Olfr1019 (NM_147015) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218306
Olfr1019 (Myc-DDK-tagged) - Mouse olfactory receptor 1019 (Olfr1019)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Olfr1019
Synonyms MOR180-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218306 representing NM_147015
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATAAAGAAAATCACTCAGTTGTGACTGAATTTGTCTTTATGGGCATAACTCAAGACCCTCAGCTGC
AGATCATCTTTTTTGTGGTCTTCCTCCTTGTCTACCTGGTCAATGTGATAGGGAATGTTGGTATGATCAT
CCTGATCATAACAGACAGTCAGCTTCACACCCCCATGTACTTTTTCCTCTGCAACCTCTCCTTTGTTGAC
CTGGGCTACTCATCAGCTATTGCTCCCAGGATGCTGGCTGATTTTCTAACAAAGCACAAAGTTATCTCTT
TTTCCAGCTGTGCCACCCAGTTTGCTTTCTTTGTAGGCTTTGTGGATGCTGAGTGCTATGTCCTGGCTGC
AATGGCCTATGACCGCTTTGTGGCCATCTGCAGACCTCTTCACTATAGCACTCTCATGTCCAAGAAAGTC
TGCTTGGTCCTAATGCTGGGCTCTTACTTCGCTGGCCTAGTGAGTTTAGTGGCGCATACTTCTCTCACTT
TCAGCCTCAGTTACTGTGGTTCCAATATCATCAACCATTTCTTCTGTGAAATCCCACCACTCTTAGCTCT
CTCTTGCTCAGATACCTACATTAGTGAGATACTGCTGTTTAGTTTGTGTGGCTTCATTGAGTTCAGCACC
ATCCTTATCATCTTCATCTCCTATGCCTTCATTCTCATTGCCATCATCAGAATTCGATCAGCTGAAGGCC
GGCTAAAGGCTTTTTCCACCTGCGGGTCTCATCTTACTGGTGTTACCCTTTTTTATGGTACAGTCATGTT
CATGTATCTGAGGCCAACATCCAGTTACTCCCTGGACCAAGACAAGTGGGCCTCTGTGTTCTACACAATT
ATCATTCCTATGCTGAACCCCTTGATCTACAGTTTGCGGAACAAGGATGTGAAAGCCGCTTTCAAGAAAC
TGATTGGAAAGAAGCCACAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218306 representing NM_147015
Red=Cloning site Green=Tags(s)

MDKENHSVVTEFVFMGITQDPQLQIIFFVVFLLVYLVNVIGNVGMIILIITDSQLHTPMYFFLCNLSFVD
LGYSSAIAPRMLADFLTKHKVISFSSCATQFAFFVGFVDAECYVLAAMAYDRFVAICRPLHYSTLMSKKV
CLVLMLGSYFAGLVSLVAHTSLTFSLSYCGSNIINHFFCEIPPLLALSCSDTYISEILLFSLCGFIEFST
ILIIFISYAFILIAIIRIRSAEGRLKAFSTCGSHLTGVTLFYGTVMFMYLRPTSSYSLDQDKWASVFYTI
IIPMLNPLIYSLRNKDVKAAFKKLIGKKPQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_147015
ORF Size 930 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_147015.1, NP_667226.1
RefSeq Size 933 bp
RefSeq ORF 933 bp
Locus ID 259017
UniProt ID Q8VGS3
Cytogenetics 2 D
MW 35.3 kDa
Summary Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_147015" in other vectors (4)
SKU Description Size Price
MC214138 Olfr1019 (untagged) - Mouse olfactory receptor 1019 (Olfr1019), (10ug) 10 ug
$330.00
MG218306 Olfr1019 (tGFP-tagged) - Mouse olfactory receptor 1019 (Olfr1019), (10ug) 10 ug
$500.00
MR218306L3 Lenti ORF clone of Olfr1019 (Myc-DDK-tagged) - Mouse olfactory receptor 1019 (Olfr1019) 10 ug
$600.00
MR218306L4 Lenti ORF clone of Olfr1019 (mGFP-tagged) - Mouse olfactory receptor 1019 (Olfr1019) 10 ug
$600.00

Plasmid Map for MR218306

Download sequence for

Cloning scheme for MR218306

Circular map for MR218306

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