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Vps37d (NM_177574) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218668
Vps37d (Myc-DDK-tagged) - Mouse vacuolar protein sorting 37D (yeast) (Vps37d), transcript variant 2
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Vps37d or NM_177574"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Vps37d
Synonyms Wbscr24
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218668 representing NM_177574
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTACCGGGCCCGGGCGGCGCGGGCGGGGCCGGAGCCCGGCAGCCCGGGGCGCTTTGGGATCCTCAGCA
CCGGGCAGCTCCGGGATCTGCTTCAAGATGAGCCCAAGCTGGACCGGATCGTGCGGCTCAGCAGGAAGGT
CCAGATGGAGCAGCTGCTGCTGGGGGAACAGAGCCTGGAGGCCTTCCTGCCAGCCTTCCAGAGGGGCCGT
GCCCTGGCCCACCTGAGGCGGACACAGGCCGAGAAACTACAGGAGGTGCTGAGGCGTCGAGAGCGCTCTG
CCCAGCCGGCCCCCACAACTGCTGCTGCTGCTGCCGCCGCCGCCACCGCCATGGATCCCCCCAAACCCTT
CCCTGCTGCTGCCGTCCTGCCCACTGGGGCCGCCCGGGGACCACCTCCAGCAGTACCCCGGAGCCTGCCC
CCCTTGGACTCCCGCCCAGTGCCCCCAGTGAAGGGCTCTCCCGGGTGCCCTTTTGGCCCAGCCCCTTTGC
TGAGCCCTCGACCCTCTCAGCCTGAACCCCCTCATCGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218668 representing NM_177574
Red=Cloning site Green=Tags(s)

MYRARAARAGPEPGSPGRFGILSTGQLRDLLQDEPKLDRIVRLSRKVQMEQLLLGEQSLEAFLPAFQRGR
ALAHLRRTQAEKLQEVLRRRERSAQPAPTTAAAAAAAATAMDPPKPFPAAAVLPTGAARGPPPAVPRSLP
PLDSRPVPPVKGSPGCPFGPAPLLSPRPSQPEPPHR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177574
ORF Size 528 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177574.4, NP_808242.1
RefSeq Size 1306 bp
RefSeq ORF 531 bp
Locus ID 194309
UniProt ID Q810I0
Cytogenetics 5 G2
MW 18.8 kDa
Summary Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. May be involved in cell growth and differentiation (By similarity).UniProtKB/Swiss-Prot Function
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"NM_177574" in other vectors (4)
SKU Description Size Price
MC212458 Vps37d (untagged) - Mouse vacuolar protein sorting 37D (yeast) (Vps37d), transcript variant 2, (10ug) 10 ug
$330.00
MG218668 Vps37d (tGFP-tagged) - Mouse vacuolar protein sorting 37D (yeast) (Vps37d), (10ug) 10 ug
$500.00
MR218668L3 Lenti ORF clone of Vps37d (Myc-DDK-tagged) - Mouse vacuolar protein sorting 37D (yeast) (Vps37d), transcript variant 2 10 ug
$600.00
MR218668L4 Lenti ORF clone of Vps37d (mGFP-tagged) - Mouse vacuolar protein sorting 37D (yeast) (Vps37d), transcript variant 2 10 ug
$600.00

Plasmid Map for MR218668

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218668
Circular map for MR218668

file-alt Citations

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