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Ebf4 (NM_001110513) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217870
Ebf4 (Myc-DDK-tagged) - Mouse early B-cell factor 4 (Ebf4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $558.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "Ebf4 or NM_001110513"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ebf4
Synonyms Ebf3; O/E-4; Olf-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217870 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTCCCCGCACAGGACGCTCTGCCCCGCGGTGGGCTCCATCTGAAAGAGGAGCCGCTGCTGCCCTCCA
GCCTGGGCTCGGTGCGCTCCTGGATGCAGAGCGCGGGCATCCTGGACTCCAACACCGCGGCGCAGAGTGG
CGTGGGCCTGGCAAGAGCACATTTTGAAAAGCAGCCCCCCTCCAACCTCAGGAAGTCCAACTTCTTCCAC
TTCGTGCTGGCCATGTACGACCGGCAGGGGCAGCCCGTGGAGGTGGAGCGCACAGCCTTCATCGACTTCG
TGGAAAAGGATCGAGAGCCTGGGACGGAAAAGACAAACAACGGGATCCATTACCGCCTTCGGCTGGTGTA
TAATAATGGACTTCGGACAGAGCAAGACCTCTACGTGCGCCTTATTGACTCCATGTCGAAGCAGGCTATC
ATCTATGAGGGACAGGACAAGAATCCTGAGATGTGCCGAGTGCTGCTCACTCATGAGATCATGTGCAGCC
GGTGCTGTGACCGCAAGAGCTGTGGTAACCGGAATGAGACGCCTTCTGATCCAGTCATTATTGACAGGTT
CTTCCTCAAATTCTTTCTCAAATGCAACCAGAACTGCTTGAAGAATGCCGGGAATCCCCGAGACATGCGC
CGCTTCCAGGTGGTGGTGTCCACGACCGTGAGTGTGGATGGCCATGTGCTGGCTGTGTCGGACAACATGT
TTGTGCACAACAACTCCAAGCATGGACGCAGAGCCCGACGCCTGGACCCTTCTGAAGCTGCCACCCCCTG
CATCAAGGCCATCAGCCCAGGGGAGGGCTGGACCACGGGAGGCGCCACCGTCATTATCATCGGAGACAAC
TTCTTCGACGGGTTGCAGGTCGTGTTTGGCAACGTGCTCCTGTGGAGCGAGCTCATCACTCCCCATGCTA
TCCGGGTGCAGACACCACCCCGACACATCCCTGGGGTAGTAGAAGTGACCCTCTCCTACAAGTCCAAGCA
GTTCTGTAAAGGAGCCCCTGGCCGCTTTGTCTACACAGCCCTGAATGAACCCACCATTGACTACGGATTC
CAGAGGCTCCAGAAAGTTATTCCCAGACACCCCGGAGACCCTGAGAGGCTACCCAAGGAAGTGCTGCTGA
AAAGGGCGGCTGACTTGGCGGAGGCCCTGTACGGAGTGCCCAGCAGTAACCAGGAGCTCCTCCTGAAACG
TGCGGCGGACGTGGCCGAGGCTCTGTACAGCGCCCCGCGCGCGCCTGCACCACTCGGACCCCTGGCCCCC
AGCCACCCGCACCCCGCCGTCGTGGGTATCAATGCCTTCAGCAGCCCTCTGGCCATCGCCGTCGGGGATA
CCACACCGGAGCCGGGCTATGCCCGTAGCTGCGGCAGCGCGTCTCCCCGGTTCGCGCCCAGCCCCGGCTC
TCAGCAGAGCAGCTATGGCAGTGGCCTTGGAGCCGGCCTCGGTAGCTATGGCGCGCCTGGTGTGACTGGC
CTTGGCGTGCCCGGGTCCCCTAGCTTCCTCAATGGCTCCACCGCAACCTCACCTTTCGCCATCATGCCCT
CTAGCCCCCCGCTGGCGGCTGCCTCCTCCATGTCCCTTCCGGCCGCCGCCCCCACCACCAGCGTCTTCTC
CTTCTCGCCTGTCAACATGATCTGCGCTGTCAAGCAGAGGAGCGCCTTCGCCCCTGTGCTGCGCCCACCC
AGTTCCCCATCGCAGGCCTGCCCCAGAGCCCACAGAGAGGGGCTTCCAGACCAGCCTTTTGAAGATACTG
ACAAGTTCCACTCTGCAGCCCGGGGACTCCAGGGCTTGGCATACTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217870 protein sequence
Red=Cloning site Green=Tags(s)

MFPAQDALPRGGLHLKEEPLLPSSLGSVRSWMQSAGILDSNTAAQSGVGLARAHFEKQPPSNLRKSNFFH
FVLAMYDRQGQPVEVERTAFIDFVEKDREPGTEKTNNGIHYRLRLVYNNGLRTEQDLYVRLIDSMSKQAI
IYEGQDKNPEMCRVLLTHEIMCSRCCDRKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKNAGNPRDMR
RFQVVVSTTVSVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEAATPCIKAISPGEGWTTGGATVIIIGDN
FFDGLQVVFGNVLLWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFVYTALNEPTIDYGF
QRLQKVIPRHPGDPERLPKEVLLKRAADLAEALYGVPSSNQELLLKRAADVAEALYSAPRAPAPLGPLAP
SHPHPAVVGINAFSSPLAIAVGDTTPEPGYARSCGSASPRFAPSPGSQQSSYGSGLGAGLGSYGAPGVTG
LGVPGSPSFLNGSTATSPFAIMPSSPPLAAASSMSLPAAAPTTSVFSFSPVNMICAVKQRSAFAPVLRPP
SSPSQACPRAHREGLPDQPFEDTDKFHSAARGLQGLAYS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001110513
ORF Size 1797 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001110513.1, NP_001103983.1
RefSeq Size 2687 bp
RefSeq ORF 1800 bp
Locus ID 228598
UniProt ID Q8K4J2
Cytogenetics 2 F1
MW 64.6 kDa
Summary Seems to weakly activate transcription. Binds an Olf-1 consensus site in vitro.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001110513" in other vectors (4)
SKU Description Size Price
MC219571 Ebf4 (untagged) - Mouse early B-cell factor 4 (Ebf4), (10ug) 10 ug
$614.00
MG217870 Ebf4 (tGFP-tagged) - Mouse early B-cell factor 4 (Ebf4), (10ug) 10 ug
$758.00
MR217870L3 Lenti ORF clone of Ebf4 (Myc-DDK-tagged) - Mouse early B-cell factor 4 (Ebf4) 10 ug
$858.00
MR217870L4 Lenti ORF clone of Ebf4 (mGFP-tagged) - Mouse early B-cell factor 4 (Ebf4) 10 ug
$858.00

Plasmid Map for MR217870

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217870
Circular map for MR217870

file-alt Citations

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