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Ano4 (NM_178773) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217086
Ano4 (Myc-DDK-tagged) - Mouse anoctamin 4 (Ano4)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $718.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Ano4
Synonyms A330096O15Rik; Gm65; Tmem16d
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217086 representing NM_178773
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGTTTTCTTCTCGACTGGCTTCATTTTTGCTGTTCTTCCAGGAGAAAAATCTATTACCTGCCCCGCC
GGTACAAGTTCATGAGCAGGATCGATAAACAAATAAGCAGGTTCCGGAGATGGTTACCTAAGAAGCCAAT
GAGGCTGGACAAGGAGACACTGCCAGACCTGGAAGAGAATGACTGCTACACTGCCCCTTTCAGCCAACAA
AGGATCCATCACTTCATCATACACAACAAAGACACTTTCTTCAACAATGCCACAAGAAGCAGAATCGTGC
ATCATATTTTACAAAGAATAAAGTACGAGGAAGGAAAGAATAAAATCGGTCTGAACCGCTTGCTTACCAA
TGGCTCCTATGAAGCTGCCTTTCCCCTGCATGAGGGAAGTTACAGGAGTAAAAACTCCATTAAAACCCAT
GGAGCTGTAAACCACCGACATCTACTGTATGAGTGCTGGGCCTCCTGGGGCGTGTGGTACAAGTACCAGC
CTTTGGATCTTGTCAGGCGATATTTTGGAGAGAAGATTGGACTGTATTTCGCCTGGCTGGGCTGGTACAC
AGGCATGCTCTTCCCCGCAGCCTTCATTGGGTTGTTTGTCTTCTTGTATGGAGTCACCACTCTGGATCAC
TGCCAAGTCAGTAAAGAAGTCTGCCAAGCAACAGATATCATCATGTGTCCTGTATGTGACAAATACTGTC
CATTCATGAGGCTTTCAGACAGCTGTGTCTATGCCAAGGTGACCCACCTTTTTGACAATGGGGCCACTGT
GTTCTTTGCTGTGTTCATGGCAGTCTGGGCTACTGTTTTCCTGGAATTCTGGAAGAGACGCCGAGCAGTG
ATCGCTTATGACTGGGATCTGATTGACTGGGAAGAAGAGGAGGAAGAAATACGACCCCAGTTTGAAGCCA
AGTATTCCAAGAAAGAGCGGATGAATCCCATTTCAGGAAAGCCAGAACCTTATCAAGCATTTACTGATAA
ATGCAGCAGACTTATCGTTTCTGCATCAGGAATATTCTTCATGATCTGCGTGGTGATCGCTGCTGTGTTC
GGGATCGTCATTTACAGGGTGGTGACTGTGAGCACTTTCGCTGCCTTTAAGTGGGCATTAATCAGGAATA
ACTCTCAGGTTGCAACCACAGGGACTGCAGTATGCATCAACTTTTGTATCATTATGCTGCTGAATGTGCT
CTATGAAAAAGTCGCACTCCTTCTAACAAATCTGGAACAGCCTCGCACAGAATCCGAGTGGGAGAACAGC
TTTACCTTGAAAATGTTTCTTTTTCAGTTTGTCAATCTGAACAGCTCCACTTTTTACATCGCATTCTTCC
TCGGAAGATTTACAGGACACCCAGGTGCCTACTTGAGATTGATAAACAGGTGGAGGCTAGAAGAGTGCCA
CCCTAGCGGATGCCTTATTGATCTCTGCATGCAAATGGGTATTATAATGGTGCTAAAGCAGACCTGGAAT
AATTTCATGGAACTTGGCTACCCGTTAATTCAAAATTGGTGGACTAGAAGAAAAGTCCGACAAGAACATG
GGACTGAAAGGAAAATAAATTTCCCACAGTGGGAAAAGGACTACAATCTTCAGCCGATGAATGCCTATGG
ACTCTTCGATGAATACCTGGAAATGATCCTTCAGTTCGGATTCACAACGATCTTTGTGGCAGCTTTTCCT
CTAGCACCGCTTCTGGCCTTACTGAATAACATCATTGAAATCCGACTTGATGCTTATAAGTTTGTTACTC
AGTGGAGAAGGCCTTTGGCTTCGAGAGCCAAAGACATAGGAATTTGGTATGGAATTCTCGAAGGCATTGG
GATTCTGTCTGTTATTACAAATGCATTTGTCATAGCAATAACATCCGACTTCATCCCTCGCTTAGTATAT
GCTTATAAATATGGACCTTGTGCAGGCCAAGGAGAAGCTGGGCAAAAGTGCATGGTTGGCTATGTGAATG
CCAGCTTGTCTGTGTTCCGAATTTCTGACTTTGAAAATCGGTCGGAGCCAGAGTCTGATGGCAGTGAGTT
CTCAGGAACCCCTCTGAAGTACTGCAGATATCGAGACTACCGGGACCCTCCTCACTCTTTGGCACCCTAT
GGCTACACACTGCAGTTCTGGCATGTTCTCGCAGCTCGGCTGGCTTTTATCATTGTGTTTGAGCACCTCG
TGTTTTGTATAAAGCACCTCATTTCCTATCTGATACCAGACCTCCCGAAAGATCTAAGGGACCGGATGAG
GAGAGAGAAGTACTTGATCCAGGAGATGATGTATGAGGCTGAGCTGGAGCGCCTCCAGAAAGAACGGAAG
GAGAGGAAGAAGAACGGAAAAGCACATCACAATGAGTGGCCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217086 representing NM_178773
Red=Cloning site Green=Tags(s)

MGFLLDWLHFCCSSRRKIYYLPRRYKFMSRIDKQISRFRRWLPKKPMRLDKETLPDLEENDCYTAPFSQQ
RIHHFIIHNKDTFFNNATRSRIVHHILQRIKYEEGKNKIGLNRLLTNGSYEAAFPLHEGSYRSKNSIKTH
GAVNHRHLLYECWASWGVWYKYQPLDLVRRYFGEKIGLYFAWLGWYTGMLFPAAFIGLFVFLYGVTTLDH
CQVSKEVCQATDIIMCPVCDKYCPFMRLSDSCVYAKVTHLFDNGATVFFAVFMAVWATVFLEFWKRRRAV
IAYDWDLIDWEEEEEEIRPQFEAKYSKKERMNPISGKPEPYQAFTDKCSRLIVSASGIFFMICVVIAAVF
GIVIYRVVTVSTFAAFKWALIRNNSQVATTGTAVCINFCIIMLLNVLYEKVALLLTNLEQPRTESEWENS
FTLKMFLFQFVNLNSSTFYIAFFLGRFTGHPGAYLRLINRWRLEECHPSGCLIDLCMQMGIIMVLKQTWN
NFMELGYPLIQNWWTRRKVRQEHGTERKINFPQWEKDYNLQPMNAYGLFDEYLEMILQFGFTTIFVAAFP
LAPLLALLNNIIEIRLDAYKFVTQWRRPLASRAKDIGIWYGILEGIGILSVITNAFVIAITSDFIPRLVY
AYKYGPCAGQGEAGQKCMVGYVNASLSVFRISDFENRSEPESDGSEFSGTPLKYCRYRDYRDPPHSLAPY
GYTLQFWHVLAARLAFIIVFEHLVFCIKHLISYLIPDLPKDLRDRMRREKYLIQEMMYEAELERLQKERK
ERKKNGKAHHNEWP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_178773
ORF Size 2352 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_178773.4, NP_848888.3
RefSeq Size 4134 bp
RefSeq ORF 2355 bp
Locus ID 320091
Cytogenetics 10 C1-C2
MW 151.6 kDa
Summary Has calcium-dependent phospholipid scramblase activity; scrambles phosphatidylserine, phosphatidylcholine and galactosylceramide (PubMed:23532839). Does not exhibit calcium-activated chloride channel (CaCC) activity (PubMed:22075693).UniProtKB/Swiss-Prot Function
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"NM_178773" in other vectors (4)
SKU Description Size Price
MC221604 Ano4 (untagged) - Mouse anoctamin 4 (Ano4), (10ug) 10 ug
$791.00
MG217086 Ano4 (tGFP-tagged) - Mouse anoctamin 4 (Ano4), (10ug) 10 ug
$918.00
MR217086L3 Lenti ORF clone of Ano4 (Myc-DDK-tagged) - Mouse anoctamin 4 (Ano4) 10 ug
$1,018.00
MR217086L4 Lenti ORF clone of Ano4 (mGFP-tagged) - Mouse anoctamin 4 (Ano4) 10 ug
$1,018.00

Plasmid Map for MR217086

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217086
Circular map for MR217086

file-alt Citations

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