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Sp6 (NM_031183) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218829
Sp6 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 6 (Sp6)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Sp6
Synonyms 1110025J03Rik; AA591031; AI592962; Epfn; Klf14
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218829 representing NM_031183
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTAACCGCTGTCTGTGGCTCTCTGGGCAGCCAGCACACCGACGCGCCTCACGCTTCACCGCCGCGCC
TCGACCTGCAACCTCTCCAGACATACCAGGGTCACACGAGCCCGGAGGCTGGGGACTACCCCTCCCCGCT
GCAGCCTGGAGAGCTGCAGAGCCTCCCGCTGGGCCCGGAGGTAGACTTTTCACAGGGCTATGAGTTGCCA
GGGGCCTCCTCGCGGGTAACCTGCGAGGACCTGGAAAGTGACAGTCCCTTGGCACCGGGACCCTTTTCCA
AGCTCCTGCAGCCGGACATGTCACACCACTATGAATCGTGGTTCCGGCCGACTCACCCAGGCACAGAGGA
TGGCTCGTGGTGGGACCTTCATCCCGGCACCAGCTGGATGGACCTCCCCCATACTCAGGGAGCGCTGACC
TCACCTGGCCATCCGGGGGCGCTTCAGCCTGCTTTGGGAGGATACGTCGGAGACCATCAGCTCTGTGCTC
CGCCGCCCCACCCGCACCCGCACCACCTCCTCCCAGCCGCTGGTGGGCAGCACCTTCTGGGGCCACCCGA
CGGGGCTAAGGCCTTGGAAGCGGCCGCACAAGAGTCCCAAGGGCTGGATTCCAGTCTGGATGCAGCGTCC
CGACCCAAAGGCTCCCGGCGATCAGTTCCCCGCAGCTCAGGGCAGACCGTGTGTCGCTGCCCCAACTGCC
TGGAGGCGGAGCGACTCGGGGCTCCGTGCGGGCCCGATGGGGGCAAGAAGAAGCATTTGCACAACTGCCA
CATCCCAGGCTGCGGCAAGGCATACGCTAAGACGTCGCATCTGAAGGCGCACCTGCGGTGGCACAGCGGT
GACCGACCCTTCGTGTGCAACTGGCTTTTCTGCGGCAAGCGCTTCACGCGCTCCGACGAGCTGCAGCGCC
ACCTTCAGACCCACACCGGGACCAAGAAGTTCCCCTGTGCGGTCTGCAGCCGAGTCTTCATGCGCAGCGA
CCACCTGGCCAAACACATGAAAACCCACGAAGGCGCCAAAGAGGAGGCGGCGGCGGCGGCCCAAGGCGAG
GGCAAGGCCGGTGGCGTGGTGGAACCACCCGGGGGCAAAGGCAAACGTGAGGCCGAAGGCAGCTCGGCGT
CCTCCAAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218829 representing NM_031183
Red=Cloning site Green=Tags(s)

MLTAVCGSLGSQHTDAPHASPPRLDLQPLQTYQGHTSPEAGDYPSPLQPGELQSLPLGPEVDFSQGYELP
GASSRVTCEDLESDSPLAPGPFSKLLQPDMSHHYESWFRPTHPGTEDGSWWDLHPGTSWMDLPHTQGALT
SPGHPGALQPALGGYVGDHQLCAPPPHPHPHHLLPAAGGQHLLGPPDGAKALEAAAQESQGLDSSLDAAS
RPKGSRRSVPRSSGQTVCRCPNCLEAERLGAPCGPDGGKKKHLHNCHIPGCGKAYAKTSHLKAHLRWHSG
DRPFVCNWLFCGKRFTRSDELQRHLQTHTGTKKFPCAVCSRVFMRSDHLAKHMKTHEGAKEEAAAAAQGE
GKAGGVVEPPGGKGKREAEGSSASSN

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_031183
ORF Size 1128 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_031183.3
RefSeq Size 3375 bp
RefSeq ORF 1131 bp
Locus ID 83395
UniProt ID Q9ESX2
Cytogenetics 11 D
MW 40.5 kDa
Summary Promotes cell proliferation.UniProtKB/Swiss-Prot Function
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Documents
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"NM_031183" in other vectors (4)
SKU Description Size Price
MC211957 Sp6 (untagged) - Mouse trans-acting transcription factor 6 (Sp6), (10ug) 10 ug
$503.00
MG218829 Sp6 (tGFP-tagged) - Mouse trans-acting transcription factor 6 (Sp6), (10ug) 10 ug
$657.00
MR218829L3 Lenti ORF clone of Sp6 (Myc-DDK-tagged) - Mouse trans-acting transcription factor 6 (Sp6) 10 ug
$757.00
MR218829L4 Lenti ORF clone of Sp6 (mGFP-tagged) - Mouse trans-acting transcription factor 6 (Sp6) 10 ug
$757.00

Plasmid Map for MR218829

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218829
Circular map for MR218829

file-alt Citations

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