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Ankrd53 (NM_029245) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217605
Ankrd53 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 53 (Ankrd53)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ankrd53
Synonyms 4930564N15Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217605 representing NM_029245
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGACGCCCCTCGCGGCGTCGTTCCAAAGCCAGTACACCCCCTAGGAGCCACACCACTCCGCGCAGGA
CTGGGCCCTCGGATTCCAGAAGGCGCCCAGGGACAAAAGAGCAGCCCCGCCCATCTGTACAAGGAGGCAC
GCGGCAAGCGGAACATGACTTGAAGGTTTCCAGCCCCAACTCCGAATCCTCGCAGTACAGTACCTCCGAG
TTGTGGAGCCATAAAGTGATCGCGAACTACTCCGAGCTTTTCGCAGCCTCAGTGGGCAATGTGGACTGGC
TTCGGTTCTGTGTGAATCCGGAGCGCAAGGAGATCATAGTCGACGACAAGGGCTTCACAGCCATCCACTT
CGCAGCCCAGAAATGCCAGCTTTCCTGCCTTAAGGTCTTAATAGAGGAGTACAAATATCCCGTGGATCTG
CCCACCAACAAAGGCCAGACGCCCCTGCACTTGGTCATCCATAAAAACAACAAATCGGACATCCTCCCCT
GCATTGACTACCTGCTCAAGAAAGGGGCAGCCATCAACTCGCAGACATACAATGGCTCCACACCCCTGCA
CCTGGCTTCCTGCAATGGCCTGCTGGGATGCATTAAGCTGCTGGTGCAGAGTGGAGCCAATGTGCATGCC
CGAGATGCCACGGGCTTCAAGCCCATCGACTATTGCAGACTTTGGAACCACCGTACCTGTGCCCGGTTCT
TGAAGGATGTCATGTGGAAACATGATAAGAAGGTCCTTGCCCAGGAGATGGAGAAACTGAGGACCTTGAA
GGAGAAGCTGACCATTTTGGAGTATCACTACCTGGTTGAATATCAAAAAGAACATCAAATTCTGAGAGAG
GCTCATTTCAGAAAGTGGCTCCAAAACAAGGTGCTGGCCCAGACCCTGGGTTCTGCCGACTCCAAGCAAA
AGGCTGGTGTCCAACCTTGGTCATTGGCCTCCAACACTTTAAGATGCCCAATAACTGAAAGCCTCCATTA
TCCATCTGTGGAAGCTCAGCTGAAAAACTTGCCCTCACCTGTGGTGCCACCCAAGCCCATCTACAAGCAG
ACCACCATCAGTCGGCCCAAGTTATGGAACTATAGTGCTAACCCTGCCAGGTCCCCCATCACAAACATTG
GCCACCCACAGAATATTCGCCTGGGTGTGCACCCAGAACCCTATAAGGAACATGACTTCCGCAGATTCCT
GGAGGTGACTCGAAATAAACATGGGGGTGCATGCCTGCGCACAGTGGACCGTCAGTTGGTGACACCTGTA
CCCCAGCTGCCTTTTGAGATGATGGTCCGAGTGCTGTACCCAGGGACTCAACCCTACAGGATGAAAGTGC
CCCAGGGCTTGTATCCACGGGACATACTCAAAGTACCTGAGAAACGGCATGTAGGAGATACTTGTAGCAA
TACAATGGCCATGACCCTACGTGAGACATTTGATAAACCCTTCCTCGATTCCCTGGAAGTTTGCCGGACT
CGAGTAGCCCCACCCTCCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217605 representing NM_029245
Red=Cloning site Green=Tags(s)

MRRPSRRRSKASTPPRSHTTPRRTGPSDSRRRPGTKEQPRPSVQGGTRQAEHDLKVSSPNSESSQYSTSE
LWSHKVIANYSELFAASVGNVDWLRFCVNPERKEIIVDDKGFTAIHFAAQKCQLSCLKVLIEEYKYPVDL
PTNKGQTPLHLVIHKNNKSDILPCIDYLLKKGAAINSQTYNGSTPLHLASCNGLLGCIKLLVQSGANVHA
RDATGFKPIDYCRLWNHRTCARFLKDVMWKHDKKVLAQEMEKLRTLKEKLTILEYHYLVEYQKEHQILRE
AHFRKWLQNKVLAQTLGSADSKQKAGVQPWSLASNTLRCPITESLHYPSVEAQLKNLPSPVVPPKPIYKQ
TTISRPKLWNYSANPARSPITNIGHPQNIRLGVHPEPYKEHDFRRFLEVTRNKHGGACLRTVDRQLVTPV
PQLPFEMMVRVLYPGTQPYRMKVPQGLYPRDILKVPEKRHVGDTCSNTMAMTLRETFDKPFLDSLEVCRT
RVAPPSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_029245
ORF Size 1491 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_029245.3, NP_083521.2
RefSeq Size 1737 bp
RefSeq ORF 1494 bp
Locus ID 75305
UniProt ID Q3V0J4
Cytogenetics 6
MW 56.7 kDa
Summary Required for normal progression through mitosis. Involved in chromosome alignment and cytokinesis via regulation of microtubules polymerization.UniProtKB/Swiss-Prot Function
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"NM_029245" in other vectors (4)
SKU Description Size Price
MC217027 Ankrd53 (untagged) - Mouse ankyrin repeat domain 53 (Ankrd53), (10ug) 10 ug
$503.00
MG217605 Ankrd53 (tGFP-tagged) - Mouse ankyrin repeat domain 53 (Ankrd53), (10ug) 10 ug
$657.00
MR217605L3 Lenti ORF clone of Ankrd53 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 53 (Ankrd53) 10 ug
$757.00
MR217605L4 Lenti ORF clone of Ankrd53 (mGFP-tagged) - Mouse ankyrin repeat domain 53 (Ankrd53) 10 ug
$757.00

Plasmid Map for MR217605

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217605
Circular map for MR217605

file-alt Citations

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