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Pmepa1 (NM_022995) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217531
Pmepa1 (Myc-DDK-tagged) - Mouse prostate transmembrane protein, androgen induced 1 (Pmepa1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pmepa1
Synonyms 2210418I02Rik; AW455466; Erg1.2; N4wbp4; Stag1; Tmepai
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217531 representing NM_022995
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGGTCAACGGCACCGCCGCCGCCGCCGCCGGGCAGCCCAATGTCTCCTGCGCGTGCAACTGCCAGC
GCTCTTTGTTCCCCAGCATGGAGATCACGGAGCTGGAGTTCGTGCAAATCGTGGTCATCGTGGTAGTGAT
GATGGTGATGGTGGTTATGATTACGTGCCTGCTGAGCCACTACAAGCTGTCAGCCCGCTCCTTCATCAGC
CGACACAGCCAGGCCAGGAGGAGAGACGATGGACTGTCCTCGGAAGGATGCCTCTGGCCCTCAGAGAGTA
CGGTGTCAGGTGGAATGCCGGAGCCACAGGTCTATGCCCCGCCTCGGCCCACTGACCGACTCGCTGTGCC
CCCCTTCATCCAGCGGAGCCGATTCCAACCCACCTACCCCTACCTGCAGCACGAAATTGCCCTGCCACCC
ACCATCTCACTGTCTGATGGGGAGGAGCCCCCACCCTACCAGGGCCCCTGCACCCTCCAGCTACGGGACC
CTGAGCAACAGCTGGAGCTGAACCGGGAATCTGTGCGCGCACCCCCTAACCGGACCATCTTCGACAGTGA
CCTTATAGACAGCACCATGCTGGGGGGCCCCTGTCCCCCCAGCAGTAACTCGGGCATCAGCGCCACCTGC
TACAGCAGCGGTGGGCGCATGGAGGGGCCGCCCCCCACCTACAGCGAGGTCATTGGCCACTACCCTGGCT
CCTCCTTCCAGCACCAGCAAAGTAACGGGCCATCCTCCCTGCTAGAGGGGACCCGGCTCCATCACTCGCA
CATTGCCCCACTGGAGAACAAGGAGAAGGAGAAACAGAAAGGTCACCCCCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217531 representing NM_022995
Red=Cloning site Green=Tags(s)

MGVNGTAAAAAGQPNVSCACNCQRSLFPSMEITELEFVQIVVIVVVMMVMVVMITCLLSHYKLSARSFIS
RHSQARRRDDGLSSEGCLWPSESTVSGGMPEPQVYAPPRPTDRLAVPPFIQRSRFQPTYPYLQHEIALPP
TISLSDGEEPPPYQGPCTLQLRDPEQQLELNRESVRAPPNRTIFDSDLIDSTMLGGPCPPSSNSGISATC
YSSGGRMEGPPPTYSEVIGHYPGSSFQHQQSNGPSSLLEGTRLHHSHIAPLENKEKEKQKGHPL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022995
ORF Size 822 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022995.3, NP_075371.2
RefSeq Size 4531 bp
RefSeq ORF 825 bp
Locus ID 65112
Cytogenetics 2 H3
MW 30.4 kDa
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Documents
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"NM_022995" in other vectors (4)
SKU Description Size Price
MC210236 Pmepa1 (untagged) - Mouse prostate transmembrane protein, androgen induced 1 (Pmepa1), (10ug) 10 ug
$330.00
MG217531 Pmepa1 (tGFP-tagged) - Mouse prostate transmembrane protein androgen induced 1 (Pmepa1), (10ug) 10 ug
$500.00
MR217531L3 Lenti ORF clone of Pmepa1 (Myc-DDK-tagged) - Mouse prostate transmembrane protein, androgen induced 1 (Pmepa1) 10 ug
$600.00
MR217531L4 Lenti ORF clone of Pmepa1 (mGFP-tagged) - Mouse prostate transmembrane protein, androgen induced 1 (Pmepa1) 10 ug
$600.00

Plasmid Map for MR217531

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217531
Circular map for MR217531

file-alt Citations

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