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Clcnkb (NM_019701) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR216609
Clcnkb (Myc-DDK-tagged) - Mouse chloride channel Kb (Clcnkb)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$589.00 $629.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "Clcnkb or NM_019701"

Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Clcnkb
Synonyms Clc-Ka; Clck2; CLCKB; Clcnk1l
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR216609 representing NM_019701
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGAATTAGTGGGGCTTCGTGAGGGCGCCTCCAAGAAGCCAGTGCCACTGCAAGAACTCTGGGGGC
CATGCCCACGCATCCGCAGAAACATCCAGGGGGGCCTAGAGTGGCTGAAAGAGCGGCTCTTCCGTGTGGG
TGAGGACTGGTACTTCCTGGTGGCTCTCGGGGTGCTCATGGCTCTGATCAGCTATGCTATGAACTTCACT
ATTGGGCGCGTGGTCAGAGCACACAAGTGGCTGTACAGGGAGATTGGAGATGGCCACCTGCTCCGGTACC
TCTCCTGGACCGTGTACCCCGTGGCTCTTCTGTCCTTCTCCTCCGGCTTCTCACAGAGCATCACACCATC
CTCTGGAGGGTCTGGAATCCCTGAGGTGAAGACCATCTTGACCGGAGTGGTCCTGGAGGACTACCTAGAC
ATCAAGAACTTCGGGGCCAAGGTGGTGGGCCTCTCCTGCACCCTGGCCACAGGCAGTACCATCTTCCTGG
GAAAATTGGGTCCCTTCGTGCACCTGAGCGTGATGATCGCTGCTTACCTGGGCCGCGTGCGCACCAAGAC
CGTCGGGGAACCTGAGAGCAAGACCAAAGAAATGGAACTGTTGGCCGCAGGAGCAGCAGTGGGTGTGGCC
ACGGTCTTCGCAGCTCCCATCAGCGGTGTCCTGTTCAGCATCGAGGTCATGTCCTCTCACTTCTCCGTCT
GGGATTACTGGAGGGGCTTCTTCGCTGCCACCTGTGGGGCCTTTATGTTCCACCTCCTGGCAGTCTTCAA
CAATGAACAGGAGACCATCACCTCCATCTACAAGACCAGCTTTCCAGTGGACATACCCTTTGACTTACCA
GAGATCTTCTTTTTTGTGGCCCTGGGGGCCATCTGTGGCATCCTGAGCTGCGGGTACAACTACAGCCAGC
GGACTTTCTTATTTTTCCTCAAGGCCAATGGGTTCACCTCCAAACTGCTGGCTACCAGCAAACCCTTGTA
CTCAGCCCTGGCTGCTGTGGTCCTGGCTTCCATCACGTATCCCCCTGGTGTGGGCCACTTCATGGCTTCC
CGGCTGTCCATGTCGGAGCATTTGGAGACACTGTTTGACAACAACTCCTGGGCACTGATGACTAAGAACT
CGTCTCCACCCTGGGCAGCGGAGCCTGATCCCCAGAAGCTGTGGTTGGAGTGGTGTCACCCACAGTTGAC
TGTCTTTGGGACACTAGTCTTCTTCCTGGTCATGAAGTTCTGGATGCTGATTTTGGCAACCACCATCCCC
ATCCCTGCTGGATACTTCTTGCCCATCTTTATCTATGGAGCTGTCATTGGGCGCCTCTTTGGGGAGGTTC
TATCTGTCGCCTTCCCAGAGGGCATTGTGGCTGGAGGCAGGGTCAATCCCATCATGCCCGGGGCCTATGC
TCTGGCAGGTGCTGCTGCCTTCTCAGGGGCGGTGACCCACACCCTCTCCACGGCACTGCTGGCCTTTGAG
GTAACGGGCCAGCTCGTTCACGCACTGCCTGTGCTGATGGCTGTGCTGGCGGCCAACGCCATCTCTCAGA
GCTTCCAGCCCTCTTTCTACGATGGCACCATCATTGTCAAGAAACTGCCGTACCTGCCGTGGATCCGTGG
CCGACAGATTGGTTCCCACTCTGTAACTGTGGGTCACTTCATGAACTGCGCCCTCACTACACTGGCCAAG
GACATGCCCCTGGAGCAAGTGATCCAAGTCGTGATCTCCACAGATGTGACCCAGTACCCCCTGGTGGAGA
CTACAGAGTCTCAGACCCTGGTGGGCGTTGTAAAAAGGACCCACTTGGTGCAAGCCCTCCAGACTGAGCC
AGCTTCCTGGGCTCCAGGCCAGCAGCCCTGTCTCCAGGATATCTTGGCTAATGGCTGCCCCACCCAGCCA
GTGACACTGCAGCTGTCCCTGGAGACCTCCTTGCATGAGACACACAACCTTTTTGAGCTGCTGAACCTTC
AGACGCTGTTCGTGACGTCACGAGGCAGAGCTGTGGGCTCTGTATCGTGGGTGGAGCTGAAGAAAGCAAT
TTCAACCCTGACCAATCCTCCTGCCCCCAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR216609 representing NM_019701
Red=Cloning site Green=Tags(s)

MEELVGLREGASKKPVPLQELWGPCPRIRRNIQGGLEWLKERLFRVGEDWYFLVALGVLMALISYAMNFT
IGRVVRAHKWLYREIGDGHLLRYLSWTVYPVALLSFSSGFSQSITPSSGGSGIPEVKTILTGVVLEDYLD
IKNFGAKVVGLSCTLATGSTIFLGKLGPFVHLSVMIAAYLGRVRTKTVGEPESKTKEMELLAAGAAVGVA
TVFAAPISGVLFSIEVMSSHFSVWDYWRGFFAATCGAFMFHLLAVFNNEQETITSIYKTSFPVDIPFDLP
EIFFFVALGAICGILSCGYNYSQRTFLFFLKANGFTSKLLATSKPLYSALAAVVLASITYPPGVGHFMAS
RLSMSEHLETLFDNNSWALMTKNSSPPWAAEPDPQKLWLEWCHPQLTVFGTLVFFLVMKFWMLILATTIP
IPAGYFLPIFIYGAVIGRLFGEVLSVAFPEGIVAGGRVNPIMPGAYALAGAAAFSGAVTHTLSTALLAFE
VTGQLVHALPVLMAVLAANAISQSFQPSFYDGTIIVKKLPYLPWIRGRQIGSHSVTVGHFMNCALTTLAK
DMPLEQVIQVVISTDVTQYPLVETTESQTLVGVVKRTHLVQALQTEPASWAPGQQPCLQDILANGCPTQP
VTLQLSLETSLHETHNLFELLNLQTLFVTSRGRAVGSVSWVELKKAISTLTNPPAPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_019701
ORF Size 2061 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_019701.2, NP_062675.2
RefSeq Size 2344 bp
RefSeq ORF 2064 bp
Locus ID 56365
UniProt ID Q9WUB6
Cytogenetics 4 D3
MW 75.5 kDa
Summary This gene is a member of the CLC family of voltage-gated chloride channels. The gene is located adjacent to a highly similar chloride channel gene on chromosome 4. This gene is syntenic with human CLCNKA (geneID:1187). provided by RefSeq, Sep 2009
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"NM_019701" in other vectors (6)
SKU Description Size Price
MC220507 Clcnkb (untagged) - Mouse chloride channel Kb (Clcnkb), (10ug) 10 ug
$693.00
MG216609 Clcnkb (tGFP-tagged) - Mouse chloride channel Kb (Clcnkb), (10ug) 10 ug
$789.00 $829.00
MR216609L1 Lenti ORF clone of Clcnkb (Myc-DDK-tagged) - Mouse chloride channel Kb (Clcnkb) 10 ug
$929.00
MR216609L2 Lenti ORF clone of Clcnkb (mGFP-tagged) - Mouse chloride channel Kb (Clcnkb) 10 ug
$929.00
MR216609L3 Lenti ORF clone of Clcnkb (Myc-DDK-tagged) - Mouse chloride channel Kb (Clcnkb) 10 ug
$929.00
MR216609L4 Lenti ORF clone of Clcnkb (mGFP-tagged) - Mouse chloride channel Kb (Clcnkb) 10 ug
$929.00

Plasmid Map for MR216609

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR216609
Circular map for MR216609

file-alt Citations

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