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1700024P04Rik (NM_027064) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218337
1700024P04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700024P04 gene (1700024P04Rik)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "1700024P04Rik or NM_027064"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol 1700024P04Rik
Synonyms H2bl1; Subh2bv
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218337 representing NM_027064
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAAGCCCACCTTCAAAAGACAATGCTACATCAAAAGACATCTAAGACCATTGTATAGAAAGCACT
CACGTTGCTCTAGCATCAATTTGGGCCATGGAAATTACTCCCTTTACATAAACAGAGTCCTGAAGGAAGT
TGTCCCTAACAGGGGTATATCATCCTATTCTGTGGACATCATGAACATCCTCATCAATGACATCTTCGAA
CGCATTGCTACGGAAGCCTGCCAGCAGATGTTCCTCAGAAAGCGCTGTACCCTCACCCCAGGAGATATCC
AGCAGGCAGTGCATCTGCTGTTGCCGAAGAAGCTGGCTACGCTTGCTGTGACTTTTGGAAGTAAAGCCGT
CCACAGATTCATTCATTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218337 representing NM_027064
Red=Cloning site Green=Tags(s)

MAKPTFKRQCYIKRHLRPLYRKHSRCSSINLGHGNYSLYINRVLKEVVPNRGISSYSVDIMNILINDIFE
RIATEACQQMFLRKRCTLTPGDIQQAVHLLLPKKLATLAVTFGSKAVHRFIHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027064
ORF Size 369 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027064.1, NP_081340.1
RefSeq Size 476 bp
RefSeq ORF 372 bp
Locus ID 69382
UniProt ID Q9D9Z7
Cytogenetics 13 D1
MW 14.2 kDa
Summary May act as an acrosome-nuclear docking protein in sperm.UniProtKB/Swiss-Prot Function
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Documents
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"NM_027064" in other vectors (4)
SKU Description Size Price
MC210922 1700024P04Rik (untagged) - Mouse RIKEN cDNA 1700024P04 gene (1700024P04Rik), (10ug) 10 ug
$165.00
MG218337 1700024P04Rik (tGFP-tagged) - Mouse RIKEN cDNA 1700024P04 gene (1700024P04Rik), (10ug) 10 ug
$350.00
MR218337L3 Lenti ORF clone of 1700024P04Rik (Myc-DDK-tagged) - Mouse RIKEN cDNA 1700024P04 gene (1700024P04Rik) 10 ug
$450.00
MR218337L4 Lenti ORF clone of 1700024P04Rik (mGFP-tagged) - Mouse RIKEN cDNA 1700024P04 gene (1700024P04Rik) 10 ug
$450.00

Plasmid Map for MR218337

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218337
Circular map for MR218337

file-alt Citations

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