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Cog3 (NM_177381) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217257
Cog3 (Myc-DDK-tagged) - Mouse component of oligomeric golgi complex 3 (Cog3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $758.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cog3
Synonyms E430004N23
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217257 representing NM_177381
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGAGGCGGCGCTGCTGCTGCCGCCCGAGGCGGCGGCCGAGCGGGACGCCCGCGAGAAGCTGTCTC
TCTGGGATGGGAGACCCGACTCGATGGCGCCGCTGACAGACAGGCAGACGGACTCGGTGCTGGAGCTCAA
GGCGGCCGTGGAGAACCTGCCGGTGCCCGCCGAGCTCCCAATTGAAGATGTATGCAGCTTAGCATCCCAG
TCGCTGCCCATTGAGCTGACTGCTGTAGTGCCTGACTCTACAGAAGACATCCTGTTAAAGGGCTTCACTT
CCTTAGGAATGGAAGAAGAAAGGATTGAGACTGCGCAGCAGTTTTTCTCATGGTTTGCAAAACTGCAAAC
TCAGATGGATCAGGATGAAGGAACTAAATATAGACAGATGAGGGATTACTTGTCTGGGTTTCAGGAACAG
TGTGATGCTATACTGAATGACGTGAACAGTGCTCTGCAGCATCAGGAGTCGTTGCAGAAACAGTATCTTT
TTGTGTCCAATAAGACAGGAACTCTTCATGAAGCCTGTGAACAGCTCCTAAAAGAACAGTCGGAGCTTGC
TGATCTTGCTGAGCACATCCAACAGAAGCTGTCTTATTTTAATGAGTTGGAGACGATTAACACAAAATTG
AATTCCCCTACGTTGTCTGTGAATAGTGAAGGATTTATCCCTATGTTGGCCAAGTTAGATGATTGTATAA
CATATATTTCATCGCATCCTAATTTTAAAGATTATCCTGTATACTTACTGAAGTTTAAACAATGTCTTTC
TAAAGCTTTGCACCTCATGAAGACATATACTGTGAACACACTGCAGACTCTCACAAATCAGTTACTAAAA
AGGGACCCCTCATCAGTCCCTAATGCAGACAATGCCTTCACACTATTTTATGTGAAATTTCGAGCCGCTG
CCCCTAAAGTCAGAACTCTAATTGAACAGATAGAGCAACGATCTGAAAAGATACCCGAATACCAGCACCT
TCTCAATGACATCCACCAGTGTTACCTTGATCAGCGGGAGCTCCTTTTGGGCCCAAGTATTGCCTACACT
GTCACAGAGTTAACCAGCCAGAACAACAGAGACCATTGTGCCTTGGTTCGCAGTGGCTGTGCCTTTATGG
TCCATGTCTGCCAAGATGAGCACCAACTTTACAATGAATTTTTCACAAAGCCAACATCAAAATTAGATGA
GCTATTGGAGAAATTGTGCGTGTCGTTGTATGATGTGTTCCGGCCCTTGATCATCCATGTCATTCACTTA
GAGACTTTATCGGAGCTCTGTGGGATTCTTAAAAACGAAGTGCTTGAAGACCACGTACAGCACAACGCTG
AACAACTGGGGGCATTTGCAGCTGGAGTAAAGCAGATGTTGGAAGATGTACAAGAGAGGCTTGTGTATCG
AACCCATATCTACATTCAGACGGACATCACAGGCTACAAACCAGCGCCTGGAGATCTGGCATATCCTGAT
AAGCTAGTCATGATGGAGCAAATCGCACAGAGTTTAAAGGATGAGCAGAAGAAGGCGCCTTCAGAAGCTT
CCTTTTCAGATGTACGGTTAGAAGAAGGAGAAGCCAGTGGTTTAAGAAAGTCTGGTTCAACAGACTCCCT
CAATCCTAGACCACAGACGACGATCTCTCCAGCAGATCTTCACGGGATGTGGTATCCCACAGTTCGAAGG
ACACTTGTCTGTCTCTCCAAATTATATAGATGCATAGATAGGGCGGTGTTCCAAGGATTATCACAGGAAG
CCTTGTCTGCCTGCATACAGTCCTTGCTAGGAGCATCAGAGTCTATCAGCAAAAACAAGACTCAGATTGA
TGGACAACTTTTCTTAATTAAACACCTTTTGATTCTTCGAGAACAAATTGCTCCATTTCACACTGAATTC
ACCATTAAGGAAATTTCCCTGGACCTCAAGAAAACTAGAGATGCAGCATTTAAAATCCTGAACCCTATGA
CTGTTCCACGATTTTTTAGGCTGAATAGCAACAATGCCTTGATAGAGTTCTTGTTGGAGGGGACTCCTGA
GATCAGAGAACATTATCTTGACTCTAAGAAGGACGTAGACCGGCACCTGAAGTCAGCCTGTGAGCAGTTC
ATTCAGCAGCAGACCAGGCTGTTTGTAGAGCAGCTGGAGGAGTTCATGACAAAGGTATCAGCATTGAAAA
CCATGGCCAGCCAGGGAGGCCCCAAGTACACCCTCTCACAGCAGCCGTGGGCACAGCCAGCAAAGGTCAA
TGACCTCGTGGCCACTGCCTACAAGACAATAAAGACGAAGCTGCCGCTGACACTGAGAAGCATGGCTTTG
TATTTGTCCAATAAGGACACGGAGTTCATCTTGTTTAAGCCTGTCAGGAATAACATTCAGCAAGTCTTCC
AGAAGTTCCACGCTCTGCTAAAGGAAGAGTTCAGTTCTGAAGACATCCAAATCATTGCTTGTCCTTCCAT
GGAACAGCTGAACCTCCTGCTGTCAGTTTCAAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217257 representing NM_177381
Red=Cloning site Green=Tags(s)

MAEAALLLPPEAAAERDAREKLSLWDGRPDSMAPLTDRQTDSVLELKAAVENLPVPAELPIEDVCSLASQ
SLPIELTAVVPDSTEDILLKGFTSLGMEEERIETAQQFFSWFAKLQTQMDQDEGTKYRQMRDYLSGFQEQ
CDAILNDVNSALQHQESLQKQYLFVSNKTGTLHEACEQLLKEQSELADLAEHIQQKLSYFNELETINTKL
NSPTLSVNSEGFIPMLAKLDDCITYISSHPNFKDYPVYLLKFKQCLSKALHLMKTYTVNTLQTLTNQLLK
RDPSSVPNADNAFTLFYVKFRAAAPKVRTLIEQIEQRSEKIPEYQHLLNDIHQCYLDQRELLLGPSIAYT
VTELTSQNNRDHCALVRSGCAFMVHVCQDEHQLYNEFFTKPTSKLDELLEKLCVSLYDVFRPLIIHVIHL
ETLSELCGILKNEVLEDHVQHNAEQLGAFAAGVKQMLEDVQERLVYRTHIYIQTDITGYKPAPGDLAYPD
KLVMMEQIAQSLKDEQKKAPSEASFSDVRLEEGEASGLRKSGSTDSLNPRPQTTISPADLHGMWYPTVRR
TLVCLSKLYRCIDRAVFQGLSQEALSACIQSLLGASESISKNKTQIDGQLFLIKHLLILREQIAPFHTEF
TIKEISLDLKKTRDAAFKILNPMTVPRFFRLNSNNALIEFLLEGTPEIREHYLDSKKDVDRHLKSACEQF
IQQQTRLFVEQLEEFMTKVSALKTMASQGGPKYTLSQQPWAQPAKVNDLVATAYKTIKTKLPLTLRSMAL
YLSNKDTEFILFKPVRNNIQQVFQKFHALLKEEFSSEDIQIIACPSMEQLNLLLSVSK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_177381
ORF Size 2484 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_177381.3, NP_796355.2
RefSeq Size 4222 bp
RefSeq ORF 2487 bp
Locus ID 338337
Cytogenetics 14 D3
MW 94.1 kDa
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Documents
Resources
"NM_177381" in other vectors (4)
SKU Description Size Price
MC221914 Cog3 (untagged) - Mouse component of oligomeric golgi complex 3 (Cog3), (10ug) 10 ug
$835.00
MG217257 Cog3 (tGFP-tagged) - Mouse component of oligomeric golgi complex 3 (Cog3), (10ug) 10 ug
$958.00
MR217257L3 Lenti ORF clone of Cog3 (Myc-DDK-tagged) - Mouse component of oligomeric golgi complex 3 (Cog3) 10 ug
$1,058.00
MR217257L4 Lenti ORF clone of Cog3 (mGFP-tagged) - Mouse component of oligomeric golgi complex 3 (Cog3) 10 ug
$1,058.00

Plasmid Map for MR217257

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217257
Circular map for MR217257

file-alt Citations

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