Dsg1a (NM_010079) Mouse Tagged ORF Clone
$589.00
$920.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Dsg1a |
| Synonyms | DG1; DGI; Dsg; Dsg1; dsg1-alpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_010079 |
| ORF Size | 3171 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_010079.2, NP_034209.2 |
| RefSeq Size | 5627 bp |
| RefSeq ORF | 3174 bp |
| Locus ID | 13510 |
| UniProt ID | Q61495 |
| Cytogenetics | 18 11.29 cM |
| MW | 114.6 kDa |
| Summary | This gene encodes a member of the cadherin family of proteins that forms an integral transmembrane component of desmosomes, the multiprotein complexes involved in cell adhesion, organization of the cytoskeleton, cell sorting and cell signaling. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional protein. This gene is located in a cluster of desmosomal cadherin genes on chromosome 18. provided by RefSeq, Jan 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_010079" in other vectors (4)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC223389 | Dsg1a (untagged) - Mouse desmoglein 1 alpha (Dsg1a), (10ug) | 10 ug |
$1,013.00
|
|
| MG217075 | Dsg1a (tGFP-tagged) - Mouse desmoglein 1 alpha (Dsg1a), (10ug) | 10 ug |
$1,120.00
|
|
| MR217075L3 | Lenti ORF clone of Dsg1a (Myc-DDK-tagged) - Mouse desmoglein 1 alpha (Dsg1a) | 10 ug |
$1,220.00
|
|
| MR217075L4 | Lenti ORF clone of Dsg1a (mGFP-tagged) - Mouse desmoglein 1 alpha (Dsg1a) | 10 ug |
$1,220.00
|
|
Citations
mTORC1 loss impairs epidermal adhesion via TGF-β/Rho kinase activation
Asrani, Sood, Torres et al
J. Clin. Invest. (2017)
Asrani, Sood, Torres et al
J. Clin. Invest. (2017)
Showing 1-1 of 1 papers.
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