQAFADALLIIPKVLAQNSGYDLQETLIKIQTKHAESKELLGIDLNTGEPMAAA
EAGIWDNYCVKKHLLHSCTVIATNILLVDEIMRAGMSSLRD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009839
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009839.3, NP_033969.2
RefSeq Size 1817 bp
RefSeq ORF 1596 bp
Locus ID 12467
UniProt ID Q61390
Cytogenetics 11 C
MW 58.2 kDa
Summary Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis.UniProtKB/Swiss-Prot Function
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Documents
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"NM_009839" in other vectors (4)
SKU Description Size Price
MC217559 Cct6b (untagged) - Mouse chaperonin containing Tcp1, subunit 6b (zeta) (Cct6b), (10ug) 10 ug
$545.00
MG219047 Cct6b (tGFP-tagged) - Mouse chaperonin containing Tcp1 subunit 6b (zeta) (Cct6b), (10ug) 10 ug
$694.00
MR219047L3 Lenti ORF clone of Cct6b (Myc-DDK-tagged) - Mouse chaperonin containing Tcp1, subunit 6b (zeta) (Cct6b) 10 ug
$794.00
MR219047L4 Lenti ORF clone of Cct6b (mGFP-tagged) - Mouse chaperonin containing Tcp1, subunit 6b (zeta) (Cct6b) 10 ug
$794.00

Plasmid Map for MR219047

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR219047
Circular map for MR219047

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