Serhl (NM_023475) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR217576
Serhl (Myc-DDK-tagged) - Mouse serine hydrolase-like (Serhl)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Serhl
Synonyms 0610008B10Rik; 1110019M09Rik; AU019786
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217576 representing NM_023475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTTGCACTCAGAGTTGAAGTTGGCTGTGCCCTGGGGCCACATTGCTCTCAAAGTCTGGGGCTCCC
AGAAGAACCCTCCAGTTCTCTGCTTACACGGCTGGCTGGACAACGCCAACTCCTTTGACAGGCTCATTCC
TCTTCTTCCCCAAGACTTCTGTTACATGGCTATGGATTTCGGAGGCCACGGGCTCTCGTCCCATTACAAC
CCAGGCCTCCCGTACTACCAGCAAAATTTTGTGAGTGAGGTCCGAAGGGTGGCAACAGCCTTTAAGTGGA
ATCAGTTCACCCTCCTGGGCCACAGTTTTGGTGGCTGTGTGGGTGGCACGTTCGCCTGCATGTTCCCTGA
GATGGTGGACAAACTGATCTTGCTGGACTCAACGCCATTTTTCCTGGACTCTAATGAAATGGAGAACATA
CTGACCTACAGGCGGAGAAACATAGAGCACACACTTCAGGTGGAGGCCTCCCAGAAAAAGTCCCTGAGAG
CTGTCAGCCCAGAGGAGATGTTGCAGGGGTTCCTGAATAACAACAGCCACTTGGATAAAGACTGTGGGGA
ACTCATCCTACAGAGAGGAACCACGAAGGTGGACGCAGGTCTGGTGCTGAACAGGGACCGGAGGATCTCT
TGGCCAGAGAACAGCTTTGACTTCGTAAGCAAGGAGATGTTCGTGCACTCTGCGAAGAGCCTGCAGGCCA
GCGTGCTAATGATCAAAGCGCTGCAGGGGTACTACGATGTGAGGCGGGCGAATGATGCAGACAAGGCGCC
TATGCACTTCATGGTGGACACACTAAGATCCACCCTAAAGGAGCGGTTCCAGTTTGTGGAAGTCCCAGGC
AATCACTACATCCACATGAACAAGCCCCAGGTGGTGGCCGGAGTCGTCGGTCCCTTCCTACAGGGCCTAC
AGAGGATGACTTCTGCCAGGCTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR217576 representing NM_023475
Red=Cloning site Green=Tags(s)

MGLHSELKLAVPWGHIALKVWGSQKNPPVLCLHGWLDNANSFDRLIPLLPQDFCYMAMDFGGHGLSSHYN
PGLPYYQQNFVSEVRRVATAFKWNQFTLLGHSFGGCVGGTFACMFPEMVDKLILLDSTPFFLDSNEMENI
LTYRRRNIEHTLQVEASQKKSLRAVSPEEMLQGFLNNNSHLDKDCGELILQRGTTKVDAGLVLNRDRRIS
WPENSFDFVSKEMFVHSAKSLQASVLMIKALQGYYDVRRANDADKAPMHFMVDTLRSTLKERFQFVEVPG
NHYIHMNKPQVVAGVVGPFLQGLQRMTSARL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_023475
ORF Size 933 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_023475.3, NP_075964.1
RefSeq Size 1391 bp
RefSeq ORF 936 bp
Locus ID 68607
UniProt ID Q9EPB5
Cytogenetics 15 E1
MW 35.3 kDa
Summary Probable serine hydrolase. May be related to cell muscle hypertrophy.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_023475" in other vectors (4)
SKU Description Size Price
MC210761 Serhl (untagged) - Mouse serine hydrolase-like (Serhl), (10ug) 10 ug
$330.00
MG217576 Serhl (tGFP-tagged) - Mouse serine hydrolase-like (Serhl), (10ug) 10 ug
$500.00
MR217576L3 Lenti ORF clone of Serhl (Myc-DDK-tagged) - Mouse serine hydrolase-like (Serhl) 10 ug
$600.00
MR217576L4 Lenti ORF clone of Serhl (mGFP-tagged) - Mouse serine hydrolase-like (Serhl) 10 ug
$600.00

Plasmid Map for MR217576

Download sequence for

Cloning scheme for MR217576

Circular map for MR217576

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