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Pak7 (NM_172858) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218929
Pak7 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 7 (Pak7)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $658.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pak7
Synonyms 2900083L08Rik; 6430627N20; PAK-5; PAK-7; Pak5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218929 representing NM_172858
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTTGGGAAGAAAAAGAAAAAGATCGAAATATCTGGCCCATCCAACTTTGAACACAGGGTTCATACTG
GATTTGATCCACAAGAGCAGAAGTTTACTGGCCTTCCCCAGCAGTGGCACAGCCTGTTAGCAGACACAGC
CAACAGGCCCAAGCCCATGGTGGACCCATCATGCATCACACCCATACAGCTGGCTCCCATGAAGACAATC
GTCAGAGGAAATAAATCCTGCAAGGAAACCTCTATCAATGGTCTGCTAGAGGATTTTGACAACATCTCCG
TGACTCGCTCCAACTCTCTAAGGAAAGAAAGCCCACCCACCCCAGATCAGGGAGCAGCTAGCCGCATTCA
AGGCCACTCGGAAGAGAACGGCTTCATCACTTTCTCACAATATTCCAGTGAATCCGATACGACTGCGGAC
TACACAACTGAAAAGTACAGAGACAGGAGTCTCTATGGAGATGACCTGGATCTGTACTATAAAAGCAGCC
ATGCAGCCAAGCAAAATGGGCATGCCATGAAGATGAAACATGGAGACGCTTACTACCCTGAGATGAAGTC
TTTGAAAACCGACCTGGCCGGATTCCCTGTCGACTATCACACCCACTTGGACTCTCTGAGAAAATCAAGT
GAATATGGTGACCTTAGGTGGGATTATCAGAGAGCCTCTAGTAGCTCCCCTCTGGACTACTCATTCCAGC
TCACGCCTTCTAGAACTGCAGGGACCAGCAGGTGCTCCAAGGAGAGTCTGGCATACAGTGAAAGTGATTG
GGGACCCAGCCTGGATGACTATGACAGGAGGCCAAAATCATCATACCTGCATCAGACGAGCCCTCAGCCA
GCCATGCGCCAGAGATCCAAGTCCGGCTCAGGGCTTCAGGAACCCATGATGCCATTTGGAGCAAGTGCAT
TTAAAACTCATCCTCAAGGACACTCGTACAACTCCTACACCTACCCTCGATTGTCCGAGCCCACAATGTG
CATTCCAAAGGTGGATTACGATCGAGCACAGATGGTCTTCAGTCCTCCACTGTCAGGGTCCGACACTTAC
CCCAGAGGCCCCACCAAACTACCTCAAAGTCAAAGCAAAGCAGGCTACTCTTCAGGCAGCCACCAGTACC
CTTCTGGGTACCACAAAGCATCTCTATACCACCATCCATCCCTGCAAACCAGTTCTCAGTACATCTCCAC
CGCTTCTTACTTAAGCTCTCTCAGTATCTCCTCGAGCACCTACCCTCCACCTAGCTGGGGCTCCTCCTCA
GACCAGCAGCCCTCAAGGGTATCCCATGAACAATTCCGAGCTGCCCTGCAACTGGTGGTCAGCCCAGGAG
ACCCCAGGGAATATTTGGATAACTTTATTAAAATCGGAGAAGGGTCGACAGGCATCGTGTGCATTGCAAC
AGAGAAACACACAGGCAAGCAAGTGGCAGTGAAGAAAATGGACCTCCGAAAGCAGCAGAGACGGGAACTC
CTTTTTAATGAGGTCGTGATAATGCGTGATTACCACCATGACAACGTAGTTGACATGTACAACAGCTACC
TTGTTGGAGATGAGCTCTGGGTGGTCATGGAGTTTCTAGAAGGTGGTGCCTTGACAGACATTGTCACTCA
TACCAGAATGAATGAAGAGCAGATAGCTACTGTCTGCCTGTCAGTTCTGAAAGCTCTGTCCTACCTTCAT
AACCAAGGAGTGATTCACAGGGACATAAAGAGTGACTCCATTCTTCTGACAAGCGATGGCCGGATAAAGT
TATCTGACTTTGGTTTCTGTGCTCAAGTTTCCAAAGAGGTGCCAAAGAGGAAGTCACTGGTGGGTACCCC
ATACTGGATGGCACCTGAGGTGATTTCCAGGCTACCTTATGGGACAGAGGTGGACATCTGGTCCCTCGGG
ATAATGGTGATAGAGATGATTGATGGGGAGCCCCCCTATTTCAATGAGCCTCCTCTGCAGGCCATGAGGA
GGATCCGGGACAGTTTACCTCCAAGAGTGAAGGACCTACACAAGGTTTCTTCCATGCTCCGAGGATTCCT
AGATCTTATGTTGGTGAGGGAGCCCTCTCAAAGAGCCACAGCTCAAGAACTCCTTGGACATCCATTCTTA
AAATTGGCAGGTCCACCATCTTGCATTGTTCCTCTCATGAGACAATACAGACATCAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218929 representing NM_172858
Red=Cloning site Green=Tags(s)

MFGKKKKKIEISGPSNFEHRVHTGFDPQEQKFTGLPQQWHSLLADTANRPKPMVDPSCITPIQLAPMKTI
VRGNKSCKETSINGLLEDFDNISVTRSNSLRKESPPTPDQGAASRIQGHSEENGFITFSQYSSESDTTAD
YTTEKYRDRSLYGDDLDLYYKSSHAAKQNGHAMKMKHGDAYYPEMKSLKTDLAGFPVDYHTHLDSLRKSS
EYGDLRWDYQRASSSSPLDYSFQLTPSRTAGTSRCSKESLAYSESDWGPSLDDYDRRPKSSYLHQTSPQP
AMRQRSKSGSGLQEPMMPFGASAFKTHPQGHSYNSYTYPRLSEPTMCIPKVDYDRAQMVFSPPLSGSDTY
PRGPTKLPQSQSKAGYSSGSHQYPSGYHKASLYHHPSLQTSSQYISTASYLSSLSISSSTYPPPSWGSSS
DQQPSRVSHEQFRAALQLVVSPGDPREYLDNFIKIGEGSTGIVCIATEKHTGKQVAVKKMDLRKQQRREL
LFNEVVIMRDYHHDNVVDMYNSYLVGDELWVVMEFLEGGALTDIVTHTRMNEEQIATVCLSVLKALSYLH
NQGVIHRDIKSDSILLTSDGRIKLSDFGFCAQVSKEVPKRKSLVGTPYWMAPEVISRLPYGTEVDIWSLG
IMVIEMIDGEPPYFNEPPLQAMRRIRDSLPPRVKDLHKVSSMLRGFLDLMLVREPSQRATAQELLGHPFL
KLAGPPSCIVPLMRQYRHH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_172858
ORF Size 2157 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_172858.2, NP_766446.2
RefSeq Size 4773 bp
RefSeq ORF 2160 bp
Locus ID 241656
UniProt ID Q8C015
Cytogenetics 2 F3
MW 81.4 kDa
Summary Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell migration, proliferation or cell survival. Activation by various effectors including growth factor receptors or active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Phosphorylates the proto-oncogene RAF1 and stimulates its kinase activity. Promotes cell survival by phosphorylating the BCL2 antagonist of cell death BAD. Phosphorylates CTNND1, probably to regulate cytoskeletal organization and cell morphology. Keeps microtubules stable through MARK2 inhibition and destabilizes the F-actin network leading to the disappearance of stress fibers and focal adhesions (By similarity).UniProtKB/Swiss-Prot Function
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"NM_172858" in other vectors (4)
SKU Description Size Price
MC221051 Pak7 (untagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 7 (Pak7), (10ug) 10 ug
$725.00
MG218929 Pak7 (tGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 7 (Pak7), (10ug) 10 ug
$858.00
MR218929L3 Lenti ORF clone of Pak7 (Myc-DDK-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 7 (Pak7) 10 ug
$958.00
MR218929L4 Lenti ORF clone of Pak7 (mGFP-tagged) - Mouse p21 protein (Cdc42/Rac)-activated kinase 7 (Pak7) 10 ug
$958.00

Plasmid Map for MR218929

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218929
Circular map for MR218929

file-alt Citations

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