Ssty1 (NM_009220) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR218066
Ssty1 (Myc-DDK-tagged) - Mouse spermiogenesis specific transcript on the Y 1 (Ssty1)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ssty1
Synonyms Smy; Ssty
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR218066 representing NM_009220
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCATCCCTCATGAAGAAGAGGAGGAGGAAGTCTTCTTCCAACACCCTGAGGAATATTGTCAGCTGCA
GAATTTCTCACAGTTGGAAGGAAGGTAATGAGCCTGTCACCCAATGGAAGGCCATAGTTCTAGATCAACT
GCCAACAAACCCTTCTCTTTACTTGGTGAAGTATGATGGAATTGACAGCATCTACGTACTGGAGCTCTAC
AGTGATGACAGGATTTTAAACCTTAAGGTTTTGCCTCCCATAGTAGTATTTCCTCAGGTGAGGGATGCCC
ACCTCGCCAGAGCCCTGGTTGGCAGAGCGGTACAACACAAATTTGAGAGGAAAGATGGCTCTGAGGTCAA
CTGGAGGGGGGTGGTGCTAGCCCAGGTGCCAATCATGAAGGATTTGTTTTACATTACCTACAAGAAGGAT
CCAGCTCTCTATGCTTATCAGCTCCTGGATGACTACAAGGAAGGTAACCTCCACATGATTCCAGACACTC
CTCCGGCTGAGGAGAGATCAGGAGATGACAGTGATGTGTTGATTGGTAACTGGGTGGAGTACACCAGAAA
AGATGGTTCCAAAAAGTTCGGAAAGGTTGTTTACCAAGTTCTAGCCAATCCTTCCGTGTACTTTATCAAG
TTTCATGGTGACATCCATATCTATGTCTATACTATGGTGCCAAAGATTCTTGAAGTTGAAAAATCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR218066 representing NM_009220
Red=Cloning site Green=Tags(s)

MSSLMKKRRRKSSSNTLRNIVSCRISHSWKEGNEPVTQWKAIVLDQLPTNPSLYLVKYDGIDSIYVLELY
SDDRILNLKVLPPIVVFPQVRDAHLARALVGRAVQHKFERKDGSEVNWRGVVLAQVPIMKDLFYITYKKD
PALYAYQLLDDYKEGNLHMIPDTPPAEERSGDDSDVLIGNWVEYTRKDGSKKFGKVVYQVLANPSVYFIK
FHGDIHIYVYTMVPKILEVEKS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009220
ORF Size 696 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009220.2, NP_033246.2
RefSeq Size 1285 bp
RefSeq ORF 699 bp
Locus ID 20611
UniProt ID P13675
Cytogenetics Y A1
MW 26.8 kDa
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Documents
Resources
"NM_009220" in other vectors (4)
SKU Description Size Price
MC209427 Ssty1 (untagged) - Mouse spermiogenesis specific transcript on the Y 1 (Ssty1), (10ug) 10 ug
$330.00
MG218066 Ssty1 (tGFP-tagged) - Mouse spermiogenesis specific transcript on the Y 1 (Ssty1), (10ug) 10 ug
$500.00
MR218066L3 Lenti ORF clone of Ssty1 (Myc-DDK-tagged) - Mouse spermiogenesis specific transcript on the Y 1 (Ssty1) 10 ug
$600.00
MR218066L4 Lenti ORF clone of Ssty1 (mGFP-tagged) - Mouse spermiogenesis specific transcript on the Y 1 (Ssty1) 10 ug
$600.00

Plasmid Map for MR218066

Download sequence for

Cloning scheme for MR218066

Circular map for MR218066

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