St6galnac6 (NM_016973) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR217869
St6galnac6 (Myc-DDK-tagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6), transcript variant 1
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol St6galnac6
Synonyms Siat7f; ST6GalNAcVI
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217869 representing NM_016973
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTGCTCGAGGCCCCCCAGCCAGTGTGACCCCACAACCCTGCCCCCCGGGCCACCTGCCGGACGCT
GGCCTCTACCCTTCAGCAGACGCCGGAGAGAGATGAGTAGCAACAAAGAGCAGCGGTCAGCAGTGTTTGT
GATCCTCTTTGCCCTCATCACCATCCTCATCCTCTACAGCTCCAACAGTGCCAACGAGGTCTTCCACTAC
GGCTCCCTGCGGGGCCGCACGCGTCGGCCAGTCAACCTCAAGAAGTGGAGTTTCTCCAGCGCCTACTTCC
CTATCCTCGGCAACAAGACGCTGCCGTCCAGGTGCAACCAGTGTGTGATCATCACCAGCTCCAGCCACCT
GCTGGGCACCAAACTGGGCCCTGAGATTGAGCGGGCTGAGTGCACCATCCGCATGAACGATGCTCCCACC
TCTGGCTACTCGGCTGACGTCGGAAACAAAACCACCTTCCGCGTAGTGGCCCATTCCAGTGTATTCCGTG
TGCTGCGGAAGCCCCAGGAATTTGTCAACCGGACCCCTGAGACGGTGTTCATCTTCTGGGGACCCCCAAA
CAAGATGCAGAAGCCCCAGGGCAGCCTCCTGCGCGTCATCCAGCGGGCGGGCCTCATGTTCCCGAACATG
GAGGCCTATGCCGTCTCTCCCGCCCGCATGCAGCAGTTTGACGACCTCTTCCGGGGTGAGACGGGCAAGG
ACAGGGAAAAGTCCCATTCCTGGTTGAGCACGGGCTGGTTTACCATGGTGATTGCGGTGGAATTGTGTGA
CCATGTGCACGTGTATGGCATGGTCCCTCCTGACTACTGCAGCCAGCGGCCCCGCCTGCAGCGCATGCCA
TACCACTACTATGAACCCAAGGGGCCGGACGAGTGTGTCACCTACATCCAGAACGAGCACAGCCGTAAGG
GCAATCACCACCGCTTCATCACCGAGAAGAGGGTCTTCTCGTCCTGGGCCCAACTCTACGGTATCACCTT
CTCCCACCCCTCCTGGACC


AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
TGGATTACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR217869 representing NM_016973
Red=Cloning site Green=Tags(s)

MACSRPPSQCDPTTLPPGPPAGRWPLPFSRRRREMSSNKEQRSAVFVILFALITILILYSSNSANEVFHY
GSLRGRTRRPVNLKKWSFSSAYFPILGNKTLPSRCNQCVIITSSSHLLGTKLGPEIERAECTIRMNDAPT
SGYSADVGNKTTFRVVAHSSVFRVLRKPQEFVNRTPETVFIFWGPPNKMQKPQGSLLRVIQRAGLMFPNM
EAYAVSPARMQQFDDLFRGETGKDREKSHSWLSTGWFTMVIAVELCDHVHVYGMVPPDYCSQRPRLQRMP
YHYYEPKGPDECVTYIQNEHSRKGNHHRFITEKRVFSSWAQLYGITFSHPSWT

SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-RsrII Cloning Scheme for this gene | Plasmid Map
ACCN NM_016973
ORF Size 999 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016973.3, NP_058669.1
RefSeq Size 2457 bp
RefSeq ORF 1002 bp
Locus ID 50935
UniProt ID Q9JM95
Cytogenetics 2 B
MW 38.6 kDa
Summary Alpha-2,6-sialyltransferase involved in the synthesis of alpha-series gangliosides. Has activity toward GD1a, GT1b and GM1b. Has no activity toward glycoproteins. Responsible for the biosynthesis of DSGG (disialylgalactosylgloboside) from MSGG (monosialylgalactosylgloboside) in kidney.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_016973" in other vectors (4)
SKU Description Size Price
MC209884 St6galnac6 (untagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6), transcript variant 1, (10ug) 10 ug
$503.00
MG217869 St6galnac6 (tGFP-tagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-23-beta-galactosyl-13)-N-acetylgalactosaminide alpha-26-sialyltransferase 6 (St6galnac6) transcript variant 1, (10ug) 10 ug
$500.00
MR217869L3 Lenti ORF clone of St6galnac6 (Myc-DDK-tagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6), transcript variant 1 10 ug
$600.00
MR217869L4 Lenti ORF clone of St6galnac6 (mGFP-tagged) - Mouse ST6 (alpha-N-acetyl-neuraminyl-2,3-beta-galactosyl-1,3)-N-acetylgalactosaminide alpha-2,6-sialyltransferase 6 (St6galnac6), transcript variant 1 10 ug
$600.00

Plasmid Map for MR217869

Download sequence for

Cloning scheme for MR217869

Circular map for MR217869

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