Fbxl16 (NM_001164225) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218862
Fbxl16 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 16 (Fbxl16)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Fbxl16
Synonyms BC042620; Scirr1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218862 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGAGCCCCGGTATCGATGGTGACCCCAAGCCTCCATGCTTGCCTCGAAACGGTCTGGTGAAGCTGC
CTGGCCAGCCCAACGGCCTAGGTGCAGCCAGCATCACCAAGGGCACACCTGCTGCCAAGAACCGTCCTTG
CCAGCCACCACCCCCACCGACCCTCCCACCTCCAAGCCTGGCTACACCACTATCCCGGGTTGCTCTGGCC
GGGGGGCCATGCCCCCCAGCCAGTGGACCAGCCTCGGGTCCAGTGTCTGGACCCCCAGTGGAGCGGCCAC
CACTGGCCACAGACGAGAAGATCCTTAATGGGCTTTTCTGGTATTTCTCAGCATGTGAGAAGTGCATACT
AGCTCAGGTGTGCAAGGCTTGGCGGCGTGTGCTCTACCAGCCCAAGTTCTGGGCGGGCCTCACGCCTGTG
CTGCATGCCAAGGAGCTGTACAACGTGCTGCCTGGAGGCGAGAAGGAGTTTGTGAACCTGCAGGGCTTTG
CTGCTAGAGGTTTTGAGGGCTTCTGCCTAGTTGGTGTCTCCGACTTAGACATCTGCGAGTTCATCGACAA
CTATTCTCTCTCTAAGAAGGGAGTCAAGGCCATGAGTCTCAAGCGCTCCACCATCACTGACGCTGGTCTA
GAGGTGATGCTGGAGCAGATGCAGGGAGTGGTGCGTCTGGAGCTGTCAGGCTGCAACGATTTCACCGAGG
CTGGCCTGTGGTCCAGCCTCAGTGCCCGTATCACCTCCCTGAGCGTCAGTGACTGCATCAATGTGGCAGA
TGATGCAATCGCTGCCATCTCACAACTTCTACCCAACCTGGCAGAGCTAAGCCTTCAGGCCTATCACGTG
ACTGACACGGCCCTGGCCTACTTCACAGCACGCCAGGGCCACAGCACCCACACGCTGCGCCTGCTTTCCT
GCTGGGAGATAACCAACCATGGGGTGGTCAATGTGGTGCACAGTCTGCCCAACCTCACCTCACTCAGCCT
CTCAGGCTGCTCTAAGGTCACTGATGATGGAGTGGAGCTTGTAGCTGAGAACCTGCGCAAATTGCGCAGC
CTTGACCTTTCCTGGTGCCCTCGGATCACCGATATGGCACTGGAGTATGTGGCCTGCGACTTGCACCGAC
TGGAGGAGCTCGTGCTGGACAGGTGTGTACGCATCACGGACACTGGTCTCAGCTATTTGTCCACCATGTC
GTCTCTCCGCAGCCTCTACCTGCGATGGTGCTGCCAGGTGCAGGACTTCGGGTTGAAGCACCTCTTAGCC
ATGAGAAATTTGCGACTCTTGTCTCTAGCAGGCTGCCCGCTCCTAACTACCACCGGGCTATCTGGCCTCG
TGCAACTGCAAGAGCTGGAGGAGCTGGAGCTGACCAACTGCCCTGGAGCCACACCCGAGCTCTTCAAGTA
CTTCTCGCAGCACCTGCCGCGCTGCCTCGTCATTGAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218862 protein sequence
Red=Cloning site Green=Tags(s)

MSSPGIDGDPKPPCLPRNGLVKLPGQPNGLGAASITKGTPAAKNRPCQPPPPPTLPPPSLATPLSRVALA
GGPCPPASGPASGPVSGPPVERPPLATDEKILNGLFWYFSACEKCILAQVCKAWRRVLYQPKFWAGLTPV
LHAKELYNVLPGGEKEFVNLQGFAARGFEGFCLVGVSDLDICEFIDNYSLSKKGVKAMSLKRSTITDAGL
EVMLEQMQGVVRLELSGCNDFTEAGLWSSLSARITSLSVSDCINVADDAIAAISQLLPNLAELSLQAYHV
TDTALAYFTARQGHSTHTLRLLSCWEITNHGVVNVVHSLPNLTSLSLSGCSKVTDDGVELVAENLRKLRS
LDLSWCPRITDMALEYVACDLHRLEELVLDRCVRITDTGLSYLSTMSSLRSLYLRWCCQVQDFGLKHLLA
MRNLRLLSLAGCPLLTTTGLSGLVQLQELEELELTNCPGATPELFKYFSQHLPRCLVIE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164225
ORF Size 1437 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164225.2
RefSeq Size 3499 bp
RefSeq ORF 1440 bp
Locus ID 214931
UniProt ID A2RT62
Cytogenetics 17 A3.3
MW 51.9 kDa
Summary Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001164225" in other vectors (4)
SKU Description Size Price
MC216709 Fbxl16 (untagged) - Mouse F-box and leucine-rich repeat protein 16 (Fbxl16), (10ug) 10 ug
$503.00
MG218862 Fbxl16 (tGFP-tagged) - Mouse F-box and leucine-rich repeat protein 16 (Fbxl16), (10ug) 10 ug
$657.00
MR218862L3 Lenti ORF clone of Fbxl16 (Myc-DDK-tagged) - Mouse F-box and leucine-rich repeat protein 16 (Fbxl16) 10 ug
$757.00
MR218862L4 Lenti ORF clone of Fbxl16 (mGFP-tagged) - Mouse F-box and leucine-rich repeat protein 16 (Fbxl16) 10 ug
$757.00

Plasmid Map for MR218862

Download sequence for

Cloning scheme for MR218862

Circular map for MR218862

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