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Rapgefl1 (NM_001080925) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217473
Rapgefl1 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF)-like 1 (Rapgefl1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $616.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rapgefl1
Synonyms B230105J10
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217473 representing NM_001080925
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGCCACTGGAGAAATTTTTGAAGAAGCAGACGTCTCAGCTGGCGGGGCGAGCGGTGACTGGAGGTC
CCGGCGGGGGTCCGGGTTGCTGCGGAGGGCCTGGAGGGGGTGGGGGCCCTGGTGGGGGCGGCGGTCCGGC
CGGGGGACTGCGGCCACTGCAGCGGCGTCAGAGCGTGTCTCGCCTGCTGCTCCCAGCTTTCCTCCGAGAG
CCCCCAACCGAGCCCGGGCTGGAGCCGCCCGTTGAGGAGGAAGGGGGAGAGCCATTAGGGGTCTCTGAGG
AGCCGGGCAGTGGGGGGCCCTGTTGGCTGCAGCTAGAGGAGGTTCCCGGGCCTGGGCCGATAGGATCTGG
GGTGCCCCTGCGCTCTCCTTCCTCGTACTCCTCAGATGAGCTATCCCCCGGCGAACCCCTGGCTTCACCA
CCCTGGGCCCCCCTGGGCGCCCCCGAGCGGCCAGAGCATCTTCTGAACCGGGTTCTGGAGCGCCTGGCTG
GAGGGACCACCAGGGACAGCAGCGCCTCAGATATTCTTCTTGACGACATTGTTCTTACCCATTCCCTCTT
CCTGCCAACAGAAAAGTTTCTACAAGAGCTACACCAGTACTTTGTTCAGTCAAGGAATGTGGAGGGTCCA
GAGGGGCTGGGTCGGAAGCAAGCCTGCCTTGCTCTCCTCCTCCATTTCCTGGATACCTACCAAGGACTTC
TGCAAGAGGAAGAAGGGGCCGGCCACATTATCAAGGAACTGTATCTACTAATTATGAAGGATGAGTCTCT
TTACCAGGACCTCCGAGAGGACACACTGAGGCTACACCAGCTGGTGGAGACAGTGGAGCTAAAGATTCCA
GAAGAGAGCCAGCCGCCCAGCAAGCAGGTTAAGCCGCTCTTCCGACACTTCCGCCGGATAGATTCCTGTC
TGCAGACCCGAGTGGCATTCAGGGGCTCCGATGAGATTTTCTGCCGAGTCTACATGCCTGACCACTCTTA
CGTGACCATACGCAGTCGCCTCTCGGCATCAGTGCAAGACATTCTGGGCTCTGTGACAGAGAAGCTGCAG
TACTCGGAGGAGCCTGCGGAGCGCGAGGATGCCCTCATCCTTGTAGCTGTTGCTTCCTCTGGAGAGAAGG
TCCTCCTCCAGCCAACGGAGGACTGCGTTTTCACCACGCTGGGTATCAACAGCCACCTATTTGCCTGTAC
CCGGGACAGCTACGAGGCCCTAGTACCCCTTCCGGAGGAGGTCCAGGTCTCCCCAGGAGACACAGAGATC
CACCGGGTGGAGCCTGAAGATGTGGCCAATCACCTGACTGCTTTCCACTGGGAGCTCTTCCGATGTGTGC
ACGAGTTGGAGTTCGTGGACTACGTATTCCACGGGGAGCGTGGCCGCCGAGAGACAGCCAACCTGGAGTT
GCTGCTGCAGCGTTGCAGCGAGGTCACTCACTGGGTGGCCACTGAGGTGCTGCTCTGTGAGGCCCCGGGC
AAGCGTGTACAGCTGCTTAAGAAGTTCATCAAGATCGCAGCCATCTGCAAGCAGAACCAGGACCTGCTGT
CCTTCTATGCTGTGGTCATGGGGCTGGACAATGCGGCTGTCAGCCGCCTGCGGCTCACCTGGGAGAAGCT
GCCAGGGAAATTCAAGAACCTGTTCCGTAAATTTGAGGGCTTGACGGACCCCTGCCGGAACCATAAAAGC
TACAGAGAAGTCATCTCCAAAATGAAGCCCCCTCTGATTCCGTTCGTGCCCCTGATCCTCAAAGACCTGA
CCTTCCTACATGAGGGGAGCAAGACCCTCGTAGATGGTCTGGTGAATATTGAGAAGCTGCATTCAGTGGC
CGAGAAAGTGAGGACAATCCGAAAATACCGGAGCCGGCCCCTCTGCCTGGACATGGAGGCATCCCCACAT
CACCTGCAGACCAAGGCGTACGTGCGCCAGTTCCAGGTCATCGACAACCAGAACCACCTCTTTGAGCTCT
CCTACAAGTTGGAGGCTAATAGTCAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217473 representing NM_001080925
Red=Cloning site Green=Tags(s)

MKPLEKFLKKQTSQLAGRAVTGGPGGGPGCCGGPGGGGGPGGGGGPAGGLRPLQRRQSVSRLLLPAFLRE
PPTEPGLEPPVEEEGGEPLGVSEEPGSGGPCWLQLEEVPGPGPIGSGVPLRSPSSYSSDELSPGEPLASP
PWAPLGAPERPEHLLNRVLERLAGGTTRDSSASDILLDDIVLTHSLFLPTEKFLQELHQYFVQSRNVEGP
EGLGRKQACLALLLHFLDTYQGLLQEEEGAGHIIKELYLLIMKDESLYQDLREDTLRLHQLVETVELKIP
EESQPPSKQVKPLFRHFRRIDSCLQTRVAFRGSDEIFCRVYMPDHSYVTIRSRLSASVQDILGSVTEKLQ
YSEEPAEREDALILVAVASSGEKVLLQPTEDCVFTTLGINSHLFACTRDSYEALVPLPEEVQVSPGDTEI
HRVEPEDVANHLTAFHWELFRCVHELEFVDYVFHGERGRRETANLELLLQRCSEVTHWVATEVLLCEAPG
KRVQLLKKFIKIAAICKQNQDLLSFYAVVMGLDNAAVSRLRLTWEKLPGKFKNLFRKFEGLTDPCRNHKS
YREVISKMKPPLIPFVPLILKDLTFLHEGSKTLVDGLVNIEKLHSVAEKVRTIRKYRSRPLCLDMEASPH
HLQTKAYVRQFQVIDNQNHLFELSYKLEANSQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080925
ORF Size 1986 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080925.1, NP_001074394.1
RefSeq Size 4163 bp
RefSeq ORF 1989 bp
Locus ID 268480
UniProt ID Q68EF8
Cytogenetics 11 D
MW 73.7 kDa
Summary Probable guanine nucleotide exchange factor (GEF).UniProtKB/Swiss-Prot Function
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Documents
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"NM_001080925" in other vectors (4)
SKU Description Size Price
MC220289 Rapgefl1 (untagged) - Mouse Rap guanine nucleotide exchange factor (GEF)-like 1 (Rapgefl1), (10ug) 10 ug
$679.00
MG217473 Rapgefl1 (tGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF)-like 1 (Rapgefl1), (10ug) 10 ug
$816.00
MR217473L3 Lenti ORF clone of Rapgefl1 (Myc-DDK-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF)-like 1 (Rapgefl1) 10 ug
$916.00
MR217473L4 Lenti ORF clone of Rapgefl1 (mGFP-tagged) - Mouse Rap guanine nucleotide exchange factor (GEF)-like 1 (Rapgefl1) 10 ug
$916.00

Plasmid Map for MR217473

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217473
Circular map for MR217473

file-alt Citations

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