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Krtcap3 (NM_027221) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR216892
Krtcap3 (Myc-DDK-tagged) - Mouse keratinocyte associated protein 3 (Krtcap3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Krtcap3
Synonyms 2010001C09Rik; AW321088; Kcp3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR216892 representing NM_027221
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGTGCTGCGGCGTCTGCGCCTTCGATGCCGCCCGAGGGCCCAGGCGGCTGATGCGCGTGGGACTCG
CGCTGATCCTGGTGGGCCACGTGAACCTCCTGGTGGGAGCTGTGTTGCATGGTACTGTTCTGCGGCACGT
GGCCAACCCCCGAGGCGCGGTCACTCCTGAGTACACCACAGCCAATGTCATTTCTGTGGGTTCGGGACTA
CTGAGCGTTTCAGTGGGACTTGTGGCCCTCTTGGCATCCAGGAACCTTCTTCGCCCACGACTGCACTGGG
CCCTGCTGACGCTGGCCTTGGTGAACCTGCTCTTGTCTGCTGCCTGCTCCATGGGCCTCCTCCTGGCTGT
ATCGCTCACTGTTGCCAATGGTGGCCGCCGTCTTATTGCTGATTGCCACCCAGGACTGATGGATCCTTCC
ATACCGCTGGACCAGGGGCCTGGACACACTGACTGCTCCTTTGATCCCACAAGAATTTATGATACAGCTT
TGGCTCTCTGGATCCCTTCTCTGTTCATGTCTGCAGCTGAGGCTGCTCTTTCTGGTTACTGTTGTGTGGC
TGCACTCACGCTACGGGGAATTGGGCCCTGCAGGAAGGAAGGGTTGCAGGAACAGCTGCAGGAACTGACA
GAGCTTGAACTTCCCGAGTGTAAAAGGCAGGAGAACGTGCAGCTGCTCCATGGCCGTCAGGACTTCCAGG
CCTTACAGAAGACCTGGGTT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR216892 representing NM_027221
Red=Cloning site Green=Tags(s)

MRCCGVCAFDAARGPRRLMRVGLALILVGHVNLLVGAVLHGTVLRHVANPRGAVTPEYTTANVISVGSGL
LSVSVGLVALLASRNLLRPRLHWALLTLALVNLLLSAACSMGLLLAVSLTVANGGRRLIADCHPGLMDPS
IPLDQGPGHTDCSFDPTRIYDTALALWIPSLFMSAAEAALSGYCCVAALTLRGIGPCRKEGLQEQLQELT
ELELPECKRQENVQLLHGRQDFQALQKTWV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_027221
ORF Size 720 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_027221.3, NP_081497.2
RefSeq Size 922 bp
RefSeq ORF 723 bp
Locus ID 69815
UniProt ID Q8K177
Cytogenetics 5 B1
MW 26.1 kDa
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Documents
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"NM_027221" in other vectors (4)
SKU Description Size Price
MC211008 Krtcap3 (untagged) - Mouse keratinocyte associated protein 3 (Krtcap3), (10ug) 10 ug
$330.00
MG216892 Krtcap3 (tGFP-tagged) - Mouse keratinocyte associated protein 3 (Krtcap3), (10ug) 10 ug
$500.00
MR216892L3 Lenti ORF clone of Krtcap3 (Myc-DDK-tagged) - Mouse keratinocyte associated protein 3 (Krtcap3) 10 ug
$600.00
MR216892L4 Lenti ORF clone of Krtcap3 (mGFP-tagged) - Mouse keratinocyte associated protein 3 (Krtcap3) 10 ug
$600.00

Plasmid Map for MR216892

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR216892
Circular map for MR216892

file-alt Citations

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