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Nat3 (NM_008674) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR216865
Nat3 (Myc-DDK-tagged) - Mouse N-acetyltransferase 3 (Nat3)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Nat3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR216865 representing NM_008674
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACATTGAAGCATACTTTGAAAGAATTGGTTACCAGAAGTCCAGCAACAAACTGGACTTGCAGACAT
TAACTGAAATCCTTCAGCATCAGATACGGGCTATTCCTTTTGAGAACTTGAACATCCATTGTGGGAAAAC
CATGGAACTGAGCTTAGAGGACACCTTTCATCAAATTGTGAGGAAGAAGCGGGGAGGGTGGTGTCTTCAA
GTCAACCATCTTCTCTACTGGGCTCTGGCCATGATAGGGTTTGAGACCACAATGTTAGGAGGATGTGTTT
ATGTCCCTTCAGCCTGCAAGTATAGTAACACTATGATACACCTTCTACTACAGGTGACCATCAGTGGCAA
AACATATATTGTAGATAGTGCATTCCCATTTTCCTGCCAGCTATGGGAGCCTCTGGAGTTGACATCTGGG
AAGGATCAGCCTCAGGTTCCTGCCATCTTCCACCTGAGAGAAGAGAATGGAACCTGGTACCTGGAACAAA
CTAAAAGACAAGAATATGTTTCAAACCAAGAATTCATTGATTCTAATTTTCTTGAGAAGAACACACATCG
AAAAATATATTCTTTTACTCTTGAACCACGAACGATTGAAGATTTCTGGAGTATAAGTACATACTACCAG
GTATCTCGAACATCTGTGATGACAAACACATCACTTTGTTCCCTACATACCAAAGACGGAGTCCATGGCT
TAATGGGCACCATTCTTGCCTATAAGAAGTTCAATTATAAGGACAATATAGATCTGGTAGAGTTTAAGAC
TCTGAAGGAAGAAGAAATAGAAGAAGTCCTGAAGAGTGTTTTTGGAATTCACTTGGAGACAAAGCTTGTG
CCCAAATGTGGCAATGTCTTTTTTACTATT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR216865 representing NM_008674
Red=Cloning site Green=Tags(s)

MDIEAYFERIGYQKSSNKLDLQTLTEILQHQIRAIPFENLNIHCGKTMELSLEDTFHQIVRKKRGGWCLQ
VNHLLYWALAMIGFETTMLGGCVYVPSACKYSNTMIHLLLQVTISGKTYIVDSAFPFSCQLWEPLELTSG
KDQPQVPAIFHLREENGTWYLEQTKRQEYVSNQEFIDSNFLEKNTHRKIYSFTLEPRTIEDFWSISTYYQ
VSRTSVMTNTSLCSLHTKDGVHGLMGTILAYKKFNYKDNIDLVEFKTLKEEEIEEVLKSVFGIHLETKLV
PKCGNVFFTI

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008674
ORF Size 870 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008674.2, NP_032700.1
RefSeq Size 1395 bp
RefSeq ORF 873 bp
Locus ID 17962
UniProt ID P50296
Cytogenetics 8 33.39 cM
MW 33.7 kDa
Summary Participates in the detoxification of a plethora of hydrazine and arylamine drugs.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_008674" in other vectors (4)
SKU Description Size Price
MC209012 Nat3 (untagged) - Mouse N-acetyltransferase 3 (Nat3), (10ug) 10 ug
$330.00
MG216865 Nat3 (tGFP-tagged) - Mouse N-acetyltransferase 3 (Nat3), (10ug) 10 ug
$500.00
MR216865L3 Lenti ORF clone of Nat3 (Myc-DDK-tagged) - Mouse N-acetyltransferase 3 (Nat3) 10 ug
$600.00
MR216865L4 Lenti ORF clone of Nat3 (mGFP-tagged) - Mouse N-acetyltransferase 3 (Nat3) 10 ug
$600.00

Plasmid Map for MR216865

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR216865
Circular map for MR216865

file-alt Citations

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