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Psmf1 (NM_212446) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218495
Psmf1 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Psmf1 or NM_212446"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Psmf1
Synonyms AW048666; BC012260; PI31
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218495 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGGTCTCGAGGTTCTCTTCGCGTCGGCCGCTCCATCCATGAGCTGCCCGCAGGACGCACTCGTCT
GCTTCCTGCACTGGGAAGTGGTGACAAACGGCTACTATGCCTTGGGCACGGGCGACCAGCCAGGTCCCAG
TGATAAGAAATCAGAACTACTGCCAGCTAAGTGGAACAGCAATAAAGAACTGTATGCCCTCCGGTATGAG
TCTAAGGATGGAGCCAGAAAACTCCTTCTGAAAGCTGTCTCTGTGGAGAACGGCATGATCATCAACGTGC
TGGAACTTGGCACACAGCAGGTGGCAGACTTGACCCTGAACTTAGATGATTATATTGATGCAGAAGACCT
GAGTGACTTCCACAGGACCTATAAGAACAGTGAGGAGCTTCGGTCTCAGATCAGATCTGGAATTATCACA
CCTATCCATGAGCAGTGGGAGAAGGCTCGTGCAAACAGTCCTCCGAGGGAGTTCCCACCTGCCACTGCCA
GAGAGGTGGACCCACTCCAGATTTCCTCACACCGTCCTCATACTAGCAGGCAGCCTGCTTGGCGTGATCC
CCTGAGCCCATTTGCTGTCGGGGGAGATGACCTAGACCCTTTTGGGTGTCAGAGAGGTGGCATGATTGTG
GATCCCCTGAGATCCGGCTTCCCAAGAGTACTTATTGACCCATCCTCAGGCCTCCCAAACCGGCTTCCTC
CTGGTGCTGTGCCCCCAGGAGCTCGCTTTGACCCCTTTGGACCCATTGGGACCAGCCCATCTGGACCTAA
CCCTGACCATCTTCCCCCACCGGGCTACGATGACATGTACCTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218495 protein sequence
Red=Cloning site Green=Tags(s)

MAGLEVLFASAAPSMSCPQDALVCFLHWEVVTNGYYALGTGDQPGPSDKKSELLPAKWNSNKELYALRYE
SKDGARKLLLKAVSVENGMIINVLELGTQQVADLTLNLDDYIDAEDLSDFHRTYKNSEELRSQIRSGIIT
PIHEQWEKARANSPPREFPPATAREVDPLQISSHRPHTSRQPAWRDPLSPFAVGGDDLDPFGCQRGGMIV
DPLRSGFPRVLIDPSSGLPNRLPPGAVPPGARFDPFGPIGTSPSGPNPDHLPPPGYDDMYL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_212446
ORF Size 813 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_212446.2
RefSeq Size 3542 bp
RefSeq ORF 816 bp
Locus ID 228769
UniProt ID Q8BHL8
Cytogenetics 2 G3
MW 29.7 kDa
Summary Plays an important role in control of proteasome function. Inhibits the hydrolysis of protein and peptide substrates by the 20S proteasome. Also inhibits the activation of the proteasome by the proteasome regulatory proteins PA700 and PA28 (By similarity).UniProtKB/Swiss-Prot Function
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"NM_212446" in other vectors (4)
SKU Description Size Price
MC212831 Psmf1 (untagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1), (10ug) 10 ug
$300.00
MG218495 Psmf1 (tGFP-tagged) - Mouse proteasome (prosome macropain) inhibitor subunit 1 (Psmf1), (10ug) 10 ug
$500.00
MR218495L3 Lenti ORF clone of Psmf1 (Myc-DDK-tagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1) 10 ug
$600.00
MR218495L4 Lenti ORF clone of Psmf1 (mGFP-tagged) - Mouse proteasome (prosome, macropain) inhibitor subunit 1 (Psmf1) 10 ug
$600.00

Plasmid Map for MR218495

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218495
Circular map for MR218495

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