Anxa3 (NM_013470) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR216256
Anxa3 (Myc-DDK-tagged) - Mouse annexin A3 (Anxa3)
$289.00 $300.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Anxa3
Synonyms Anx3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR216256 representing NM_013470
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTATCTGGGTTGGACCTCGAGGAACCATAAAAGATTATCCAGGCTTTAGCCCGTCGGTGGATG
CCGAAGCTATCCGGAAAGCGATCAGAGGACTTGGGACTGACGAGAAAACCCTCATCAACATTCTGACGGA
GCGGTCGAACGCGCAGCGACAGCTGATTGTCAAGCAGTACCAAGCAGCGTATGAACAGGAGCTGAAAGAT
GACTTGAAGGGTGATCTCTCTGGCCACTTCGAGCACGTCATGGTGGCTCTTGTTACTGCACCAGCCCTGT
TTGATGCGAAGCAACTGAAGAAATCCATGAAGGGAACTGGCACAGATGAAGATGCCCTGATTGAAATCCT
AACAACCAGGTCAAGCAGGCAGATGAAGGAAATCTCGCAGGCCTATTATACAGTGTATAAGAAGAGCCTC
GGGGATGACATTAGCTCTGAGACGTCTGGAGACTTCCGGAAAGCTCTGCTGACTCTGGCAGATGGTAGAA
GAGATGAAAGCCTCAAAGTGGATGAACATCTGGCCAAAAAGGATGCTCAGATCCTCTATAATGCTGGTGA
GAACAAATGGGGCACAGACGAAGACAAATTCACCGAGGTCTTGTGTCTACGGAGCTTCCCGCAGCTGAAA
CTAACATTTGATGAGTACAGAAATATTAGCCAGAAGGACATTGAGGACAGCATTAAAGGAGAATTATCTG
GACATTTTGAAGACCTGCTGCTGGCCATAGTTCATTGTGCGAGGAACACTCCAGCGTTTTTGGCAGAAAG
ACTTCATCAGGCTTTGAAGGGTGCTGGAACAGATGAATTCACTCTGAACAGAATAATGGTTTCCAGATCA
GAGATTGATCTTTTGGACATCAGACATGAGTTCAAGAAGCACTATGGCTACTCTCTATACTCGGCCATCC
AATCAGATACTTCTGGAGACTACAGAACCGTGCTCTTGAAGATCTGTGGAGAAGATGAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR216256 representing NM_013470
Red=Cloning site Green=Tags(s)

MASIWVGPRGTIKDYPGFSPSVDAEAIRKAIRGLGTDEKTLINILTERSNAQRQLIVKQYQAAYEQELKD
DLKGDLSGHFEHVMVALVTAPALFDAKQLKKSMKGTGTDEDALIEILTTRSSRQMKEISQAYYTVYKKSL
GDDISSETSGDFRKALLTLADGRRDESLKVDEHLAKKDAQILYNAGENKWGTDEDKFTEVLCLRSFPQLK
LTFDEYRNISQKDIEDSIKGELSGHFEDLLLAIVHCARNTPAFLAERLHQALKGAGTDEFTLNRIMVSRS
EIDLLDIRHEFKKHYGYSLYSAIQSDTSGDYRTVLLKICGEDD

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013470
ORF Size 969 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013470.2, NP_038498.2
RefSeq Size 1619 bp
RefSeq ORF 972 bp
Locus ID 11745
UniProt ID O35639
Cytogenetics 5 47.29 cM
MW 36.4 kDa
Summary Inhibitor of phospholipase A2, also possesses anti-coagulant properties.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_013470" in other vectors (4)
SKU Description Size Price
MC208131 Anxa3 (untagged) - Mouse annexin A3 (Anxa3), (10ug) 10 ug
$330.00
MG216256 Anxa3 (tGFP-tagged) - Mouse annexin A3 (Anxa3), (10ug) 10 ug
$500.00
MR216256L3 Lenti ORF clone of Anxa3 (Myc-DDK-tagged) - Mouse annexin A3 (Anxa3) 10 ug
$600.00
MR216256L4 Lenti ORF clone of Anxa3 (mGFP-tagged) - Mouse annexin A3 (Anxa3) 10 ug
$600.00

Plasmid Map for MR216256

Download sequence for

Cloning scheme for MR216256

Circular map for MR216256

file-alt Citations

Annexin A3 accelerates osteoclast differentiation by promoting the level of RANK and TRAF6
Lin, Li, Zhou et al
Bone (2023) 172, 116758 DOI: 10.1016/j.bone.2023.116758
Cited by 11
AI Summary: In this study, the pCMV6-ANXA3-Flag-Myc (MR216256) plasmid from OriGene was used to investigate the role of Annexin A3 in osteoclast differentiation and bone metabolism.
Snippet: pCMV6-ANXA3-Flag-Myc (MR216256) plasmid was purchased from OriGene (Rockville, MD, USA). pCMV3-TRAF6 (MG57197-UT) and pCMV3-RANK-HA (MG50490-CY) plasmids were purchased from Sino Biological (Beijing, China). The pCMV6-ANXA3-Myc, pCMV3
Showing 1-1 of 1 papers.
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