Cln5 (NM_001033242) Mouse Tagged ORF Clone

  • TrueORF®
SKU
MR215618
Cln5 (Myc-DDK-tagged) - Mouse ceroid-lipofuscinosis, neuronal 5 (Cln5)
  $686.00
In Stock*
Specifications
Specifications
Product Data
Type Mouse Tagged ORF Clone
Target Symbol Cln5
Synonyms A730075N08Rik
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>MR215618 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGCGGGGGGCCATGTGGAGCGCACTGGCGCCCGGCGCTCGCGCTGGCGCTGCTCGGGCTGGCGA
CGATTCTGGGCGCGTCCCCGACGTCTGGGCAACGCTGGCCGGTGCCCTACAAGCGCTTCTCTTTCCGTCC
GAAGACAGATCCCTACTGTCAAGCTAAGTATACTTTCTGTCCTACCGGCTCGCCCATCCCAGTTATGAAG
GACAATGACGTCATCGAGGTCTTACGACTACAAGCCCCGATTTGGGAATTTAAATATGGAGACCTCCTGG
GACACTTTAAACTTATGCATGACGCCGTGGGATTCAGGAGCACACTGACAGGCAAGAACTACACAATTGA
GTGGTATGAACTTTTCCAGCTGGGCAACTGTACATTTCCCCACCTCCGGCCTGACAAGAGCGCTCCCTTC
TGGTGTAACCAAGGGGCAGCCTGCTTTTTTGAAGGAATAGATGATAAACACTGGAAGGAAAACGGGACAC
TGTCAGTCGTTGCAACCATATCCGGAAACACATTTAACAAAGTGGCCGAGTGGGTGAAGCAGGACAATGA
AACTGGGATTTATTATGAGACATGGACGGTCCGAGCCGGCCCAGGACAAGGGGCCCAGACGTGGTTCGAG
TCCTACGACTGTTCGAATTTTGTCTTAAGGACATATAAGAAATTGGCTGAATTTGGAACAGAATTCAAGA
AGATAGAAACAAACTATACGAAAATATTTCTTTACAGTGGAGAGCCTATTTACCTGGGAAATGAAACATC
TATTTTTGGGCCCAAAGGAAACAAGACTCTTGCTTTGGCCATAAAAAAATTTTATGGCCCCTTCAGACCG
TATTTGTCAACCAAAGATTTTCTGATGAATTTCTTGAAAATTTTTGATACAGTGATTATACACAGACAGT
TCTACTTGTTTTATAACTTTGAGTATTGGTTTCTACCAATGAAACCCCCCTTTGTCAAAATAACATACGA
AGAAACCCCGTTACCTACCCGACATACAACATTTACCGACTTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>MR215618 protein sequence
Red=Cloning site Green=Tags(s)

MLRGGPCGAHWRPALALALLGLATILGASPTSGQRWPVPYKRFSFRPKTDPYCQAKYTFCPTGSPIPVMK
DNDVIEVLRLQAPIWEFKYGDLLGHFKLMHDAVGFRSTLTGKNYTIEWYELFQLGNCTFPHLRPDKSAPF
WCNQGAACFFEGIDDKHWKENGTLSVVATISGNTFNKVAEWVKQDNETGIYYETWTVRAGPGQGAQTWFE
SYDCSNFVLRTYKKLAEFGTEFKKIETNYTKIFLYSGEPIYLGNETSIFGPKGNKTLALAIKKFYGPFRP
YLSTKDFLMNFLKIFDTVIIHRQFYLFYNFEYWFLPMKPPFVKITYEETPLPTRHTTFTDL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene |
ACCN NM_001033242
ORF Size 1023 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001033242.1, NP_001028414.1
RefSeq Size 2447 bp
RefSeq ORF 1026 bp
Locus ID 211286
UniProt ID Q3UMW8
Cytogenetics 14 51.71 cM
MW 39.3 kDa
Summary Plays a role in influencing the retrograde trafficking of lysosomal sorting receptors SORT1 and IGF2R from the endosomes to the trans-Golgi network by controlling the recruitment of retromer complex to the endosomal membrane. Regulates the localization and activation of RAB7A which is required to recruit the retromer complex to the endosomal membrane.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001033242" in other vectors (4)
SKU Description Size Price
MC212551 Cln5 (untagged) - Mouse ceroid-lipofuscinosis, neuronal 5 (Cln5), (10ug) 10 ug
$686.00
MG215618 Cln5 (tGFP-tagged) - Mouse ceroid-lipofuscinosis neuronal 5 (Cln5), (10ug) 10 ug
$886.00
MR215618L3 Lenti ORF clone of Cln5 (Myc-DDK-tagged) - Mouse ceroid-lipofuscinosis, neuronal 5 (Cln5) 10 ug
$986.00
MR215618L4 Lenti ORF clone of Cln5 (mGFP-tagged) - Mouse ceroid-lipofuscinosis, neuronal 5 (Cln5) 10 ug
$986.00

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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