Pitpnm3 (NM_001024927) Mouse Tagged ORF Clone

SKU
MR218936
Pitpnm3 (Myc-DDK-tagged) - Mouse PITPNM family member 3 (Pitpnm3), transcript variant 1
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$589.00 MSRP $892.00 MSRP $892.00
In Stock*
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pitpnm3
Synonyms A330068P14Rik; Ackr6; AI848332; Gm880
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218936 representing NM_001024927
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAGGCGGGCAGCGCAGGTGGCCCTTCCCCGGGTGGCGGTGCCCCCTGGCACCTTCGGAATGTCC
TCAGTGATTCGGTGGAGAGTTCAGATGATGAATTCTTTGATGCTAGAGAGGAGGTGGCTGAAGGAAAGAA
CGCCATCCTTATTGGAATGAGCCAGTGGAGCTCCAATGACCTGGTAGAGCAGATCGAGACCATAGGGAAG
CTGGATGAGCGTCAGGGAGACGGGGCCACAGCGTGCACATCCAGCATCCTGCAGGAGAAGCAGCGAGAAC
TGTACCGGGTGTCCCTCAGAAGGCAGAGGTTCCCAGCCCAAGGAAGTATCGAGATTCATGAAGATGGCGA
GGAAGGCTGCTCACAGCGCTCCTGCAAGACACACGTCCTTCTGCTGGTGCTGCATGGGGGCAACGTCCTG
GACACCGGCTCTGGTGACCCTTCCTGCAAGGCGGCCGACATTCACACTTTCAGCTCTGTGCTGGAGAAGG
TCATGCGCGCCCACTTCCCTGCTGCCCTGGGACACATCCTCATCAAGTTTGTCCCTTGTCCTGCCATCTG
CTCGGAGGCTTTCTCGCTCGTCTCTAACCTGAACCCCTATAGCCACGACGAGGGCTGCCTCGGTACCAGC
CAGGACCACGTGCCCCTGGCTGCCCTTCCCCTGCTGGCCATCTCCTCCCCACAGTACCAGGACGCTGTTG
CCACTGTCATCGAGCGAGCCAACCACATCTATGGCGAGTTCCTCAAGTCCTCTGATGGGATTGGCTTCAA
TGGGCAGGTGTGTCTCATCGGGGACTGTGTGGGCGGCCTCCTGGCTTTCGATGCTATCTGCTACAGTGCG
GGACCCTCAGGGGACAGCCCTGGCAGCAGCAGCCGGAAGGGGAGCATCAGCAGTACCCAGGACACCCCTG
TGGTGGTGGAAGAGGACTGCAGTCTGGCCAGCAGCAAGCGGCTCAGTAAGAGCAATGTCGATGTCTCCAG
TGGAGTGGAGGATGAGGACCCCAAGAGGCCGTTGCCTCGGAAACAGAGTGACTCCTCCACATATGACTGT
GAGGCGATCACCCAGCATCATGCCTTCCTCTCAAGCATCCACTCCAGTGTGCTGAAGGATGAGGCTGAGG
CTCCTGCAGCTGGCACCCCCCAGCTCTCTGAGGTCAGCCTGGGTCGCTTTGACTTTGATGTGTCTGACTT
CTTCCTCTTCGGCTCACCACTGGGCCTGGTCCTGGCCATGCGGAGGACAGTGCTGCCTGGGATAGATGGT
TTCCAGATGCGTCCTGCCTGTAGCCAGGTCTACAGCTTCTTCCACTGTGCAGATCCCTCTGCTTCACGGC
TTGAGCCACTGCTGGAGCCCAAGTTCCACCTGGTGCCTCCTGTCAGCGTGCCTCGCTACCAGAGGTTCCC
CCTGGGCGATGGACAGTCTCTCCTGCTCGCTGATGCCCTGCACACCCACAGCCCCCTATTCCTGGAGGGA
AGCTCCCGGGGAAGCCCCCCGCTTCTGGATGCCCCTGCCTCGCCCCCTCAGGCCCCAAGATTCCAGCGCA
CAGAACGGAGGCTAAGTAAAGGCAGCTCCCATAGCGACAGTTCAGAGTCCTCGGACAGCCTGGCGCCTAT
GGGTGCCTCCCGCATCACAGCCAAGTGGTGGGGAACCAAGAGGATTGACTATGCCCTGTACTGCCCAGAT
GTCCTCACCGCCTTCCCCACAGTGGCTCTGCCTCACCTCTTCCATGCCAGCTACTGGGAGTCTACCGATG
TGGTCGCCTTCATCTTGAGACAGGTGATGCGTTACGAGAGTGCAAGTGTGAAGGAGAGCACTGGTCTGGA
CCCCACAGCTCTGAGCCCTGCTAACCCCCGTGAGAAGTGGCTGCGTAAGCGAACCCAGGTCAAGTTGCGG
AACGTTACTGCGAACCACCGTGCCAATGATGTAATTGCTGCTGAGGATGGCCCCCAGGTCTTGGTGGGGC
GCTTCATGTATGGCCCTCTTGACATGGTAGCTCTGACTGGAGAGAAGGTGGACATCCTGGTGATGACAGA
GCCGTCGTCAGGCCGCTGGGTCCACCTGGACACAGAAATCACCAACAACAGTGGCAGAATCACATACAAT
GTGCCCCGGCCCCGACGTCTGGGGGTTGGTGTCTACCCTGTAAAGATGGTGGTCAGGGGTGACCAGACCT
GTGCGATGAGCTACCTCACGGTGCTGCCCCGAGGCATGGAGTGTGTTGTGTTCAGCATTGACGGGTCCTT
TGCCGCCAGTGTGTCTATCATGGGAAGTGACCCCAAGGTCCGGCCAGGTGCAGTGGATGTGGTTCGGCAC
TGGCAGGACCTGGGCTACATGATCCTTTACATCACGGGGCGGCCGGACATGCAGAAGCAGCGGGTGGTGT
CGTGGCTGTCCCAGCACAACTTCCCACAGGGCATGATCTTTTTCTCGGACGGACTGGTGCACGACCCACT
GAGGCAGAAGGCCATCTTCCTTCGCAACCTCATGCAGGAGTGTTTCATCAAAATCACTGCTGCCTATGGC
TCCACGAAAGATATCTCTGTCTACAGTGTGCTGGGCCTCCCTGCCTCCCAGATCTTCATTGTAGGCCGGT
CCACCAAGAAGTACCAAACCCAGTGCCAGTTCCTGAGCGAAGGCTACGCAGCACACCTGGCGGCACTAGA
AGCCAGCCACCGCTCTCGCCCCAAGAAGAACAACTCGCGCATGATCCTGCGCAAGGGCAGCTTTGGGCTG
CACGCGCAGCCAGAATTTCTGCGCAAGCGCAACCACCTGCGCAGGACCATGTCGGTGCAGCAGCCGGACC
CACCGGCCGCCAACCCCAAGCCCGAGCGGGCGCAGAGCCAGCCCGAGTCCGACAAGGACCACGAGCGGCC
GCTGCCCGCTCTCAGCTGGGCGCGTGGGCCCCCTAAGTTCGAGTCGGTGCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218936 representing NM_001024927
Red=Cloning site Green=Tags(s)

MAKAGSAGGPSPGGGAPWHLRNVLSDSVESSDDEFFDAREEVAEGKNAILIGMSQWSSNDLVEQIETIGK
LDERQGDGATACTSSILQEKQRELYRVSLRRQRFPAQGSIEIHEDGEEGCSQRSCKTHVLLLVLHGGNVL
DTGSGDPSCKAADIHTFSSVLEKVMRAHFPAALGHILIKFVPCPAICSEAFSLVSNLNPYSHDEGCLGTS
QDHVPLAALPLLAISSPQYQDAVATVIERANHIYGEFLKSSDGIGFNGQVCLIGDCVGGLLAFDAICYSA
GPSGDSPGSSSRKGSISSTQDTPVVVEEDCSLASSKRLSKSNVDVSSGVEDEDPKRPLPRKQSDSSTYDC
EAITQHHAFLSSIHSSVLKDEAEAPAAGTPQLSEVSLGRFDFDVSDFFLFGSPLGLVLAMRRTVLPGIDG
FQMRPACSQVYSFFHCADPSASRLEPLLEPKFHLVPPVSVPRYQRFPLGDGQSLLLADALHTHSPLFLEG
SSRGSPPLLDAPASPPQAPRFQRTERRLSKGSSHSDSSESSDSLAPMGASRITAKWWGTKRIDYALYCPD
VLTAFPTVALPHLFHASYWESTDVVAFILRQVMRYESASVKESTGLDPTALSPANPREKWLRKRTQVKLR
NVTANHRANDVIAAEDGPQVLVGRFMYGPLDMVALTGEKVDILVMTEPSSGRWVHLDTEITNNSGRITYN
VPRPRRLGVGVYPVKMVVRGDQTCAMSYLTVLPRGMECVVFSIDGSFAASVSIMGSDPKVRPGAVDVVRH
WQDLGYMILYITGRPDMQKQRVVSWLSQHNFPQGMIFFSDGLVHDPLRQKAIFLRNLMQECFIKITAAYG
STKDISVYSVLGLPASQIFIVGRSTKKYQTQCQFLSEGYAAHLAALEASHRSRPKKNNSRMILRKGSFGL
HAQPEFLRKRNHLRRTMSVQQPDPPAANPKPERAQSQPESDKDHERPLPALSWARGPPKFESVP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_001024927
ORF Size 2922 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001024927.2, NP_001020098.1
RefSeq Size 6682 bp
RefSeq ORF 2925 bp
Locus ID 327958
UniProt ID Q3UHE1
Cytogenetics 11 B4
MW 106.5 kDa
Summary Catalyzes the transfer of phosphatidylinositol and phosphatidylcholine between membranes (in vitro). Binds calcium ions (By similarity).[UniProtKB/Swiss-Prot Function]
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SKU Description Size Price
MC222932 Pitpnm3 (untagged) - Mouse PITPNM family member 3 (Pitpnm3), transcript variant 1, (10ug) 10 ug
$982.00
MG218936 Pitpnm3 (tGFP-tagged) - Mouse PITPNM family member 3 (Pitpnm3) transcript variant 1, (10ug) 10 ug
$1,092.00
MR218936L3 Lenti ORF clone of Pitpnm3 (Myc-DDK-tagged) - Mouse PITPNM family member 3 (Pitpnm3), transcript variant 1 10 ug
$1,192.00
MR218936L4 Lenti ORF clone of Pitpnm3 (mGFP-tagged) - Mouse PITPNM family member 3 (Pitpnm3), transcript variant 1 10 ug
$1,192.00

Citations

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