Serpinb1c (NM_173051) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218815
Serpinb1c (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 1c (Serpinb1c)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Serpinb1c
Synonyms EIC; ovalbumin
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218815 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGCAGCTGAGTTCAGCCAACAACCTCTTTGCTTTGGAGCTGTTCCACACCCTGAATGAGAGCAACC
CCACAGGAAATACCATCTTCTCTCCCGTCAGCATTTCTTCTGCCTTGGCCATGGTCTATCTGGGGGCCAG
AGGCAGCACTGCAGCCCAACTCTCTAAGACTCTGCATTTTGACTCTGCTGAGGACATCCATTCACAGTTT
CAAAGCCTGACTGCTGAAGTGAGCAAGCGTGGAGCATCTCACACTCTGAAACTTGCTAACAGACTGTATG
GAGAGAAAACCTACAACTTCCTTCCTGAGTACTTGGCTTCAATCCAGAAAACGTACAGTGCTGACTTGGC
CCTTGTGGATTTTCAGCATGCTTCTGAGGATGCAAGGAAGGAGATAAACCAGTGGGTCAAAGGTCAAACA
GAAGAGAAAATTCAGGAACTGTTTGCTGTGGGTGTGGTGGACAGTATGACTAAACTTGTACTAGTGAATG
CCACCTATTTCAAGGGAATGTGGCAGAAAAAATTCATGGCACGGGACACAACCGATGCTCCATTCCGACT
GAGTAAGAAAGTCACAAAAACAGTGAAGATGATGTATCTAAAGAATAATCTTCCATTTGGCTACATTCCA
GACCTGAAGTGCAAGGTGCTGGAGATGCCTTACCAGGGTGGAGAACTTAGCATGGTCATTCTGCTGCCTG
AAGACATTGAGGACGAGACCACAGGTCTTGAGGAGATTGAGAAACAATTAACTTTGGAAAAACTGCAAGA
ATGTGAGAACTTACAAAACATTGATGTCTGTGTCAAATTGCCCAAATTCAAGATGGAAGAGAGCTACATC
CTCAACTCTAACCTGGGCCAACTGGGAGTGCAGGATCTCTTTAGCAGCAGCAAAGCTGATCTCTCTGGCA
TGTCGGGATCCAGAGATCTTTTCATATCAAAAATTGTTCACAAGTCCTATGTGGAAGTGAATGAGGAGGG
AACAGAGACAGATGCTGCCATGCCAGGCACTGTTGTTGGATGCTGCCTGATGCCCATGGAATTCACGGTG
GACCATCCATTCCTTTTCTTCATTCGGCACAATCCCACGGCTCATGTGCTCTTCCTTGGCAGGGTTTGTT
CCCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218815 protein sequence
Red=Cloning site Green=Tags(s)

MGQLSSANNLFALELFHTLNESNPTGNTIFSPVSISSALAMVYLGARGSTAAQLSKTLHFDSAEDIHSQF
QSLTAEVSKRGASHTLKLANRLYGEKTYNFLPEYLASIQKTYSADLALVDFQHASEDARKEINQWVKGQT
EEKIQELFAVGVVDSMTKLVLVNATYFKGMWQKKFMARDTTDAPFRLSKKVTKTVKMMYLKNNLPFGYIP
DLKCKVLEMPYQGGELSMVILLPEDIEDETTGLEEIEKQLTLEKLQECENLQNIDVCVKLPKFKMEESYI
LNSNLGQLGVQDLFSSSKADLSGMSGSRDLFISKIVHKSYVEVNEEGTETDAAMPGTVVGCCLMPMEFTV
DHPFLFFIRHNPTAHVLFLGRVCSP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_173051
ORF Size 1125 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_173051.2, NP_766639.2
RefSeq Size 1599 bp
RefSeq ORF 1128 bp
Locus ID 380839
UniProt ID Q5SV42
Cytogenetics 13 13.76 cM
MW 41.9 kDa
Summary Regulates the activity of the neutrophil proteases. Forms only a stable complex with CTSG/Cathepsin G (By similarity). During inflammation, limits the activity of inflammatory caspases CASP1 and CASP4 by suppressing their caspase-recruitment domain (CARD) oligomerization and enzymatic activation (PubMed:30692621).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_173051" in other vectors (4)
SKU Description Size Price
MC214678 Serpinb1c (untagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 1c (Serpinb1c), (10ug) 10 ug
$503.00
MG218815 Serpinb1c (tGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor clade B member 1c (Serpinb1c), (10ug) 10 ug
$657.00
MR218815L3 Lenti ORF clone of Serpinb1c (Myc-DDK-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 1c (Serpinb1c) 10 ug
$757.00
MR218815L4 Lenti ORF clone of Serpinb1c (mGFP-tagged) - Mouse serine (or cysteine) peptidase inhibitor, clade B, member 1c (Serpinb1c) 10 ug
$757.00

Plasmid Map for MR218815

Download sequence for

Cloning scheme for MR218815

Circular map for MR218815

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