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Mrs2 (NM_001013389) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217956
Mrs2 (Myc-DDK-tagged) - Mouse MRS2 magnesium homeostasis factor homolog (S. cerevisiae) (Mrs2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Mrs2
Synonyms Gm902; HPT; Mrs2l; RPT
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217956 representing NM_001013389
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATGCCTGCGCTGCCTGCCCGGGCTGCTGCCCCGCGCCGCGCAGCCCCGGAGGGCCCTGTGGACGG
CGGTCGCGCGCCTGAGCCTCGCTGCCTGCGGCGGCCGCGCGACCCCTTTGCGCTCCCGGAGCCCGAAAGC
GTCGAGCACAGCCCGCGCTGCAGGTGATGTGCTCCGGTTTAGAACCTCGGATGCCTCTCAAGCCACTTTA
GCCAGCGTGGCCCAGGTGTTTGCTGTGACAAAGTTTGACAAAGAAGGAAACGTTACCTCTTTTGAGAGGA
AGAAAACTGAACTATACCATGAGTTAGCACTCCAGGCCAGAGACTTGAGGTTTCAGCACGTGATGAGCAT
CACTACTAGAAACAACAGGATCATCATGAGGATGGAGTATTTGAAGGCTGTGATAACTCCAGAGTGTCTT
CTGATACTAGATTATCGGAATTTAAATTTGGAGCACTGGCTGTTTCGGGAACTGCCCTCACAGCTGGCTG
GAGAAGGTCAGCTGGTCACCTACCCATTACCATTTGAGTTCAGAGCTATTGAAGCACTCCTGCAGTACTG
GATCAGCACCCTTCGGGGGAGACTAAGCGTCCTGCAGCCACTGATCCTCGAGACCCTGGATGCTCTAGTG
GACCCCAAGCACTCGTCTGTGGACAGAAGCAAGCTGCATGTCCTACTCCAAAACGGCAAAAGTCTCTCTG
AGTTAGAAACAGACATTAAAATTTTCAAAGAGTCGATTTTGGAGCTCTTGGATGAGGAGGAGATGCTGGA
AGAACTCTGTCTGACCAAGTGGAGCGACCCACATGTCTTTGAGAAGAGCAGCACTGGGATCGACCACGCA
GAGGAGATGGAGCTGCTGCTGGAGAACTACTACCGCTTAGCAGAGGACCTCTCCAACGAGGCGCGCGAGC
TTCGGGTCCTGATTGACGATTCGCAGAGCATTATCTTCATCAACCTAGACAGCCATCGCAATGTGATGAT
GAGGTTAAATCTCCAGCTGACCATGGGAACCTTCTCCCTTTCACTCTTTGGTCTGATGGGAGTTGCTTTT
GGCATGAATTTGGAATCTTCCCTTGAAGAGGACCATCGCGTGTTTTGGCTGGTTACGGGGATTATGTTTA
TGGGAAGTGGTCTCATCTGGAGGAGGTTGCTCTCATTCCTTGGAAGACAGCTGGAAGCTCCTGTGCCCCC
TGTGATGACCTCCTTACCTAAAAAGACCCTTCTGGCCAATAGAAGGATGGACGTGAAAAACAGCCTCAGG
CCGGAAGGACTTGGAGCAAGCAGGACTATCCTGGCAAGCCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217956 representing NM_001013389
Red=Cloning site Green=Tags(s)

MECLRCLPGLLPRAAQPRRALWTAVARLSLAACGGRATPLRSRSPKASSTARAAGDVLRFRTSDASQATL
ASVAQVFAVTKFDKEGNVTSFERKKTELYHELALQARDLRFQHVMSITTRNNRIIMRMEYLKAVITPECL
LILDYRNLNLEHWLFRELPSQLAGEGQLVTYPLPFEFRAIEALLQYWISTLRGRLSVLQPLILETLDALV
DPKHSSVDRSKLHVLLQNGKSLSELETDIKIFKESILELLDEEEMLEELCLTKWSDPHVFEKSSTGIDHA
EEMELLLENYYRLAEDLSNEARELRVLIDDSQSIIFINLDSHRNVMMRLNLQLTMGTFSLSLFGLMGVAF
GMNLESSLEEDHRVFWLVTGIMFMGSGLIWRRLLSFLGRQLEAPVPPVMTSLPKKTLLANRRMDVKNSLR
PEGLGASRTILASR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001013389
ORF Size 1302 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001013389.2, NP_001013407.2
RefSeq Size 2035 bp
RefSeq ORF 1305 bp
Locus ID 380836
UniProt ID Q5NCE8
Cytogenetics 13 A3.1
MW 49.7 kDa
Summary Magnesium transporter that mediates the influx of magnesium into the mitochondrial matrix. Required for normal expression of the mitochondrial respiratory complex I subunits.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001013389" in other vectors (4)
SKU Description Size Price
MC216752 Mrs2 (untagged) - Mouse MRS2 magnesium homeostasis factor homolog (S. cerevisiae) (Mrs2), (10ug) 10 ug
$503.00
MG217956 Mrs2 (tGFP-tagged) - Mouse MRS2 magnesium homeostasis factor homolog (S. cerevisiae) (Mrs2), (10ug) 10 ug
$657.00
MR217956L3 Lenti ORF clone of Mrs2 (Myc-DDK-tagged) - Mouse MRS2 magnesium homeostasis factor homolog (S. cerevisiae) (Mrs2) 10 ug
$757.00
MR217956L4 Lenti ORF clone of Mrs2 (mGFP-tagged) - Mouse MRS2 magnesium homeostasis factor homolog (S. cerevisiae) (Mrs2) 10 ug
$757.00

Plasmid Map for MR217956

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217956
Circular map for MR217956

file-alt Citations

The Role of Lactate as an Endogenous Ligand for Intracellular Magnesium Release and Mitochondrial Magnesium Channel Activation to Integrate Cellular Metabolism
Daw
Thesis (2021)
Snippet: promoter. Ad-Mrs2.mRFP.FLAG was generated using the cDNA from mouse Mrs2 ORF clone (Origene;#MR217956). The Ad-Mrs2(mut).mRFP.FLAG was generated by mutating the ORF to encode the following mutant protein sequence at aa#396-406: "IKLLKSSLKKK
Lactate Elicits ER-Mitochondrial Mg2+ Dynamics to Integrate Cellular Metabolism
Daw, Ramachandran, Enslow et al
Cell (2020) 183 (2), 474-489.e17 DOI: 10.1016/j.cell.2020.08.049
Cited by 220
AI Summary: In this study, OriGene's Mouse Mrs2 ORF Clone (Cat# MR217956) and a mutant Mouse Mrs2 ORF Clone (derived from Cat# MR217956) were used to investigate the role of Mrs2 in mitochondrial Mg2+ uptake during lactate-induced ER-mitochondrial Mg2+ dynamics.
Snippet: pCMV MagFRET1 Lindenburg et al., 2013 Addgene Plasmid 50742 Mouse Mrs2 ORF Clone OriGene Cat# MR217956 Mouse Mrs2(mut aa396-406) ORF Clone (Derived From Cat# MR217956) OriGene N/A pGCamp6-mt Nemani et al., 2018 N/A Software and Algorithms GraphPad Prism version 8 GraphPad Software https://www.graphpad.com/scientific-software/prism/
Figure
Showing 1-2 of 2 papers.
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