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Adra2b (NM_009633) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR216706
Adra2b (Myc-DDK-tagged) - Mouse adrenergic receptor, alpha 2b (Adra2b)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Adra2b
Synonyms a2b-AR; Adra-2b; alpha2-C2; alpha2B; a2B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR216706 representing NM_009633
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCGGCCCCGCCATGGTCCACCAGGAGCCCTACTCGGTGCAGGCCACCGCCGCCATCGCGTCGGCCA
TCACCTTTCTCATCCTCTTCACCATTTTCGGCAATGCGCTGGTAATTCTGGCTGTGTTGACCAGCCGCTC
TCTCCGTGCACCACAAAACCTCTTCCTGGTGTCACTGGCAGCAGCCGACATCCTAGTGGCTACTCTTATC
ATCCCTTTCTCTCTGGCCAACGAGCTGCTGGGCTATTGGTACTTCTGGCGTGCGTGGTGCGAGGTCTACC
TGGCGCTAGACGTGCTCTTCTGTACCTCCTCCATCGTGCACCTGTGTGCCATCAGTCTGGACAGATACTG
GGCAGTGAGCCGAGCATTGGAGTACAACTCCAAGCGCACTCCACGCCGCATCAAATGCATCATCCTCACC
GTGTGGCTCATTGCAGCCGTCATTTCTCTACCGCCCCTCATCTACAAGGGCGACCAGCGCCCAGAGCCCC
ACGGGCTCCCCCAGTGTGAGCTCAACCAAGAAGCCTGGTACATCTTGGCTTCCAGCATCGGATCTTTTTT
TGCTCCCTGCCTCATCATGATTCTCGTCTACCTGCGAATCTATGTAATTGCCAAACGCAGCCACTGCAGA
GGTCTCGGAGCTAAGAGGGGCTCTGGGGAGGGTGAGTCCAAGAAGCCCCATCCGGCCGCCGGGGGAGTTC
CAGCCTCGGCTAAAGTGCCCACCCTGGTCTCTCCTCTATCTTCTGTCGGAGAGGCTAATGGACACCCCAA
GCCTCCAAGGGAGAAGGAGGAGGGGGAGACCCCTGAAGATCCCGAAGCCAGGGCTTTGCCACCCAATTGG
TCTGCCCTGCCCAGATCAGTCCAGGACCAGAAGAAGGGGACTAGTGGGGCAACTGCAGAGAAGGGAGCTG
AAGAGGATGAAGAGGAGGTGGAAGAATGTGAACCCCAAACACTGCCAGCTTCTCCTGCCTCAGTATTCAA
CCCACCCTTGCAGCAGCCTCAGACTTCCCGGGTGCTGGCCACGCTACGTGGCCAGGTGCTTCTGAGCAAG
AATGTGGGTGTGGCCAGTGGGCAGTGGTGGCGCCGTCGGACACAGCTGAGCCGAGAGAAGAGGTTCACCT
TTGTGCTGGCGGTGGTCATTGGTGTTTTTGTGGTCTGTTGGTTTCCTTTCTTCTTCAGCTACAGCCTGGG
GGCCATCTGCCCACAGCACTGCAAGGTACCCCATGGCCTCTTCCAGTTCTTCTTCTGGATTGGCTACTGC
AACAGCTCTTTGAATCCTGTCATCTACACCATCTTCAACCAGGACTTCCGCCGTGCCTTTCGAAGGATCC
TTTGCCGGCAGTGGACCCAGACTGGCTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR216706 representing NM_009633
Red=Cloning site Green=Tags(s)

MSGPAMVHQEPYSVQATAAIASAITFLILFTIFGNALVILAVLTSRSLRAPQNLFLVSLAAADILVATLI
IPFSLANELLGYWYFWRAWCEVYLALDVLFCTSSIVHLCAISLDRYWAVSRALEYNSKRTPRRIKCIILT
VWLIAAVISLPPLIYKGDQRPEPHGLPQCELNQEAWYILASSIGSFFAPCLIMILVYLRIYVIAKRSHCR
GLGAKRGSGEGESKKPHPAAGGVPASAKVPTLVSPLSSVGEANGHPKPPREKEEGETPEDPEARALPPNW
SALPRSVQDQKKGTSGATAEKGAEEDEEEVEECEPQTLPASPASVFNPPLQQPQTSRVLATLRGQVLLSK
NVGVASGQWWRRRTQLSREKRFTFVLAVVIGVFVVCWFPFFFSYSLGAICPQHCKVPHGLFQFFFWIGYC
NSSLNPVIYTIFNQDFRRAFRRILCRQWTQTGW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_009633
ORF Size 1359 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_009633.3, NP_033763.2
RefSeq Size 3936 bp
RefSeq ORF 1362 bp
Locus ID 11552
UniProt ID F8VQ23
Cytogenetics 2 61.95 cM
MW 50.9 kDa
Summary Alpha-2 adrenergic receptors mediate the catecholamine-induced inhibition of adenylate cyclase through the action of G proteins.UniProtKB/Swiss-Prot Function
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"NM_009633" in other vectors (4)
SKU Description Size Price
MC215916 Adra2b (untagged) - Mouse adrenergic receptor, alpha 2b (Adra2b), (10ug) 10 ug
$457.00
MG216706 Adra2b (tGFP-tagged) - Mouse adrenergic receptor alpha 2b (Adra2b), (10ug) 10 ug
$657.00
MR216706L3 Lenti ORF clone of Adra2b (Myc-DDK-tagged) - Mouse adrenergic receptor, alpha 2b (Adra2b) 10 ug
$757.00
MR216706L4 Lenti ORF clone of Adra2b (mGFP-tagged) - Mouse adrenergic receptor, alpha 2b (Adra2b) 10 ug
$757.00

Plasmid Map for MR216706

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR216706
Circular map for MR216706

file-alt Citations

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