Plcd4 (NM_148937) Mouse Tagged ORF Clone
- TrueORF®
SKU
MR218890
Plcd4 (Myc-DDK-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 2
$589.00
$739.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Plcd4 |
| Synonyms | 4921507K24Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_148937 |
| ORF Size | 2421 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_148937.2, NP_683739.2 |
| RefSeq Size | 2981 bp |
| RefSeq ORF | 2424 bp |
| Locus ID | 18802 |
| UniProt ID | Q8K3R3 |
| Cytogenetics | 1 38.54 cM |
| MW | 92.7 kDa |
| Summary | Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca(2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca(2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_148937" in other vectors (3)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MG218890 | Plcd4 (tGFP-tagged) - Mouse phospholipase C delta 4 (Plcd4) transcript variant 2, (10ug) | 10 ug |
$939.00
|
|
| MR218890L3 | Lenti ORF clone of Plcd4 (Myc-DDK-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 2 | 10 ug |
$1,039.00
|
|
| MR218890L4 | Lenti ORF clone of Plcd4 (mGFP-tagged) - Mouse phospholipase C, delta 4 (Plcd4), transcript variant 2 | 10 ug |
$1,039.00
|
|
Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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