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Rhebl1 (NM_026967) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR215936
Rhebl1 (Myc-DDK-tagged) - Mouse Ras homolog enriched in brain like 1 (Rhebl1)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Rhebl1
Synonyms 1810036J22Rik; Rheb2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215936 representing NM_026967
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGCTGGTGCGCTACAGGAAGGTGGCCATCCTAGGGTACCGCTCCGTAGGGAAGACATCTTTGGCAC
ATCAATTTGTGGAAGGCGAGTTCCTGGAAGGCTACGATCCTACAGTGGAGAATACTTACAGCAAGACAGT
GACTCTTGGCAAAGATGAGTTTCACCTACATCTGGTGGACACAGCAGGGCAGGATGAGTACAGCATTCTG
CCCTACTCGCTCATCATCGGGGTCCATGGTTACGTTCTTGTATACTCGGTTAACTCTCTGCGCAGCTTTC
AGATCGTTAAGAACCTGTACCAAAAGCTACACGAAGGCCACGGTAAAACCCGGCTGTCGGTGCTGCTTGT
GGGGAACAAGGCAGATCTCTCTCCTGAGAGAGAGGTGCAGGCAGTTGAAGGGAAGAAGCTAGCAGAGTCC
TGGGGTGCGATGTTTATGGAGTCGTCTGCTCGGGATAATCAGCTAACTCAAGATGTCTTCATCAAAGTCA
TCCAGGAGATTGCCCGGGTGGAGAATTCTTATGGACGACAAGACCGCCGATGCTATCTTATG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR215936 representing NM_026967
Red=Cloning site Green=Tags(s)

MPLVRYRKVAILGYRSVGKTSLAHQFVEGEFLEGYDPTVENTYSKTVTLGKDEFHLHLVDTAGQDEYSIL
PYSLIIGVHGYVLVYSVNSLRSFQIVKNLYQKLHEGHGKTRLSVLLVGNKADLSPEREVQAVEGKKLAES
WGAMFMESSARDNQLTQDVFIKVIQEIARVENSYGRQDRRCYLM

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_026967
ORF Size 552 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_026967.4, NP_081243.1
RefSeq Size 1227 bp
RefSeq ORF 555 bp
Locus ID 69159
UniProt ID Q9D8T3
Cytogenetics 15 F1
MW 20.9 kDa
Summary Binds GTP and exhibits intrinsic GTPase activity. May activate NF-kappa-B-mediated gene transcription. Promotes signal transduction through MTOR, activates RPS6KB1, and is a downstream target of the small GTPase-activating proteins TSC1 and TSC2 (By similarity).UniProtKB/Swiss-Prot Function
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"NM_026967" in other vectors (4)
SKU Description Size Price
MC210860 Rhebl1 (untagged) - Mouse Ras homolog enriched in brain like 1 (Rhebl1), (10ug) 10 ug
$330.00
MG215936 Rhebl1 (tGFP-tagged) - Mouse Ras homolog enriched in brain like 1 (Rhebl1), (10ug) 10 ug
$500.00
MR215936L3 Lenti ORF clone of Rhebl1 (Myc-DDK-tagged) - Mouse Ras homolog enriched in brain like 1 (Rhebl1) 10 ug
$600.00
MR215936L4 Lenti ORF clone of Rhebl1 (mGFP-tagged) - Mouse Ras homolog enriched in brain like 1 (Rhebl1) 10 ug
$600.00

Plasmid Map for MR215936

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR215936
Circular map for MR215936

file-alt Citations

TAZ links exercise to mitochondrial biogenesis via mitochondrial transcription factor A
Hwang, Kim, Oh et al
Nat Commun (2022) 13 (1), 653 DOI: 10.1038/s41467-022-28247-2
Cited by 42
AI Summary: OriGene’s pCMV6-Rhebl1 plasmid (MR215936) was used as a template to amplify Rhebl1 coding sequences, enabling the study of Rhebl1’s role in TAZ-mediated mitochondrial biogenesis in skeletal muscle.
Snippet: ector was linearized by SnaBI digestion and mouse Rhebl1 coding sequences were amplified by Phusion polymerase (F530L, Thermo Fisher Scientific, Waltham, MA, USA) and pCMV6-Rhebl1 template (MR215936, Origene, Rockville, MD, USA). The amplified products were assembled with the linearized vector using Gibson assembly (NEBuilder High-Fidelity DNA Assembly Master Mix, E2621L, New England Biolabs, Ipsw
FigureFigureFigure
Showing 1-1 of 1 papers.
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