OriGene Logo

Customer Login

Checkout as a new customer

Creating an account has many benefits:

  • See order and shipping status
  • Track order history
  • Check out faster
Create an Account

Oaz3 (NM_016901) Mouse Tagged ORF Clone

  • TrueORF®
Be the first to review this product
SKU
MR217445
Oaz3 (Myc-DDK-tagged) - Mouse ornithine decarboxylase antizyme 3 (Oaz3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (5)

TA592569S DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
30 ul
$200.00
CC100003 DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
5 x 200 ul
$170.00
PRIMER Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
100 pmol
$59.00
PS100001 pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
10 ug
$670.00
AR100028M DDKTrap Anti-DDK 8G6 affinity resin used for the purification of DDK, DYKDDDDK, or FLAG® tagged proteins , 5 mL
5 ml
$750.00

Other products for "Oaz3 or NM_016901"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Oaz3
Synonyms AZ; AZ-; Az-3; AZ3; Oaz; Oaz-t
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217445 representing NM_016901
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

CTGCCTTGTAACAGGTCCCGCCCCTCTCTCTACTCCCTTTCTTATATCAAGAGGGGAAAAACACGGAACT
ATCTCTATCCATTCTGGTCACCATTCGCCTATTACCTCTACTGTTACAAATACCGGATCACCCTCCGGGA
GAAGATGCTGCCTTGTTGTTACAAAAGCATCACTTACAAGGAACAGGAGGACCTGACTCTCCGGCCCCAT
TGCTGCCTCCCGTGCTCCTGCCTCCCGTGCTCCTGCCTCCAGTGCTCCCTGCCTTGTAACAGGTCCCGCC
CCTCTCTCTACTCCCTTTCTTATATCAAGAGGGGAAAAACACGGAACTATCTCTATCCATTCTGGTCACC
ATTCGCCTATTACCTCTACTGTTACAAATACCGGATCACCCTCCGGGAGAAGATGCTGCCTTGTTGTTAC
AAAAGCATCACTTACAAGGAACAGGAGGACCTGACTCTCCGGCCCCATTGCTGCCTCCCGTGCTCCTGCC
TCCCGTGCTCCTGCCTCCAGTGCTCC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217445 representing NM_016901
Red=Cloning site Green=Tags(s)

LPCNRSRPSLYSLSYIKRGKTRNYLYPFWSPFAYYLYCYKYRITLREKMLPCCYKSITYKEQEDLTLRPH
CCLPCSCLPCSCLQCSLPCNRSRPSLYSLSYIKRGKTRNYLYPFWSPFAYYLYCYKYRITLREKMLPCCY
KSITYKEQEDLTLRPHCCLPCSCLPCSCLQCS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016901
ORF Size 516 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016901.3, NP_058597.2
RefSeq Size 955 bp
RefSeq ORF 733 bp
Locus ID 53814
Cytogenetics 3 F2.1
MW 20.7 kDa
Summary The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 3, the third member of the antizyme family. Like antizymes 1 and 2, antizyme 3 inhibits ODC activity and polyamine uptake; however, it does not stimulate ODC degradation. Also, while antizymes 1 and 2 have broad tissue distribution, expression of antizyme 3 is restricted to haploid germ cells in testis, suggesting a distinct role for this antizyme in spermiogenesis. Antizyme 3 gene knockout studies showed that homozygous mutant male mice were infertile, and indicated the likely role of this antizyme in the formation of a rigid connection between the sperm head and tail during spermatogenesis. This transcript initiates translation from a non-AUG (CUG) codon that is highly conserved among the antizyme 3 orthologs. provided by RefSeq, Dec 2014
Reviews
Write Review
 
 
 
 
 

Be the first to review this product

Please note:
Only reviews with images are eligible for a $20/20€ Amazon gift card.
Reviews without images are eligible for a $10/10€ Amazon gift card.
For more details about the terms and conditions, please visit the promotion page.

Review image
Documents
Resources
"NM_016901" in other vectors (4)
SKU Description Size Price
MC209944 Oaz3 (untagged) - Mouse ornithine decarboxylase antizyme 3 (Oaz3), (10ug) 10 ug
$330.00
MG217445 Oaz3 (tGFP-tagged) - Mouse ornithine decarboxylase antizyme 3 (Oaz3), (10ug) 10 ug
$500.00
MR217445L3 Lenti ORF clone of Oaz3 (Myc-DDK-tagged) - Mouse ornithine decarboxylase antizyme 3 (Oaz3) 10 ug
$600.00
MR217445L4 Lenti ORF clone of Oaz3 (mGFP-tagged) - Mouse ornithine decarboxylase antizyme 3 (Oaz3) 10 ug
$600.00

Plasmid Map for MR217445

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217445
Circular map for MR217445

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Related Products