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Samd4 (NM_001037221) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218353
Samd4 (Myc-DDK-tagged) - Mouse sterile alpha motif domain containing 4 (Samd4), transcript variant 1
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $651.00
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Other products for "Samd4 or NM_001037221"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Samd4
Synonyms 1700024G08Rik; 1700111L17Rik; 4933436G17Rik; Samd4a; Smaug; Smaug1; sunk
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218353 representing NM_001037221
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGATGTTTCGCGACCAGGTCGGGGTGCTGGCGGGCTGGTTTAAGGGCTGGAACGAGTGCGAGCAGACTG
TTGCACTGCTGTCGCTGCTCAAGCGCGTAAGCCAGACCCAGGCCCGCTTCCTTCAGCTCTGCCTGGAGCA
CTCGCTGGCCGACTGCGCCGAGCTGCACGTCCTGGAAGGAGAGGCCAACAGCCCGGGAATCATTAACCAA
TGGCAACAGGAATCCAAGGATAAAGTGATTTCCCTTCTGCTAACTCACCTGCCTTTGCTGAAGCCAGGAA
ACCTCGACGCGAAAGCAGAGTATATGAAACTGCTGCCCAAGATCCTGGCACACTCTATCGAACACAACCA
GCACATTGAGGAGAGCAGGCAGCTGCTGTCCTATGCTTTGATCCACCCAGCCACTTCCTTGGAAGACCGC
AGCGCACTAGCCATGTGGCTGAATCACTTGGAGGACCGCACATCCACCAGCTTTGGTAGCCAGAACCGAG
GCCGCTCGGACTCTGTGGATTATGGACAGACGCATTACTATCACCAAAGACAGAACTCTGACGATAAGCT
CAATGGCTGGCAGAACTCTCGGGATTCTGGGATTTGCATCAGTGCCTCCAACTGGCAGGACAAGAGCCTG
GGCTGTGAGAATGGCCATGTGCCCCTCTACTCTTCCTCGTCTGTCCCTGCCACAATCAACACGATTGGAA
CCGGCGCAAGCACGATTCTCTCAGGCCAGGCACACCACAGCCCTTTGAAACGATCTGTGTCCCTTACCCC
ACCCATGAATGTGCCAAACCAGCCTCTAGGACATGGATGGATGTCTCATGAGGACTTACGAGCTAGAGGA
CCTCAGTGCCTCCCATCCGATCATGCCCCCCTGTCTCCACAAAGCAGTGTAGCCTCTTCAGGAAGTGGTG
GGAGTGAACACTTAGAAGATCAGACCACTGCTCGTAACACATTCCAAGAAGAAGGTAGTGGTATGAAAGA
TGTTCCAGCTTGGTTAAAAAGCCTCCGCCTGCACAAGTACGCTGCGCTCTTCTCGCAGATGACTTATGAG
GAAATGATGGCCCTCACTGAGTGTCAACTGGAGGCTCAGAATGTTACCAAAGGCGCAAGACACAAAATTG
TAATCAGTATTCAGAAGCTCAAAGAAAGACAGAACCTCCTGAAGTCTTTGGAAAGGGACATCATCGAAGG
GGGCAGCCTGCGCACCCCCCTCCAGGAGCTACATCAGATGATCCTGACACCCATCAAGGCCTACAGCTCT
CCCAGTACCACCCCTGAGGTGCGCTGCCGGGAGCCCTCACTGATGGAGAGCCCAAGCCCTGACTGCAAAG
ACAGCGCTGCAGCAGTCACCAGCGCCACAGCCAGTGCCTCAGCCGGTGCCAGCGGTGGACTGCAACCACC
CCAGCTGAGCAGCTGCGACGGAGAGCTGGCTGTCGCCCCTCTGCCGGAGGGGGACCTCCCGGGGCAGTTC
ACCCGAGTCATGGGGAAAGTGTGCACACAGCTCTTGGTCTCCAGACCCGATGAAGAAAATATAAGCTCCT
ATCTACAGCTCTTAGACAAGTGTCTTGTTCATGAGGCATTTACAGAGACACAGAAAAAAAGATTGTTGTC
ATGGAAACAGCAGGTGCAGAAGCTCTTTCGGTCTTTCCCTCGGAAAACCCTTCTAGACATATCAGGATAT
CGACAGCAAAGAAATCGTGGCTTTGGGCAGTCCAACTCCCTCCCAACAGCCAGCTCTGTGGGCAGCGGCA
TGGGCAGACGGAACCCACGACAGTACCAGATCGCATCTCGGAATGTCCCTTCCGCCCGCCTTGGTCTCCT
GGGCACCAGTGGATTTGTCAGCTCCAACCAGCGCCACACCGCTGCCAACCCCACCATCATGAAACAAGGA
AGACAGAACCTCTGGTTTGCCAACCCTGGGGGCAGCAACAGCATGCCCAGCCGCACCCACAGCTCCGTCC
AGAAGACCCGCTCGCTGCCCGTGCATACTTCCCCACAAAACATGCTGATGTTCCAGCAACCAGAATTTCA
GCTTCCTGTGACTGAACCTGACATCAACAATAGGCTGGAGTCCTTGTGCCTCAGTATGACGGAGCATGCC
CTGGGAGACGGGGTTGACCGGACCTCCACAATC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218353 representing NM_001037221
Red=Cloning site Green=Tags(s)

MMFRDQVGVLAGWFKGWNECEQTVALLSLLKRVSQTQARFLQLCLEHSLADCAELHVLEGEANSPGIINQ
WQQESKDKVISLLLTHLPLLKPGNLDAKAEYMKLLPKILAHSIEHNQHIEESRQLLSYALIHPATSLEDR
SALAMWLNHLEDRTSTSFGSQNRGRSDSVDYGQTHYYHQRQNSDDKLNGWQNSRDSGICISASNWQDKSL
GCENGHVPLYSSSSVPATINTIGTGASTILSGQAHHSPLKRSVSLTPPMNVPNQPLGHGWMSHEDLRARG
PQCLPSDHAPLSPQSSVASSGSGGSEHLEDQTTARNTFQEEGSGMKDVPAWLKSLRLHKYAALFSQMTYE
EMMALTECQLEAQNVTKGARHKIVISIQKLKERQNLLKSLERDIIEGGSLRTPLQELHQMILTPIKAYSS
PSTTPEVRCREPSLMESPSPDCKDSAAAVTSATASASAGASGGLQPPQLSSCDGELAVAPLPEGDLPGQF
TRVMGKVCTQLLVSRPDEENISSYLQLLDKCLVHEAFTETQKKRLLSWKQQVQKLFRSFPRKTLLDISGY
RQQRNRGFGQSNSLPTASSVGSGMGRRNPRQYQIASRNVPSARLGLLGTSGFVSSNQRHTAANPTIMKQG
RQNLWFANPGGSNSMPSRTHSSVQKTRSLPVHTSPQNMLMFQQPEFQLPVTEPDINNRLESLCLSMTEHA
LGDGVDRTSTI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001037221
ORF Size 2133 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001037221.2, NP_001032298.1
RefSeq Size 7120 bp
RefSeq ORF 2136 bp
Locus ID 74480
UniProt ID Q8CBY1
Cytogenetics 14 C1
MW 78.8 kDa
Summary Acts as a translational repressor of SRE-containing messengers.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001037221" in other vectors (4)
SKU Description Size Price
MC220840 Samd4 (untagged) - Mouse sterile alpha motif domain containing 4 (Samd4), transcript variant 1, (10ug) 10 ug
$717.00
MG218353 Samd4 (tGFP-tagged) - Mouse sterile alpha motif domain containing 4 (Samd4) transcript variant 1, (10ug) 10 ug
$851.00
MR218353L3 Lenti ORF clone of Samd4 (Myc-DDK-tagged) - Mouse sterile alpha motif domain containing 4 (Samd4), transcript variant 1 10 ug
$951.00
MR218353L4 Lenti ORF clone of Samd4 (mGFP-tagged) - Mouse sterile alpha motif domain containing 4 (Samd4), transcript variant 1 10 ug
$951.00

Plasmid Map for MR218353

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218353
Circular map for MR218353

file-alt Citations

A Smaug2-Based Translational Repression Complex Determines the Balance between Precursor Maintenance versus Differentiation during Mammalian Neurogenesis
Amadei, Zander, Yang et al
J. Neurosci. (2015) 35 (47), 15666-81 DOI: 10.1523/JNEUROSCI.2172-15.2015
AI Summary: OriGene products contributed to this study by providing Flag-tagged expression constructs for mouse and human Smaug2 and Smaug1, as well as mouse Nanos1, Nanos2, Nanos3, and human Nanos1, which were used to investigate the roles of these proteins in neurogenesis.
Snippet: different mother. Plasmids and shRNAs. The Flag-tagged expression constructs for mouse and human Smaug2 and Smaug1 and mouse Nanos1, Nanos 2, and Nanos3 and human Nanos1 were obtained from OriGene. shRNA ... Cite SaveSaving
Showing 1-1 of 1 papers.
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