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S100a13 (NM_009113) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218324
S100a13 (Myc-DDK-tagged) - Mouse S100 calcium binding protein A13 (S100a13)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $289.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "S100a13 or NM_009113"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol S100a13
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218324 representing NM_009113
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTAGTGACAGCAGGGGCGGTCCCCTCAGCGGAGGCGGAAACTTGACACTTCAGACTTGGGAGTGGC
TCTCGCCTTGCCTGGTGCTTATAAACTTCAGCCTCAGGGCTCCAGATCGGTTAGCTGCATCCCACCTTCT
TTCCCGGTCAATCTGTGTGGACATCAGCTCCTGCTTTGGTATACTGATGGCAGCAGAGACTCTGACTGAA
CTGGAGGCGGCCATTGAGACTGTGGTCTCTACTTTCTTCACCTTTGCAGGGCGGGAAGGACGCAAAGGCA
GCCTGAACATCAATGAATTTAAGGAACTGGCCACTCAGCAACTGCCTCATTTGCTCAAGGACGTGGGCTC
TCTAGATGAAAAGATGAAGACCTTGGATGTGAATCAGGACTCAGAGCTGAGGTTCAGTGAATACTGGAGA
CTGATTGGAGAGCTGGCAAAGGAAGTCAGGAAGGAGAAGGCCCTGGGGATCCGGAAGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218324 representing NM_009113
Red=Cloning site Green=Tags(s)

MASDSRGGPLSGGGNLTLQTWEWLSPCLVLINFSLRAPDRLAASHLLSRSICVDISSCFGILMAAETLTE
LEAAIETVVSTFFTFAGREGRKGSLNINEFKELATQQLPHLLKDVGSLDEKMKTLDVNQDSELRFSEYWR
LIGELAKEVRKEKALGIRKK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009113
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009113.4, NP_033139.2
RefSeq Size 732 bp
RefSeq ORF 483 bp
Locus ID 20196
UniProt ID P97352
Cytogenetics 3 39.24 cM
MW 18.2 kDa
Summary Plays a role in the export of proteins that lack a signal peptide and are secreted by an alternative pathway. Binds two calcium ions per subunit. Binds one copper ion. Binding of one copper ion does not interfere with calcium binding. Required for the copper-dependent stress-induced export of IL1A and FGF1. The calcium-free protein binds to lipid vesicles containing phosphatidylserine, but not to vesicles containing phosphatidylcholine.UniProtKB/Swiss-Prot Function
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"NM_009113" in other vectors (3)
SKU Description Size Price
MG218324 S100a13 (tGFP-tagged) - Mouse S100 calcium binding protein A13 (S100a13), (10ug) 10 ug
$350.00
MR218324L3 Lenti ORF clone of S100a13 (Myc-DDK-tagged) - Mouse S100 calcium binding protein A13 (S100a13) 10 ug
$450.00
MR218324L4 Lenti ORF clone of S100a13 (mGFP-tagged) - Mouse S100 calcium binding protein A13 (S100a13) 10 ug
$450.00

Plasmid Map for MR218324

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218324
Circular map for MR218324

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