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Dcp1b (NM_001033379) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218839
Dcp1b (Myc-DDK-tagged) - Mouse DCP1 decapping enzyme homolog b (S. cerevisiae) (Dcp1b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $538.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Dcp1b
Synonyms B930050E02Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218839 representing NM_001033379
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCCGCGGCGGCAGGTGGCCTGCCTGGGAAGGGACACGATATCAGCCTGGCAGCCCTGCGGCGCC
ACGACCCCTACATCAGTCGCATCGTGGACGTGGCCAGCCAGGTGGCTCTGTACACTTTCGGCCATCGGGC
CAACGAGTGGGAGAAGACTGGAGTGGAGGGAACCTTGTTTGTTTATACGAGGTCTGCCTCCCCAAAGCAC
GGATTCACCATTATGAATAGGCTCAGCATGGAAAACAGAACAGAACCCATTACTAAAGACCTGGACTTCC
AGCTCCAGAACCCTTTCCTTCTCTACAGAAACGGCACACTGTCCATTTATGGAATTTGGTTTTATGATAA
GGAAGAGTGCCAGAGAATTGCAAAACTAATGAAAAACCTCACTCAGAGCGAACAGTTGAAAGCCTGTCAT
GGAGCCGGATCCTCCCCCGTGACCTTGAGCTCAGGAGAGGGCCAAGAAGTAGATATCCTGCAGATGCTCA
CCAAGGCCAAGGATGAGTACACCAAGTGTAAGACCTGTTCCGAGCCAAAACAGATGACCAATTCCTCTGC
CATCTGTGACAACCCTAAACTTATCAAACCTGTCCCCGTGAGACCCAGCAGCAGCCAGAGGCTGCAAGGA
CCTGCGCCCAGCAAGACCTCGGACCCTGAGCCTCAGCACTTATCTTTAACAGCACTATTTGGGAAACAAG
ACAAAGCTCCCTGTCAGGAAACTGTAAAGCCCTCCCGGACCTTTGCCCACCACCACCACCATCACCACCA
GCAGCAGCAAGAGACGCGTCCAGTTCATCACGGGGTCGCATGCTCCCTGTCCTGTGAGGAGCCCAGGAAG
CTCTCACTCCCGGTGGAGAAGCAGCTCTGCCCGGCCATTCAGAAACTCATGCTCGGGAGCCCGGGGCTGC
ACCCCCTGCCTCAGCACCCTGAACAGTGGTCCTGCAAAAGTGGCAGCCCCAGTCCTGCCGGGGGAATTCT
CCCTGGTCCTGTCCAGCTGGGGTCCCCCTGGAATGGCAGGGTTGCACACTGCACACAGAGCACTTGCAGA
GGCCACAAGCTATTAGAGCAGCTTCAAGGTGCCCCTGGGGCAGTGCATAAGTATAACCCCTGTGCGCCTA
CCGGTCCAGCTGTGGCCACTCAGGTGGCTCCGGGCCAGAGTGTGGCCCAATCACAGCTGGTGTATTTCAG
TGGCCCCCTTCAACCTCCCGCCCCAGGACACCAAGCTCTCAGGAAGGAGCAGGGTGCACTGCCTGCACAA
GCGGTGTCCCTCTCTGGCAGCCAGGAGAGCAGCCCCACCGTGCTCCCTACGCAGGAGCTGCTAAGGAAGC
TCCAGGTAGTACACCAGGAGCAGCAGGCGGCCCCCCGGCCAGCCTTGGCAGCCAGGTTCCCTGTGTCAGC
CCAGGGATCAGGGACAGAAAAGCCCTTGGAAGCCTGGGTCAGCAAGACAGCCAGCATGGAGAAGCAGGCT
CCTCTTCTGCAGTCTACCTGTGCCCCACTGAGGGAGACTGATAATGGGCTCATGGCCCTAGGAGGCCAAG
AACTTCCTGCTGCCTCCTCCAGCCTCCTCCTGCCCCTGCAGAACTGGGAGTCCTCCACCGTGGCCAGCAG
GCCCCTCACCAGGCTGCAGCTCCAGGAGGCACTGCTGAACCTCATCCAGAATGATGACAACTTCTTAAGC
ATAATCTATGAAGCCTATCTCTTCAGCGTGACACAAGCAGCCATGAGAACCACG

AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218839 representing NM_001033379
Red=Cloning site Green=Tags(s)

MAAAAAGGLPGKGHDISLAALRRHDPYISRIVDVASQVALYTFGHRANEWEKTGVEGTLFVYTRSASPKH
GFTIMNRLSMENRTEPITKDLDFQLQNPFLLYRNGTLSIYGIWFYDKEECQRIAKLMKNLTQSEQLKACH
GAGSSPVTLSSGEGQEVDILQMLTKAKDEYTKCKTCSEPKQMTNSSAICDNPKLIKPVPVRPSSSQRLQG
PAPSKTSDPEPQHLSLTALFGKQDKAPCQETVKPSRTFAHHHHHHHQQQQETRPVHHGVACSLSCEEPRK
LSLPVEKQLCPAIQKLMLGSPGLHPLPQHPEQWSCKSGSPSPAGGILPGPVQLGSPWNGRVAHCTQSTCR
GHKLLEQLQGAPGAVHKYNPCAPTGPAVATQVAPGQSVAQSQLVYFSGPLQPPAPGHQALRKEQGALPAQ
AVSLSGSQESSPTVLPTQELLRKLQVVHQEQQAAPRPALAARFPVSAQGSGTEKPLEAWVSKTASMEKQA
PLLQSTCAPLRETDNGLMALGGQELPAASSSLLLPLQNWESSTVASRPLTRLQLQEALLNLIQNDDNFLS
IIYEAYLFSVTQAAMRTT

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033379
ORF Size 1734 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033379.3, NP_001028551.2
RefSeq Size 3311 bp
RefSeq ORF 1737 bp
Locus ID 319618
Cytogenetics 6 F1
MW 63.1 kDa
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Documents
Resources
"NM_001033379" in other vectors (4)
SKU Description Size Price
MC219240 Dcp1b (untagged) - Mouse DCP1 decapping enzyme homolog b (S. cerevisiae) (Dcp1b), (10ug) 10 ug
$593.00
MG218839 Dcp1b (tGFP-tagged) - Mouse DCP1 decapping enzyme homolog b (S. cerevisiae) (Dcp1b), (10ug) 10 ug
$738.00
MR218839L3 Lenti ORF clone of Dcp1b (Myc-DDK-tagged) - Mouse DCP1 decapping enzyme homolog b (S. cerevisiae) (Dcp1b) 10 ug
$838.00
MR218839L4 Lenti ORF clone of Dcp1b (mGFP-tagged) - Mouse DCP1 decapping enzyme homolog b (S. cerevisiae) (Dcp1b) 10 ug
$838.00

Plasmid Map for MR218839

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218839
Circular map for MR218839

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