OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Hadhb (NM_145558) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR218102
Hadhb (Myc-DDK-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (Hadhb), nuclear gene enco
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

TA363174 HADHB Antibody - middle region
100 ul
$585.00
TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Hadhb or NM_145558"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Hadhb
Synonyms 4930479F15Rik; Mtpb; TP-beta
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218102 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTACCATCTTGACTTCCACTTTTAGAAACTTGTCAACTACATCAAAATGGGCTCTCAGATCTTCTA
TAAGACCTCTGAGCTGTTCTTCCCAACTGCACTCTGCCCCAGCTGTCCAGACCAAGTCAAAGAAGACTTT
AGCAAAACCCAATATGAAGAATATTGTGGTGGTGGAAGGGGTCCGCATTCCATTTCTGCTGTCAGGCACT
TCGTATAAAGACCTAATGCCACATGACTTGGCTAGAGCTGCACTTTCGGGTTTGTTGCATCGGACCAATA
TTCCAAAGGATGTTGTTGATTATATCATCTTTGGTACAGTTATTCAGGAAGTGAAAACAAGCAATGTGGC
TAGAGAGGCTGCCCTGGGAGCTGGCTTCTCTGATAAGACTCCAGCTCACACTGTCACCATGGCTTGTATC
TCTTCAAACCAAGCCATGACCACAGCTGTTGGTCTGATAGCTTCTGGCCAGTGTGATGTCGTCGTGGCTG
GTGGTGTTGAGTTAATGTCTGATGTCCCTATTCGTCATTCAAGAAATATGAGGAAAATGATGCTTGATCT
CAATAAAGCCAAGACTCTGGGCCAGCGCCTGTCCTTACTCAGTAAATTCAGATTGAATTTTCTGTCCCCT
GAGCTCCCTGCAGTGGCTGAGTTCTCCACTAATGAGACCATGGGCCACTCTGCAGACCGACTGGCTGCTG
CCTTTGCTGTTTCTCGAATGGAACAGGATGAATATGCACTGCGTTCTCATAGTCTGGCCAAGAAGGCACA
GGATGAAGGACACCTTTCTGATATTGTACCCTTCAAAGTACCAGGAAAGGACACAGTTACCAAAGATAAT
GGGATCCGTCCTTCCTCACTGGAGCAAATGGCCAAACTAAAACCTGCGTTCATCAAACCCTATGGCACAG
TGACAGCTGCAAATTCTTCTTTCCTGACTGATGGCGCTTCTGCGATGCTAATCATGTCAGAGGACAGAGC
TCTGGCCATGGGTTATAAACCAAAGGCATATTTGAGGGATTTTATATATGTGTCCCAGGATCCGAAAGAT
CAGCTTTTACTCGGACCAACATATGCTACTCCAAAAGTTTTAGAAAAGGCAGGGTTAACCATGAATGATA
TCGATGCTTTTGAATTTCATGAAGCCTTCTCAGGCCAGATTTTAGCTAACTTTAAAGCCATGGATTCTGA
TTGGTTTGCACAAAACTACATGGGTAGGAAAACCAAGGTTGGATCACCTCCTCTGGAGAAGTTTAATATC
TGGGGCGGATCACTGTCTCTGGGGCACCCTTTTGGAGCCACTGGCTGTCGGCTGGTCATGGCAGCTGCCA
ACAGACTGAGGAAGGATGGAGGCCAGTATGCTTTAGTGGCTGCCTGTGCAGCTGGAGGACAGGGTCATGC
TATGATTGTGGAAGCTTACCCCAAA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR218102 protein sequence
Red=Cloning site Green=Tags(s)

MTTILTSTFRNLSTTSKWALRSSIRPLSCSSQLHSAPAVQTKSKKTLAKPNMKNIVVVEGVRIPFLLSGT
SYKDLMPHDLARAALSGLLHRTNIPKDVVDYIIFGTVIQEVKTSNVAREAALGAGFSDKTPAHTVTMACI
SSNQAMTTAVGLIASGQCDVVVAGGVELMSDVPIRHSRNMRKMMLDLNKAKTLGQRLSLLSKFRLNFLSP
ELPAVAEFSTNETMGHSADRLAAAFAVSRMEQDEYALRSHSLAKKAQDEGHLSDIVPFKVPGKDTVTKDN
GIRPSSLEQMAKLKPAFIKPYGTVTAANSSFLTDGASAMLIMSEDRALAMGYKPKAYLRDFIYVSQDPKD
QLLLGPTYATPKVLEKAGLTMNDIDAFEFHEAFSGQILANFKAMDSDWFAQNYMGRKTKVGSPPLEKFNI
WGGSLSLGHPFGATGCRLVMAAANRLRKDGGQYALVAACAAGGQGHAMIVEAYPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_145558
ORF Size 1425 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_145558.2, NP_663533.1
RefSeq Size 2045 bp
RefSeq ORF 1428 bp
Locus ID 231086
UniProt ID Q99JY0
Cytogenetics 5 B1
MW 51.4 kDa
Summary Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities, while the trifunctional enzyme subunit beta/HADHB described here bears the 3-ketoacyl-CoA thiolase activity.UniProtKB/Swiss-Prot Function
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_145558" in other vectors (4)
SKU Description Size Price
MC216562 Hadhb (untagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (Hadhb), nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$503.00
MG218102 Hadhb (tGFP-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein) beta subunit (Hadhb) nuclear gene encoding mitochondrial protein, (10ug) 10 ug
$657.00
MR218102L3 Lenti ORF clone of Hadhb (Myc-DDK-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (Hadhb), nuclear gene enco 10 ug
$757.00
MR218102L4 Lenti ORF clone of Hadhb (mGFP-tagged) - Mouse hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit (Hadhb), nuclear gene enco 10 ug
$757.00

Plasmid Map for MR218102

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR218102
Circular map for MR218102

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products