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Gramd3 (NM_026240) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR217084
Gramd3 (Myc-DDK-tagged) - Mouse GRAM domain containing 3 (Gramd3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Gramd3
Synonyms 9030613F08Rik; 9130427A09Rik; AW061306; NS3TP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR217084 representing NM_026240
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACCGAGCTCCAGCAAGAGGTGGAGGACGCCAAGCCAGCCAAAGTGCTCACCAAGAGAGAAGGCAAAG
TTGGCCCGGCCCACTCAGAGGCTGAGAATGGTGCGGAGGACAGAAGGAGGGTCAGCAAGTCGCCAACAGC
CCAATCTCCTACCTCATCTGTGGAGGCCGAGTCCCCGGACCAGAAGAGGAGCCTTGGCCTGTGGTCAAAA
TCCAGTTTTGATGGTTCCAGTTTGTTGAGTGATAAGAATGACTGTAAAACTGAAAGCAAGACTGACTCTA
AGACCGAAAGAAAAAAGTCTTCATCGTCTAGTCAGTACAAGGCGAACATGCACTTTCACAAGTTATTCCT
GGATGTCCCAACTGAGGAGCCACTGAGGCAAAGCTTTACCTGTGCTCTTCAGAAAGAAATTCTGTACCAA
GGAAAGCTCTTTGTGTCGGAAAACTGGATTTGTTTTCATTCCAAGGTCTTTGGAAAAGACACTAAGATCT
CCATTCCAGCTTTCTCGGTAACCTTGATCAAGAAAACCAAAACTGCCCTTCTGGTGCCAAACGCCCTGAT
CATCGCGACGGTCACAGACAGGTACATATTCGTTTCCTTGCTGTCCAGAGACTCAACCTACAAACTGATA
AAATCTGTGTGCGGACACTTAGAGAACACAAGTGTTGGTAACAGTCCCAACCCATCTTCTGCTGAAAACA
GTTTCCGGGCAGATCGCCCTTCGTCTCTGCCTCTGGATTTCAATGATGAATTCTCAGACCTGGACGGAGT
GGTTCGACAACGAAGGCAAGACTTGGAAGGATACAGCAGCTCAGGATCCCAGACTCCGGAATCTGAGAAC
TCCCGAGACTTCCACGTGACCGAATCTCAGACAGTTCTGAATGTCACAAAGGGAGAAACAAAACCACCTC
GGACAGATGCCCACGGGAGCAGAGCACCTGATGGAAAAGCCAAGATCCTGCCTGCCCATGGTCAATCAGA
AACTATTGGAATTTTACATAAAATGGAGTCTCGGAAGTGCCCGACTCTCCATCACATCCTGATAGTCTAT
GCAATTATTGTCTGTGCCCTGATCATCTCCACCTTCTACATGAGATACAGGATTAACACTCTGGAGGAGC
GCCTGGGGACCCTGACGTCCATCATGGATCCCCACAGTACTGAGCAGACAGCCCCATCCGGCCTGGGATC
ACAAATGCAGTTAAATGTGGAGGTCCTCTGCCAAGAACTTACAGCTAACATAGTGAAATTGGAAAAGATA
CAAAACAACTTACAAAAGCTGCTTGAGAATGGTGAC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR217084 representing NM_026240
Red=Cloning site Green=Tags(s)

MTELQQEVEDAKPAKVLTKREGKVGPAHSEAENGAEDRRRVSKSPTAQSPTSSVEAESPDQKRSLGLWSK
SSFDGSSLLSDKNDCKTESKTDSKTERKKSSSSSQYKANMHFHKLFLDVPTEEPLRQSFTCALQKEILYQ
GKLFVSENWICFHSKVFGKDTKISIPAFSVTLIKKTKTALLVPNALIIATVTDRYIFVSLLSRDSTYKLI
KSVCGHLENTSVGNSPNPSSAENSFRADRPSSLPLDFNDEFSDLDGVVRQRRQDLEGYSSSGSQTPESEN
SRDFHVTESQTVLNVTKGETKPPRTDAHGSRAPDGKAKILPAHGQSETIGILHKMESRKCPTLHHILIVY
AIIVCALIISTFYMRYRINTLEERLGTLTSIMDPHSTEQTAPSGLGSQMQLNVEVLCQELTANIVKLEKI
QNNLQKLLENGD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026240
ORF Size 1296 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026240.2, NP_080516.2
RefSeq Size 2611 bp
RefSeq ORF 1299 bp
Locus ID 107022
Cytogenetics 18 D3
MW 48.4 kDa
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"NM_026240" in other vectors (4)
SKU Description Size Price
MC212136 Gramd3 (untagged) - Mouse GRAM domain containing 3 (Gramd3), (10ug) 10 ug
$503.00
MG217084 Gramd3 (tGFP-tagged) - Mouse GRAM domain containing 3 (Gramd3), (10ug) 10 ug
$657.00
MR217084L3 Lenti ORF clone of Gramd3 (Myc-DDK-tagged) - Mouse GRAM domain containing 3 (Gramd3) 10 ug
$757.00
MR217084L4 Lenti ORF clone of Gramd3 (mGFP-tagged) - Mouse GRAM domain containing 3 (Gramd3) 10 ug
$757.00

Plasmid Map for MR217084

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR217084
Circular map for MR217084

file-alt Citations

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