Eprs (NM_029735) Mouse Tagged ORF Clone
$1,940.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Eprs |
| Synonyms | 2410081F06Rik; 3010002K18Rik; C79379; Qprs |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_029735 |
| ORF Size | 4536 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_029735.2 |
| RefSeq Size | 4850 bp |
| RefSeq ORF | 4539 bp |
| Locus ID | 107508 |
| UniProt ID | Q8CGC7 |
| Cytogenetics | 1 H5 |
| MW | 170.5 kDa |
| Summary | Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA (By similarity). The phosphorylation of EPRS, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3' UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS function from protein synthesis to translation inhibition (PubMed:23071094). Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan (PubMed:28178239).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_029735" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC224631 | Eprs (untagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs), (10ug) | 10 ug |
$2,070.00
|
|
| MG218237 | Eprs (tGFP-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs), (10ug) | 10 ug |
$2,140.00
|
|
| MR218237L3 | Lenti ORF clone of Eprs (Myc-DDK-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs) | 10 ug |
$2,240.00
|
|
| MR218237L4 | Lenti ORF clone of Eprs (mGFP-tagged) - Mouse glutamyl-prolyl-tRNA synthetase (Eprs) | 10 ug |
$2,240.00
|
|
Citations
Hypomyelinating Leukodystrophy 15 (HLD15)-Associated Mutation of EPRS1 Leads to Its Polymeric Aggregation in Rab7-Positive Vesicle Structures, Inhibiting Oligodendroglial Cell Morphological Differentiation
Sawaguchi, Goto, Kato et al
Polymers (Basel) (2021) 13 (7) DOI: 10.3390/polym13071074
Sawaguchi, Goto, Kato et al
Polymers (Basel) (2021) 13 (7) DOI: 10.3390/polym13071074
Cited by 7
AI Summary: OriGene products, specifically the EPRS1 Myc-DDK-tagged expression plasmid (Cat# RC211070) and EPRS1 (R339X) Myc-DDK-tagged mutant plasmid (Cat# RC211070NM), were used in this study to express wild-type and mutant EPRS1 proteins in oligodendroglial cells, enabling the investigation of their effects on protein localization, aggregation, and cell differentiation.
Structural control of caspase-generated glutamyl-tRNA synthetase by appended noncatalytic WHEP domains
Halawani, Gogonea, DiDonato et al
J. Biol. Chem. (2018) 293 (23), 8843-8860 DOI: 10.1074/jbc.M117.807503
Halawani, Gogonea, DiDonato et al
J. Biol. Chem. (2018) 293 (23), 8843-8860 DOI: 10.1074/jbc.M117.807503
AI Summary: OriGene’s human EPRS cDNA plasmid (MR218237) was used as the template for generating constructs to study the structural and functional roles of WHEP domains in glutamyl-tRNA synthetase.
Snippet: Cloning and constructs. For bacterial expression, ERS2.5W (corresponding to amino acids 1-929) and PRS0.5W (corresponding to amino acids 930-1512) were subcloned in pTrcHisB (ThermoFisher) by PCR using human EPRS cDNA (Origene plasmid MR218237) as template and the restriction enzyme sites SacI and BstBI for ERS2.5W and BamHI and HindIII for PRS0.5W
Showing 1-2 of 2 papers.
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