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Krt33b (NM_013570) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217680
Krt33b (Myc-DDK-tagged) - Mouse keratin 33B (Krt33b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Krt33b
Synonyms Ha3; Ha4; Krt1-3; mHa3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217680 representing NM_013570
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCATACAACTGCTGCCTGCCCGCCATGAGCTGCCGCACCAGCTGCTCTTCCCGGCCCTGCGTGCCCC
CCAGCTGCCATGGCTGCACCCTGCCCGGGGCCTGCAACATCCCCGCCAATGTGGGCAACTGCAATTGGTT
CTGTGAGGGCTCCTTCAATGGCAATGAGAGGGAGACCATGCAGTTCCTGAATGACCGCCTGGCCTCCTAT
ATGGAGAAGGTGAGGCAGCTGGAGAGAGAGAATGCAGAGCTGGAATGTAAGATCCAAGAGAGGAACCAGC
AGCAGGACCCCCTGGTGTGTCCTGCCTACCAGGCCTACTTCAGGACCATTGAGGAGCTGCAGCAGAAGAT
TCTCTGTGGCAAGTCTGAGAACGGCAGGCTGGTGGTGCAGATCGACAATGCCAAGCTGGCTGCTGATGAC
TTCAGGACCAAGTATGAGTCAGAGCTGTCCCTGCGGCAGCTGGTGGAGTCAGACCTCAATGGCCTGCGCA
GGATCCTGGACGAACTGACCCTCTGCAAGTCTGATCTGGAGGCACAGGTGGAGTCCCTGAGGCAGGAGCT
GCTGTGTCTCAAGCAAAACCATGAGGAGGAAGTCAACACCCTGCGCTGCCAGCTTGGAGACCGCCTCAAC
GTAGAGGTGGACGCTGCTCCCACCGTGGACCTGAACCGAGTGCTCAACGAGACCAGGTGTCAGTACGAGG
CCCTGGTGGAAACCAACCGCCGGGAAGTGGAGGAATGGTTCACCACACAGACAGAGGAGCTGAACAAGCA
GGTGGTGTCTAGCTCAGAGCAGCTGCAGTCCTGCCAGGCAGAGATCATCGAGCTGAGACGCACAGTCAAT
GCCCTGGAGATCGAGCTGCAGGCCCAGCACAGCATGAGAAACTCTCTGGAGAATACCCTGGCAGAGAGTG
AGGCTCGCTACAGCTCTCAGCTGTCCCAGGTGCAGTGCCTGATCACCAACGTGGAGTCCCAGCTTGGTGA
GATCCGGGCTGACCTGGAGCGTCAGAACCAGGAGTACCAGGTGTTGCTGGATGTCAAGGCCCGGCTGGAG
TGTGAGATCAACACGTATAGGGGCCTGCTGGAGAGCGAGGACTGCAAGCTGCCCTGCAACCCCTGTGCCA
CCACCAATGCCTGTGACAAGCCCATTGGACCCTGTGTCACAAACCCTTGTGTCACACGCTCCCGATGTGG
ACCCTGCAATTCCTTTGGCTGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217680 representing NM_013570
Red=Cloning site Green=Tags(s)

MPYNCCLPAMSCRTSCSSRPCVPPSCHGCTLPGACNIPANVGNCNWFCEGSFNGNERETMQFLNDRLASY
MEKVRQLERENAELECKIQERNQQQDPLVCPAYQAYFRTIEELQQKILCGKSENGRLVVQIDNAKLAADD
FRTKYESELSLRQLVESDLNGLRRILDELTLCKSDLEAQVESLRQELLCLKQNHEEEVNTLRCQLGDRLN
VEVDAAPTVDLNRVLNETRCQYEALVETNRREVEEWFTTQTEELNKQVVSSSEQLQSCQAEIIELRRTVN
ALEIELQAQHSMRNSLENTLAESEARYSSQLSQVQCLITNVESQLGEIRADLERQNQEYQVLLDVKARLE
CEINTYRGLLESEDCKLPCNPCATTNACDKPIGPCVTNPCVTRSRCGPCNSFGC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013570
ORF Size 1212 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013570.1, NP_038598.1
RefSeq Size 1596 bp
RefSeq ORF 1215 bp
Locus ID 16671
UniProt ID Q61897
Cytogenetics 11 63.37 cM
MW 45.9 kDa
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Documents
Resources
"NM_013570" in other vectors (4)
SKU Description Size Price
MC208851 Krt33b (untagged) - Mouse keratin 33B (Krt33b), (10ug) 10 ug
$503.00
MG217680 Krt33b (tGFP-tagged) - Mouse keratin 33B (Krt33b), (10ug) 10 ug
$657.00
MR217680L3 Lenti ORF clone of Krt33b (Myc-DDK-tagged) - Mouse keratin 33B (Krt33b) 10 ug
$757.00
MR217680L4 Lenti ORF clone of Krt33b (mGFP-tagged) - Mouse keratin 33B (Krt33b) 10 ug
$757.00

Plasmid Map for MR217680

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217680
Circular map for MR217680

file-alt Citations

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