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Shc4 (NM_199022) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218423
Shc4 (Myc-DDK-tagged) - Mouse SHC (Src homology 2 domain containing) family, member 4 (Shc4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $583.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Shc4
Synonyms 6230417E10Rik; 9930029B02Rik; Gm685; RaLP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218423 representing NM_199022
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGAGAACGCAGCCAGGACAGCCAGGCAGGACTCACGCTGTATGTAGGACTCTTTGGGCACCTGGGGA
TGCTTCACAGGACCAAGTACAGCCGCTTTCGGAACGAGTCCATCACGTCTTTGGACGAAGGGAGCCCCGG
AGGCTCCGTTGGAAACAAGGGCTCGTCGCCGCCTCCCTACCCAGCCCTGGCCCCTCACCTGCCGACTGAG
GATGCCACTGTGTCCAGCCAGGAGAGCCCCACGGCGCTGTGCACCTTGATTCCCCGTATGGCAAGCATGA
AGCTGGCCAACCCCATCACTTTTCTGGGTCTGAAAACCTTCTGCTTGGGTACGAAACAGGTGAGCCGGTT
GAAGCTCCAAGAGAACCAGGATCAGACTCCAAGCCGTCCAGCGTCCCCAGAATCCAATCTAAACAGGACT
GGACCTGCACCTGCACCGGATCCGGACCAGGTGGGACGCAGGCCCACCTCTTTGCGTCCTGATACTTGCC
CACTGCCTGGCCCTGGAGAACCAAGCCCCAGGAGCAAGCAGGACGGGCCCCCTCTGCAGCACCTGTTGGG
GAACGGTCTGAACTACTGTGTGCGGTACATGGGCTGCATTGAAGTGCTGCAATCAATGAGGTCACTGGAT
TTTGGAATGAGAACCCAAGTTACAAGGGAAGCAATAAGTCGCCTGTGTGAAGCTGTCCCCGGGGCCCATG
GAGCCATTAAAAAACGAAAGGCTCCCGTTAAGTTCCTGACAACGGTTCTTGGCAAAAGCAATCTTCAGTT
TTCAGGAATGAATATAAAACTGACTGTTTCAACAAGCAGCCTCACACTGATGAACCTTGACAACCAACAG
ATTATTGCAAATCACCAAATGCAATCGATTTCATTTGCCTCTGGAGGGGACCCTGATACCACAGACTATG
TTGCCTATGTAGCTAAAGATCCAGTCAACCAACGAGCGTGCCACATACTGGAGTGTCGCAGTGGAATGGC
CCAAGACGTCATAAGCACCATAGGGCAGGCCTTTGAACTCCGGTTCAAACAGTACTTGAAAAATCCTTCC
TTGAACACTTGGGAAAGGGAGGAGGTACTTGTTGATGGTGCCCCTGAAGACAGAGACCATGACTATTACA
ACTCCATCCCGGGGAAGCAGCCACCTGAAGGCGGCATTTCAGATGTGCGCATCCAGGCTCAAGCTACAGA
CCAAATGGCATACTGCCCTATCCGGTGTGAAAAGTTGTGCTATTTGCCTGGGAACTCCACGTGCAGCGGT
GTGTATAAGAACTGTATGGGACGGAGCAGGCCAATAGGTATCCCGCACGAGAGAGCGGGGCAGGGTGACA
CACCATCGCTGAGGCACTTCTGGAGAGTGGATCTCTTTGACGACCCCTGCTATGTCAATACACAGGCTCT
TCAGAGTATGCACAGCTATGCTGGAAATCAAAGCTCAGCTCTGCCACAGGGGAGCCCCTGGCACCTCGGA
AAGGCACCAGAAACTGTGCAGCCTGGAGCCACGGCCAAGCCTGGGAGCGCACTCGCTTTGCCACACATTA
GGCAGCAGCTGTGGGACGAGGAGTGCTTCCATGGCAAGCTGAGCAGAGGGGCAGCGGAGAAACTTTTGGT
GAAGGATGGGGACTTTTTGGTTCGAGAGAGTGTGACATCTCCCGGCCAGTTTGTACTGAGTGGACTGCAA
GGTGGCCAGGCCAAGCATCTTCTCTTGGTGGATCCAGAAGGCAAGGTGAGGACCAAGGACCATGTGTTTG
ATAATGTTGGCCACCTTATCAAATACCATATGGATAATAATTTGCCAATCATCTCTTCTGGAAGTGAAGT
ACGCCTTAAACAACCAATAAGAAAATATGATAACACAGGACTTTTGCCCCCCAAGAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218423 representing NM_199022
Red=Cloning site Green=Tags(s)

MRERSQDSQAGLTLYVGLFGHLGMLHRTKYSRFRNESITSLDEGSPGGSVGNKGSSPPPYPALAPHLPTE
DATVSSQESPTALCTLIPRMASMKLANPITFLGLKTFCLGTKQVSRLKLQENQDQTPSRPASPESNLNRT
GPAPAPDPDQVGRRPTSLRPDTCPLPGPGEPSPRSKQDGPPLQHLLGNGLNYCVRYMGCIEVLQSMRSLD
FGMRTQVTREAISRLCEAVPGAHGAIKKRKAPVKFLTTVLGKSNLQFSGMNIKLTVSTSSLTLMNLDNQQ
IIANHQMQSISFASGGDPDTTDYVAYVAKDPVNQRACHILECRSGMAQDVISTIGQAFELRFKQYLKNPS
LNTWEREEVLVDGAPEDRDHDYYNSIPGKQPPEGGISDVRIQAQATDQMAYCPIRCEKLCYLPGNSTCSG
VYKNCMGRSRPIGIPHERAGQGDTPSLRHFWRVDLFDDPCYVNTQALQSMHSYAGNQSSALPQGSPWHLG
KAPETVQPGATAKPGSALALPHIRQQLWDEECFHGKLSRGAAEKLLVKDGDFLVRESVTSPGQFVLSGLQ
GGQAKHLLLVDPEGKVRTKDHVFDNVGHLIKYHMDNNLPIISSGSEVRLKQPIRKYDNTGLLPPKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_199022
ORF Size 1878 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_199022.2, NP_950187.1
RefSeq Size 4820 bp
RefSeq ORF 1881 bp
Locus ID 271849
UniProt ID Q6S5L9
Cytogenetics 2 F1
MW 68.5 kDa
Summary Activates both Ras-dependent and Ras-independent migratory pathways in melanomas. Contributes to the early phases of agrin-induced tyrosine phosphorylation of CHRNB1.UniProtKB/Swiss-Prot Function
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"NM_199022" in other vectors (4)
SKU Description Size Price
MC219880 Shc4 (untagged) - Mouse SHC (Src homology 2 domain containing) family, member 4 (Shc4), (10ug) 10 ug
$642.00
MG218423 Shc4 (tGFP-tagged) - Mouse SHC (Src homology 2 domain containing) family member 4 (Shc4), (10ug) 10 ug
$783.00
MR218423L3 Lenti ORF clone of Shc4 (Myc-DDK-tagged) - Mouse SHC (Src homology 2 domain containing) family, member 4 (Shc4) 10 ug
$883.00
MR218423L4 Lenti ORF clone of Shc4 (mGFP-tagged) - Mouse SHC (Src homology 2 domain containing) family, member 4 (Shc4) 10 ug
$883.00

Plasmid Map for MR218423

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218423
Circular map for MR218423

file-alt Citations

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