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Tusc5 (NM_177709) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR218173
Tusc5 (Myc-DDK-tagged) - Mouse tumor suppressor candidate 5 (Tusc5)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Tusc5 or NM_177709"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Tusc5
Synonyms C130069F04Rik; DSPB1; Lost1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218173 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCAACCCAGCGCAGCCTCCGCTTCAGGACCCAGGCTCCACCTCACCCTTGGAACTGCCTGAGATGG
AGAAACTACTCACAAAAGTGGAGAACAAGGATGACCAAGCCCTGAATCTGTCCAAGTCTCTGTCAGGGGC
TCTGGACCTGGAGCAGAATGGTCACAGCCTGCCCTTCAAGGTCATCTCCGAGGGGCACCGCCAGCCCTCC
CTTTCCGGCTCCCCTTCCCGGGTCAGCTCTCGGCGAGCATCCTCCGTCATCACCACATCCTACGCCCAGG
ACCAAGAAGCCCCCAAAGATTACCTGGTCCTTGCCATTGCCTCTTGCTTCTGCCCCGTCTGGCCCCTCAA
CCTCATCCCCCTTATCTTTTCTATCATGTCTCGAAGTAGTGTGCAGCAGGGGGACCTGGATGGGGCACGG
AGGCTGGGCCGCCTGGCACGGCTACTCAGCATCACCTTCATCATCCTGGGCATCGTTATCATCATTGTGG
CTGTCACTGTCAATTTCACAGTTCCGAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218173 protein sequence
Red=Cloning site Green=Tags(s)

MANPAQPPLQDPGSTSPLELPEMEKLLTKVENKDDQALNLSKSLSGALDLEQNGHSLPFKVISEGHRQPS
LSGSPSRVSSRRASSVITTSYAQDQEAPKDYLVLAIASCFCPVWPLNLIPLIFSIMSRSSVQQGDLDGAR
RLGRLARLLSITFIILGIVIIIVAVTVNFTVPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177709
ORF Size 519 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177709.3, NP_808377.1
RefSeq Size 3237 bp
RefSeq ORF 522 bp
Locus ID 237858
UniProt ID Q8C838
Cytogenetics 11 B5
MW 18.7 kDa
Summary Regulates insulin-mediated adipose tissue glucose uptake and transport by modulation of SLC2A4 recycling. Not required for SLC2A4 membrane fusion upon an initial stimulus, but rather is necessary for proper protein recycling during prolonged insulin stimulation.UniProtKB/Swiss-Prot Function
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Documents
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"NM_177709" in other vectors (4)
SKU Description Size Price
MC213045 Tusc5 (untagged) - Mouse tumor suppressor candidate 5 (Tusc5), (10ug) 10 ug
$330.00
MG218173 Tusc5 (tGFP-tagged) - Mouse tumor suppressor candidate 5 (Tusc5), (10ug) 10 ug
$500.00
MR218173L3 Lenti ORF clone of Tusc5 (Myc-DDK-tagged) - Mouse tumor suppressor candidate 5 (Tusc5) 10 ug
$600.00
MR218173L4 Lenti ORF clone of Tusc5 (mGFP-tagged) - Mouse tumor suppressor candidate 5 (Tusc5) 10 ug
$600.00

Plasmid Map for MR218173

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218173
Circular map for MR218173

file-alt Citations

Reviewer #1 (Public review): PRRT2 as an auxiliary regulator of Nav channel slow inactivation
elife (2026)
AI Summary: OriGene products, specifically the Tmem233 (Cat#: MR214829), Trarg1 (Cat#: MR218173), and Scn1b gene constructs, were used in this study to facilitate gene expression analyses and functional assays investigating PRRT2’s role as an auxiliary regulator of Nav channel slow inactivation.
Snippet: nk 100329612 Gene (M. musculus) Tmem233 OriGene Technologies Cat#:MR214829 Also known as DSPB2 Gene (M. musculus) Trarg1 OriGene Technologies Cat#:MR218173 Also known as DSPB1, Tusc5 Gene (M. musculus) Scn1b OriGene Technologies C
eLife Assessment: PRRT2 as an auxiliary regulator of Nav channel slow inactivation
Swartz
elife (2026)
AI Summary: OriGene products contributed to this study by providing plasmids for mouse genes Tmem233 (Cat#: MR214829), Trarg1 (Cat#: MR218173), and Scn1b, which were used to investigate the molecular regulation of Nav channel slow inactivation.
Snippet: nBank 69017 Gene (Danio rerio) Zebrafish Prrt2 GenBank 100329612 Gene (M. musculus) Tmem233 OriGene Technologies Cat#:MR214829 Also known as DSPB2 Gene (M. musculus) Trarg1 OriGene Technologies Cat#:MR218173 Also known as DSPB1, Tusc5 Gene (M. musculus) Scn1b OriGene
Showing 1-2 of 2 papers.
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