Rpusd3 (NM_001033204) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR217467
Rpusd3 (Myc-DDK-tagged) - Mouse RNA pseudouridylate synthase domain containing 3 (Rpusd3)
$289.00 $457.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rpusd3
Synonyms AI527266
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217467 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAGCCCTGAGAGTTTTGCGTTATGTCTCTATGATCTGGCGGCCGGAGCTGGGGTCTTGCGCACGGC
AGAGGGATGCCGGCTTCGGCACCGAGGCCCGGCGTCCGTCTCAACCTCACCGCTCCAGCAAGCACAAGGA
CCTAGTCGAGGACCAGCCCTTCCCGGGGCTGCTGAGGACGGAGAACCTCGGCCTGGAGGAGCTGGCTCAT
GTCCTGAGGGCAGCTGTGGTGGATCAGAAAGGACCTCTGGTGACACTGAACAAGCCACAGGGTCTGCCTG
TGACAGGGAGACCAGGAGAGCTGACGCTGCTATCTGTGCTGCCGCAGCTGAGCCAGGCCCTGGGGCTCGA
ACACCAGGAGCTCCAGGTGGTGCGAGCACCTGGGAAGGAGGCCTCCGGACTTGTGCTCCTCTCCAGCTGT
CCCCAGACAGCAAGCCGCCTGCAGAAGTTCTTTATCCACTCAAGGAGAGCACAGAGACCCACAGCCACCT
ACTGTGCTGTCACTGATGGCATCCCTGAGCCTTCAGAGGGGACAGTCTGCATGCCTCTGAAGATGGAACA
AATGAATGACGTTGATCTTGCAGTCCCAGTGATGTCCCCATCTCGAAAGGACATCCAGGAAGGTGTCAAA
AGGACCCTCAGCCGTTTCCATGTGATGGCCACAGGCCGTGGCTGTGCCCTGGTCCAGCTGCAGCCACTGA
CAGTCTTCCCCAATCAGCTGCAGGTACACATGGCTCTACAGCTCTGTCCAATTCTTGGGGACCACACTTA
TGCTGCTCGTGTGGGCACCGTTCTGGGCCAGCGCTTTCTGTGGCCAGCCGAGACCACCAAGCCCCAGAGA
CAGGTCCTGGATGAAGCCCTGCTCAGACACCTCCGCCTGAGTCCTTCCCAAGTGGCCCAGATGCCTTTGC
ACCTGCACCTTCACCGCCTCCTCCTCCCAGGCACCGGATCCAGGGACCCCCCTAGTGAACTTCTGGCACC
CCTGCCCCCTTACTTCTCTCGGACCCTGCAGTGCCTTCGGCTCTCTCAACAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR217467 protein sequence
Red=Cloning site Green=Tags(s)

MGALRVLRYVSMIWRPELGSCARQRDAGFGTEARRPSQPHRSSKHKDLVEDQPFPGLLRTENLGLEELAH
VLRAAVVDQKGPLVTLNKPQGLPVTGRPGELTLLSVLPQLSQALGLEHQELQVVRAPGKEASGLVLLSSC
PQTASRLQKFFIHSRRAQRPTATYCAVTDGIPEPSEGTVCMPLKMEQMNDVDLAVPVMSPSRKDIQEGVK
RTLSRFHVMATGRGCALVQLQPLTVFPNQLQVHMALQLCPILGDHTYAARVGTVLGQRFLWPAETTKPQR
QVLDEALLRHLRLSPSQVAQMPLHLHLHRLLLPGTGSRDPPSELLAPLPPYFSRTLQCLRLSQQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001033204
ORF Size 1032 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033204.1, NP_001028376.1
RefSeq Size 1269 bp
RefSeq ORF 1035 bp
Locus ID 101122
UniProt ID Q14AI6
Cytogenetics 6 E3
MW 38.1 kDa
Summary Catalyzes uridine to pseudouridine isomerization (pseudouridylation) of specific mitochondrial mRNAs (mt-mRNAs), a post-transcriptional modification necessary for their translation. Acts at position 390 in COXI mt-mRNA and at position 697-699 in mitochondrial COXIII mt-mRNA. As a component of a functional protein-RNA module, consisting of RCC1L, NGRN, RPUSD3, RPUSD4, TRUB2, FASTKD2 and 16S mitochondrial ribosomal RNA (16S mt-rRNA), controls 16S mt-rRNA abundance and may play a role in mitochondrial ribosome biogenesis.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033204" in other vectors (4)
SKU Description Size Price
MC212067 Rpusd3 (untagged) - Mouse RNA pseudouridylate synthase domain containing 3 (Rpusd3), (10ug) 10 ug
$503.00
MG217467 Rpusd3 (tGFP-tagged) - Mouse RNA pseudouridylate synthase domain containing 3 (Rpusd3), (10ug) 10 ug
$657.00
MR217467L3 Lenti ORF clone of Rpusd3 (Myc-DDK-tagged) - Mouse RNA pseudouridylate synthase domain containing 3 (Rpusd3) 10 ug
$757.00
MR217467L4 Lenti ORF clone of Rpusd3 (mGFP-tagged) - Mouse RNA pseudouridylate synthase domain containing 3 (Rpusd3) 10 ug
$757.00

Plasmid Map for MR217467

Download sequence for

Cloning scheme for MR217467

Circular map for MR217467

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