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Ptcra (NM_011195) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217468
Ptcra (Myc-DDK-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Ptcra or NM_011195"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ptcra
Synonyms gp33; pT-alpha; pT-alpha-TCR; pTa; pTalpha; pTa
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217468 representing NM_011195
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCTCCACGAGTGGGCCATGGCTAGGACATGGCTGCTGCTGCTTCTGGGCGTCAGGTGTCAGGCTC
TACCATCAGGCATCGCTGGCACCCCCTTTCCGTCTCTGGCTCCACCCATCACACTGCTGGTAGATGGAAG
GCAGCACATGCTGGTGGTTTGCCTGGTCCTCGATGCGGCACCCCCTGGCCTTGACAACCCTGTCTGGTTC
TCAGCTGGCAATGGCAGTGCCCTAGACGCCTTCACCTATGGGCCCTCTCTGGCACCGGACGGCACCTGGA
CTAGCCTGGCCCAGCTCTCCTTGCCTTCTGAAGAGCTGGAAGCCTGGGAGCCCTTGGTCTGTCACACCAG
GCCTGGGGCTGGGGGACAGAACCGGAGCACACACCCCCTCCAGCTGTCAGGGGAATCTTCGACAGCCAGG
AGCTGCTTTCCGGAGCCTCTCGGGGGGACACAGCGGCAGGTACTGTGGCTGAGCCTACTGCGCCTGCTCC
TCTTCAAACTGCTTCTGCTCGATGTGCTCCTGACCTGCAGCCACCTCCGCCTCCATGTGCTGGCGGGCCA
GCACCTCCAGCCACCACCCTCACGCAAGTCCCTGCCTCCCACCCACAGAATTTGGACA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217468 representing NM_011195
Red=Cloning site Green=Tags(s)

MLLHEWAMARTWLLLLLGVRCQALPSGIAGTPFPSLAPPITLLVDGRQHMLVVCLVLDAAPPGLDNPVWF
SAGNGSALDAFTYGPSLAPDGTWTSLAQLSLPSEELEAWEPLVCHTRPGAGGQNRSTHPLQLSGESSTAR
SCFPEPLGGTQRQVLWLSLLRLLLFKLLLLDVLLTCSHLRLHVLAGQHLQPPPSRKSLPPTHRIWT

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_011195
ORF Size 618 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_011195.2, NP_035325.1
RefSeq Size 1283 bp
RefSeq ORF 621 bp
Locus ID 19208
UniProt ID P0C6B2
Cytogenetics 17 C
MW 22.8 kDa
Summary The pre-T-cell receptor complex (composed of PTCRA, TCRB and the CD3 complex) regulates early T-cell development. Isoform 1 acts to retain most TCRB intracellularly, while isoform 2 permits higher levels of cell surface TCRB expression and facilitates signaling from the CD3-TCRB complex.UniProtKB/Swiss-Prot Function
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"NM_011195" in other vectors (4)
SKU Description Size Price
MC209251 Ptcra (untagged) - Mouse pre T-cell antigen receptor alpha (Ptcra), (10ug) 10 ug
$330.00
MG217468 Ptcra (tGFP-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra), (10ug) 10 ug
$500.00
MR217468L3 Lenti ORF clone of Ptcra (Myc-DDK-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra) 10 ug
$600.00
MR217468L4 Lenti ORF clone of Ptcra (mGFP-tagged) - Mouse pre T-cell antigen receptor alpha (Ptcra) 10 ug
$600.00

Plasmid Map for MR217468

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217468
Circular map for MR217468

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