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Pdha2 (NM_008811) Mouse Tagged ORF Clone

  • TrueORF®
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SKU
MR215676
Pdha2 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase E1 alpha 2 (Pdha2)
Interest in protein/lysate? Submit request here!
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Pdha2
Synonyms Pdhal
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215676 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGAAAATGCTGACCGCTGTGCTGTCTCACGTATTTTCGGGAATGGTCCAAAAGCCAGCTCTCAGAG
GACTGCTGTCATCTCTGAAGTTCTCCAACGACGCCACCTGTGACATTAAGAAATGTGACCTGTACCGGCT
GGAGGAGGGCCCACCGACCTCCACCGTGCTCACCCGAGCCGAGGCCCTCAAGTACTACCGGACCATGCAG
GTAATTCGGCGCATGGAGTTGAAGGCCGACCAGCTGTATAAGCAGAAATTCATCCGTGGTTTCTGTCACC
TGTGTGATGGGCAGGAAGCCTGCTGCGTGGGGCTGGAGGCAGGGATAAATCCCACGGATCACGTCATCAC
GTCCTACCGGGCTCATGGCTTCTGCTACACGCGAGGACTGTCCGTGAAGTCCATTCTCGCCGAGCTGACT
GGACGCAAAGGAGGCTGTGCTAAAGGCAAGGGAGGCTCCATGCACATGTACGGCAAGAACTTCTACGGTG
GCAATGGCATTGTTGGGGCCCAGGTACCCCTGGGAGCTGGTGTGGCTTTTGCCTGTAAATACCTGAAGAA
TGGTCAGGTCTGCTTGGCTTTGTACGGCGATGGTGCGGCTAACCAAGGGCAGGTATTCGAAGCATACAAT
ATGTCAGCCTTGTGGAAATTACCCTGTGTTTTCATCTGTGAGAATAACCTCTATGGAATGGGAACCTCCA
ACGAGAGATCAGCAGCCAGTACTGATTACCACAAGAAAGGTTTTATTATCCCCGGACTGAGGGTGAATGG
GATGGATATTCTCTGTGTTCGGGAGGCAACCAAGTTTGCAGCTGATCACTGCAGATCTGGAAAGGGGCCC
ATTGTGATGGAGCTGCAGACCTACCGTTATCATGGACACAGTATGAGCGACCCAGGGATCAGTTATCGTT
CACGAGAAGAAGTTCATAACGTGAGAAGTAAGAGTGATCCTATAATGCTGCTCCGAGAGAGAATTATCAG
CAACAACCTCAGCAATATTGAAGAATTGAAAGAAATTGATGCAGATGTGAAGAAAGAGGTGGAGGACGCA
GCTCAGTTTGCTACGACTGATCCAGAACCAGCTGTGGAAGATATAGCCAATTACCTCTACCACCAAGATC
CACCTTTTGAAGTCCGTGGTGCACATAAGTGGCTCAAGTATAAGTCCCACAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>MR215676 protein sequence
Red=Cloning site Green=Tags(s)

MRKMLTAVLSHVFSGMVQKPALRGLLSSLKFSNDATCDIKKCDLYRLEEGPPTSTVLTRAEALKYYRTMQ
VIRRMELKADQLYKQKFIRGFCHLCDGQEACCVGLEAGINPTDHVITSYRAHGFCYTRGLSVKSILAELT
GRKGGCAKGKGGSMHMYGKNFYGGNGIVGAQVPLGAGVAFACKYLKNGQVCLALYGDGAANQGQVFEAYN
MSALWKLPCVFICENNLYGMGTSNERSAASTDYHKKGFIIPGLRVNGMDILCVREATKFAADHCRSGKGP
IVMELQTYRYHGHSMSDPGISYRSREEVHNVRSKSDPIMLLRERIISNNLSNIEELKEIDADVKKEVEDA
AQFATTDPEPAVEDIANYLYHQDPPFEVRGAHKWLKYKSHS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_008811
ORF Size 1173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_008811.2, NP_032837.1
RefSeq Size 2352 bp
RefSeq ORF 1176 bp
Locus ID 18598
UniProt ID P35487
Cytogenetics 3 H1
MW 43.4 kDa
Summary The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.UniProtKB/Swiss-Prot Function
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Documents
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"NM_008811" in other vectors (4)
SKU Description Size Price
MC209159 Pdha2 (untagged) - Mouse pyruvate dehydrogenase E1 alpha 2 (Pdha2), (10ug) 10 ug
$503.00
MG215676 Pdha2 (tGFP-tagged) - Mouse pyruvate dehydrogenase E1 alpha 2 (Pdha2), (10ug) 10 ug
$489.00 $657.00
MR215676L3 Lenti ORF clone of Pdha2 (Myc-DDK-tagged) - Mouse pyruvate dehydrogenase E1 alpha 2 (Pdha2) 10 ug
$757.00
MR215676L4 Lenti ORF clone of Pdha2 (mGFP-tagged) - Mouse pyruvate dehydrogenase E1 alpha 2 (Pdha2) 10 ug
$757.00

Plasmid Map for MR215676

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for MR215676
Circular map for MR215676

file-alt Citations

Specific autoantigens identified by sera obtained from mice that are immunized with testicular germ cells alone
Terayama, Hirai, Naito et al
Sci Rep (2016) 6, 35599 DOI: 10.1038/srep35599
AI Summary: OriGene contributed to this study by providing pCMV6 plasmid vectors encoding TGC-specific autoantigens, which were used to express recombinant proteins in HEK293 cells for antigenicity validation.
Snippet: Table 4 Table 4. Real time RT-PCR primers. Examination of expression ofTGC-specific AIs gene by Human embryonic kidney 293 (HEK293) cells. Plasmid vectors (pCMV6)of TGC-specific AIs and Native were purchased from Origene (Table S2). ... Cite SaveSaving
FigureFigureFigure
Showing 1-1 of 1 papers.
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